HTR5A_MOUSE
ID HTR5A_MOUSE Reviewed; 2038 AA.
AC Q5PRF0; E9QPI0; Q3TT84; Q3V311; Q6P4S9; Q80TF2; Q8C8F7;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=HEAT repeat-containing protein 5A;
GN Name=Heatr5a; Synonyms=Kiaa1316;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-1248 AND 1480-2038 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Embryonic head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 598-2038 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5PRF0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5PRF0-2; Sequence=VSP_029687;
CC -!- SIMILARITY: Belongs to the HEATR5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC32998.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAE43352.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK047221; BAC32998.1; ALT_FRAME; mRNA.
DR EMBL; AK053055; BAE43352.1; ALT_FRAME; mRNA.
DR EMBL; AK161523; BAE36441.1; -; mRNA.
DR EMBL; AC157213; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC161114; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC063265; AAH63265.1; -; mRNA.
DR EMBL; BC086656; AAH86656.1; -; mRNA.
DR EMBL; AK122493; BAC65775.1; -; mRNA.
DR CCDS; CCDS25902.1; -. [Q5PRF0-1]
DR RefSeq; NP_796145.2; NM_177171.4. [Q5PRF0-1]
DR AlphaFoldDB; Q5PRF0; -.
DR STRING; 10090.ENSMUSP00000043115; -.
DR iPTMnet; Q5PRF0; -.
DR PhosphoSitePlus; Q5PRF0; -.
DR EPD; Q5PRF0; -.
DR MaxQB; Q5PRF0; -.
DR PaxDb; Q5PRF0; -.
DR PeptideAtlas; Q5PRF0; -.
DR PRIDE; Q5PRF0; -.
DR ProteomicsDB; 273348; -. [Q5PRF0-1]
DR ProteomicsDB; 273349; -. [Q5PRF0-2]
DR Antibodypedia; 63890; 11 antibodies from 6 providers.
DR Ensembl; ENSMUST00000040583; ENSMUSP00000043115; ENSMUSG00000035181. [Q5PRF0-1]
DR GeneID; 320487; -.
DR KEGG; mmu:320487; -.
DR UCSC; uc007nna.1; mouse. [Q5PRF0-1]
DR UCSC; uc007nnc.1; mouse. [Q5PRF0-2]
DR CTD; 25938; -.
DR MGI; MGI:2444133; Heatr5a.
DR VEuPathDB; HostDB:ENSMUSG00000035181; -.
DR eggNOG; KOG1822; Eukaryota.
DR GeneTree; ENSGT00390000006205; -.
DR HOGENOM; CLU_000652_0_0_1; -.
DR InParanoid; Q5PRF0; -.
DR OMA; GIVCAHV; -.
DR OrthoDB; 71064at2759; -.
DR PhylomeDB; Q5PRF0; -.
DR TreeFam; TF300706; -.
DR BioGRID-ORCS; 320487; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Heatr5a; mouse.
DR PRO; PR:Q5PRF0; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q5PRF0; protein.
DR Bgee; ENSMUSG00000035181; Expressed in otolith organ and 220 other tissues.
DR ExpressionAtlas; Q5PRF0; baseline and differential.
DR Genevisible; Q5PRF0; MM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0030139; C:endocytic vesicle; IBA:GO_Central.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR040108; Laa1/Sip1/HEATR5.
DR PANTHER; PTHR21663; PTHR21663; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..2038
FT /note="HEAT repeat-containing protein 5A"
FT /id="PRO_0000311991"
FT REPEAT 850..887
FT /note="HEAT 1"
FT REPEAT 1082..1119
FT /note="HEAT 2"
FT REGION 1646..1668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1646..1664
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1647
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86XA9"
FT VAR_SEQ 654..2038
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029687"
FT CONFLICT 57
FT /note="L -> V (in Ref. 1; BAC32998)"
FT /evidence="ECO:0000305"
FT CONFLICT 686
FT /note="E -> G (in Ref. 1; BAE43352)"
FT /evidence="ECO:0000305"
FT CONFLICT 737
FT /note="D -> N (in Ref. 1; BAE43352)"
FT /evidence="ECO:0000305"
FT CONFLICT 1070
FT /note="S -> G (in Ref. 1; BAE43352)"
FT /evidence="ECO:0000305"
FT CONFLICT 1643
FT /note="E -> G (in Ref. 3; AAH86656)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2038 AA; 219893 MW; 826E013A2C8002D5 CRC64;
MELAHSLLLN EEASNQLGAV QKAEFIFEWL RYLEKLLLAT NREDVREKQK TLVGQLLSLL
NSSPGPPTRK LLAQDLAILY SVGDTVSVYE TIDKCNDLIR SKDDSPSYLP TKLAAVVCLG
SLYKKLGRIL ANGFTDTVVN ILKAMKSAES QGRYEIMLSL QSILTGLGAA AAPCHRDVYK
AARSCLTDRS MAVRCAAAKC LLELQNEAIF MWSTDVDSVA TLCFKSFEGS NYDVRISVSK
LLGTVLAKAV TAKHPGAGSK QSARRVSLEE VLELLGAGFL RGSSGFLRAS GDMLKGNSSV
SRDVRVGVTQ AYVVFVSTLG GAWLEKNLAA FLSHILSLVS QSNPKATQTQ IDAVCCRRCV
SFILRATLGG LLGEKAQIAA AKEICQAVWR LKKVMDAALS DGNVETRLSS TDVAASQHVL
VCALQELGNL IHNLGTTAAP LLQDSSTGLL DSVISVVLHP SISVRLAAAW CLHCIAVALP
SYLTPLLDRC LERLAILKSS PEAVTGFSSA VAALLGSVTH CPLGIPHGKG KIIMTIAEDL
LCSAAQNSRL SLQRTQAGWL LVAALMTLGP AVVSHHLTRV LLLWKCVFPA SPRDLETEKS
RGDSFTWQVT LEGRAGALCA VKSFISHCGD LLTEEVIQRL LPPLPCAVDL LTQLSSILKT
YGSSLKTPSI VYRQRLYELL ILLPPETYKG NLCVILKELA AELTAPDTQA AASTCLLPAL
CHPDDLLILS PLLQETDHRF IEEQLLLGNG VACGSLEYDP YSIYEKDVEG DSVPKPLPPA
LSVISSASKL FGVVCATVDE AQRVLILEQL LNSIKHTKGA RQQTVQLHVV SAISNLLKYV
AGSKQSLGPE VRRLVLTLVL GALESPTPLL RCAASEAWAR LAQVADDGAF TAGLAQLSFD
KLKSARDVVT RTGHSLALGS LHRYLGGIGP QHLSSCIGVL YTLSQDSTSP DVQTWALHSL
SLTIDSAGAL YHVHVESTLS LIVMLLLNVP PTHAQVHQSL GRCLNALITT LGPELQGSNT
SVSALRTSCL LGCAVMQDHP GCLVQAQAIS CLQQLHMFAP RHVNLSSLVS CLCVNLCSPY
LLLRRAVLAC LRQLVQREAA EVSEHAIMLA RDGRDAAADA NLREVGLEGA LLALLDRETD
ESLCQDIRET LHHMLTSMAV GKLTLWLKLC KDVLAASADF TAVTCVDTMQ EEEGDRGDDA
SVLTRGDDKP HPFSNPRWAT RVFAADCVCR IINQCENANR AHFDIALAQE MKKRDSRNDF
LVLHLADLIR MAFMAATDHS DQLRLSGLDT LLVVIRRFAD IAEPEFPGHV ILEQYQANVG
AALRPAFTSE TPPDITAKAC QVCSAWIASG VVSDLSDLRR VHQLLVSSLT KIQAGKEALS
QLYNESASTM EILAVLRAWA EVYIIAVQRH KNHKQALKTT VNSEDSMRNG SSSAAGLLDL
VCTDLATLSK LWLAALQDFA LLTLPAEFAS QLPTEGGAFY TAETSKSAKL HYHDSWALIL
HAAALWLTST GFADPDEGGA NLSRPVTPTS MCQGSSSSGA AVKSPEDVYT DRFHLILGIS
VEFLCSLRSD ASLESIMACL RALQALLDVP WPRWRIGSDQ DLGIELLNVL HRVILTRESP
AIQLASLEVV RQIICAAQEH VKEKRRSAEV DDGASEKETL PEFGEGKDTG GLVPGKSLVF
ATLELCVCIL VRQLPELNPK LAGSPGGKAS KPKTLLEEGS RLVAAALAIL AELPAVCSPE
GSISILPTVL YLTIGVLRET AVKLPGGQLS CTVTASLQTL KGILTSPMAR AEKSHEAWTS
LLQSALATVL DCWSPVDGAQ EPDEVSLLTA VTVFILSTSP EVTTVPCLQN RCIEKFKAAL
ESKDSVVQMK TCQLLHSIFQ YPKPAVSYPY IYSLASSIVE KLQDIARRKP EDATELQLCQ
EGIKLLEALV AIAEEEHRAQ LVACLLPILI SFLLDENALG SATSVTRSLH DFALHSLMQI
GPRYSSVFKR VMASSPALKA RLEAAVKGNQ ESVRVDPPSK HAKNLARNSS IQLKTNFL
