HTR4_HALS3
ID HTR4_HALS3 Reviewed; 778 AA.
AC B0R470; Q48319; Q9HR92;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Transducer protein Htr4;
GN Name=htr4; OrderedLocusNames=OE_2189R;
OS Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium.
OX NCBI_TaxID=478009;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=R1 / S9;
RX PubMed=8674984; DOI=10.1111/j.1574-6968.1996.tb08197.x;
RA Rudolph J., Nordmann B., Storch K.F., Gruenberg H., Rodewald K.,
RA Oesterhelt D.;
RT "A family of halobacterial transducer proteins.";
RL FEMS Microbiol. Lett. 139:161-168(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29341 / DSM 671 / R1;
RX PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT R1 compared to that of strain NRC-1.";
RL Genomics 91:335-346(2008).
RN [3]
RP METHYLATION AT GLU-522 AND GLU-739.
RC STRAIN=R1 / S9;
RX PubMed=18514223; DOI=10.1016/j.jmb.2008.04.063;
RA Koch M.K., Staudinger W.F., Siedler F., Oesterhelt D.;
RT "Physiological sites of deamidation and methyl esterification in sensory
RT transducers of Halobacterium salinarum.";
RL J. Mol. Biol. 380:285-302(2008).
CC -!- FUNCTION: Potentially involved in chemo- or phototactic signal
CC transduction. {ECO:0000269|PubMed:8674984}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- PTM: Methylated by CheR. {ECO:0000269|PubMed:18514223}.
CC -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC family. {ECO:0000305}.
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DR EMBL; X95590; CAA64843.1; -; Genomic_DNA.
DR EMBL; AM774415; CAP13535.1; -; Genomic_DNA.
DR PIR; T48897; T48897.
DR RefSeq; WP_010902562.1; NC_010364.1.
DR AlphaFoldDB; B0R470; -.
DR SMR; B0R470; -.
DR BindingDB; B0R470; -.
DR EnsemblBacteria; CAP13535; CAP13535; OE_2189R.
DR GeneID; 5952701; -.
DR KEGG; hsl:OE_2189R; -.
DR HOGENOM; CLU_000445_107_19_2; -.
DR OMA; MWNEQNE; -.
DR PhylomeDB; B0R470; -.
DR Proteomes; UP000001321; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004090; Chemotax_Me-accpt_rcpt.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR004089; MCPsignal_dom.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF00015; MCPsignal; 1.
DR PRINTS; PR00260; CHEMTRNSDUCR.
DR SMART; SM00304; HAMP; 2.
DR SMART; SM00283; MA; 1.
DR PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
DR PROSITE; PS50885; HAMP; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Chemotaxis; Membrane; Methylation; Repeat; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..778
FT /note="Transducer protein Htr4"
FT /id="PRO_0000429076"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..296
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 318..778
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 318..370
FT /note="HAMP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 412..465
FT /note="HAMP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 484..720
FT /note="Methyl-accepting transducer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
FT REGION 376..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 522
FT /note="Glutamate methyl ester (Glu)"
FT /evidence="ECO:0000269|PubMed:18514223"
FT MOD_RES 739
FT /note="Glutamate methyl ester (Glu)"
FT /evidence="ECO:0000269|PubMed:18514223"
FT CONFLICT 467..469
FT /note="VVA -> AGR (in Ref. 1; CAA64843)"
FT /evidence="ECO:0000305"
FT CONFLICT 742
FT /note="T -> S (in Ref. 1; CAA64843)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 778 AA; 82077 MW; D752278727A4FA79 CRC64;
MSIRSFAHRI LRRALPGFVR RSYLAKFGVA LLAVVVCISA AGGVMYLNTS EHLQQSSRTE
LKKAAELSSS AVDNWHDERT NNARMLAQYG VFDNDNATEV QQFFTDEQHH LPSDVRDIHY
VSLTDARVIT STDAGLRNAS FGSEAAPWSR QQLTPGDDGV FVSQPYVNDG ITEVAYVARV
SSTPGRRTAV VMTASLAAIS SSFWDPTPHS FTQLVDGTGS VIADDSKRAT RQPYVENATS
PIVGARAVGF QPAAQAAKEM DQAHETAYAP VDGTPWVVTL HVPTSEAYGL ASNMAENVLL
ILGIALAGLV FIALTLGRGT VRALNDLEAK AAALERGEYD TDLDVARVDE LGRLFEAFAS
LRDTVQARIR DANEQQVDAE AARSEAEAAQ ADAEAAQAEA EAAREESEAQ ARRLETTAEA
FSETMRAYAA GDLTVRLDAD VEQAAMADIA AAFNEMAADM EATIADVVAF ADEVATASTD
ASDSAAAVEQ TGRDVSDAVG RIRDRAADQR DQLEAVASET DEMSATIEEV AASADQVAET
SQRAAALGDD GQAAAQDAVA QLEEIEDETQ AAATAVDDLE AKMSEIETIV AAITDIAEQT
NMLALNANIE AARADQDGDG FAVVADEVKD LADESKASAA EIEALVAEVR AQTETSVAAM
DRIQERVSDG VETVSETERS LSEIAGRIAE ADTGVQEISN AMDDQAASVS DVTTAVGDVA
ALGEETATEA ESTADAAAEQ ATTLSDVAAQ TETLAEHAVA LREHAAQFEV AADNEPGA
