ID   HTR1_MAGO7              Reviewed;         198 AA.
AC   Q2KEJ2; A4QX86;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Host transcription reprogramming factor 1 {ECO:0000303|PubMed:33203871};
DE   AltName: Full=Secreted nuclear effector HTR1 {ECO:0000303|PubMed:33203871};
DE   Flags: Precursor;
GN   Name=HTR1 {ECO:0000303|PubMed:33203871};
GN   ORFNames=MGCH7_ch7g1044, MGG_10276;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [2]
RP   FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=33203871; DOI=10.1038/s41467-020-19624-w;
RA   Kim S., Kim C.Y., Park S.Y., Kim K.T., Jeon J., Chung H., Choi G., Kwon S.,
RA   Choi J., Jeon J., Jeon J.S., Khang C.H., Kang S., Lee Y.H.;
RT   "Two nuclear effectors of the rice blast fungus modulate host immunity via
RT   transcriptional reprogramming.";
RL   Nat. Commun. 11:5845-5845(2020).
CC   -!- FUNCTION: Secreted effector that translocates into the nuclei of host
CC       cells to reprogram the expression of immunity-associated genes by
CC       binding on effector binding elements (EBEs) in rice (PubMed:33203871).
CC       Binds the 5'-CAATCTTC-3' EBE of promoters from targeted rice genes and
CC       probably recruits a yet to determine host repressor (PubMed:33203871).
CC       Causes ambivalent immunity with increased susceptibility to the
CC       hemibiotrophic pathogens Magnaporthe oryzae and Xanthomonas oryzae pv.
CC       oryzae, but enhances resistance to Cochliobolus miyabeanus, a
CC       necrotrophic pathogen (PubMed:33203871). {ECO:0000269|PubMed:33203871}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33203871}. Host
CC       nucleus {ECO:0000269|PubMed:33203871}. Note=Secreted via the biotrophic
CC       interfacial complex (BIC) and translocated into the nuclei of initially
CC       penetrated and surrounding cells. {ECO:0000269|PubMed:33203871}.
CC   -!- INDUCTION: Expressed during multiple stages of host plant infection,
CC       including the prepenetration, early biotrophy, late biotrophy,
CC       transition and necrotrophy. {ECO:0000269|PubMed:33203871}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect vegetative growth, conidiation,
CC       conidial germination, nor appressorium formation, but leads to
CC       significantly reduced virulence to rice. {ECO:0000269|PubMed:33203871}.
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DR   EMBL; CM000230; EAQ71637.1; -; Genomic_DNA.
DR   EMBL; JH165178; KYQ30505.1; -; Genomic_DNA.
DR   RefSeq; XP_016846009.1; XM_016990522.1.
DR   AlphaFoldDB; Q2KEJ2; -.
DR   EnsemblFungi; MGG_10276T0; MGG_10276T0; MGG_10276.
DR   GeneID; 2681827; -.
DR   KEGG; mgr:MGG_10276; -.
DR   VEuPathDB; FungiDB:MGG_10276; -.
DR   HOGENOM; CLU_1378381_0_0_1; -.
DR   Proteomes; UP000009058; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE   2: Evidence at transcript level;
KW   Host nucleus; Metal-binding; Reference proteome; Repressor; Secreted;
KW   Signal; Transcription; Transcription regulation; Virulence; Zinc;
KW   Zinc-finger.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..198
FT                   /note="Host transcription reprogramming factor 1"
FT                   /id="PRO_5011098161"
FT   ZN_FING         58..81
FT                   /note="C2H2-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          21..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          71..198
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        99..149
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   198 AA;  21716 MW;  9067A74C3FC603E7 CRC64;
     MQLSNFLSIW ALVAMGATAA PMPSGSAPGN PFADGEAYGA RPSQGLTPVP KVHDGSYHSC
     ETCAAPFRTE ERLAAHRQAD HGNQGAAARG SGRHTSKNRG SSKTSERERL DRLASRVGED
     YVEKRSQEAT EEQEQGHVKP GRSKKEIAQR IRNLPVTPWN QYGAAVKLDK PTRKEQYRQL
     PGNDRLTPFE EQGDPVQL