ID   3SX_NAJKA               Reviewed;          61 AA.
AC   P0DSN0;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Nakoroxin {ECO:0000303|PubMed:28864897};
DE   AltName: Full=Naja kaouthia orphan toxin {ECO:0000303|PubMed:28864897};
DE   AltName: Full=Three-finger toxin;
DE            Short=3FTx;
OS   Naja kaouthia (Monocled cobra) (Naja siamensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8649;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=28864897; DOI=10.1134/s1607672917040068;
RA   Osipov A.V., Meshcheryakova A.V., Starkov V.G., Ziganshin R.K.,
RA   Oustitch T.L., Peters L.E., Tsetlin V.I., Utkin Y.N.;
RT   "New paradoxical three-finger toxin from the cobra Naja kaouthia venom:
RT   isolation and characterization.";
RL   Dokl. Biochem. Biophys. 475:264-266(2017).
CC   -!- FUNCTION: Shows no cytotoxicity and does not inhibit the binding of
CC       alpha-bungarotoxin to nicotinic acetylcholine receptors of muscle and
CC       alpha-7/CHRNA7 types. However, it potentiates the binding of alpha-
CC       bungarotoxin to the acetylcholine-binding protein from Lymnaea
CC       stagnalis. {ECO:0000269|PubMed:28864897}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28864897}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:28864897}.
CC   -!- MASS SPECTROMETRY: Mass=6845.35; Method=Unknown;
CC       Evidence={ECO:0000269|PubMed:28864897};
CC   -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC       residue stands at position 28 (Pro-31 in standard classification).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DSN0; -.
DR   SMR; P0DSN0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Toxin.
FT   CHAIN           1..61
FT                   /note="Nakoroxin"
FT                   /evidence="ECO:0000269|PubMed:28864897"
FT                   /id="PRO_0000447298"
FT   DISULFID        3..19
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        12..37
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        41..49
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        50..55
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
SQ   SEQUENCE   61 AA;  6854 MW;  8989013DA7B1ECE9 CRC64;
     MECYRMSNIV TCQPWEKFCY KEVTMFFPNH PVHLSGCASE CTETNSKFCC TTDKCNGAGS
     G