ID   HTRA4_BOVIN             Reviewed;         484 AA.
AC   E1BJW1;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Serine protease HTR4;
DE            EC=3.4.21.-;
DE   AltName: Full=High-temperature requirement factor A4;
DE   Flags: Precursor;
GN   Name=HTRA4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hereford;
RX   PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA   Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA   Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA   Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT   "A whole-genome assembly of the domestic cow, Bos taurus.";
RL   Genome Biol. 10:R42.01-R42.10(2009).
CC   -!- FUNCTION: Serine protease. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1C family. {ECO:0000305}.
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DR   EMBL; DAAA02060867; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; E1BJW1; -.
DR   SMR; E1BJW1; -.
DR   STRING; 9913.ENSBTAP00000004564; -.
DR   PaxDb; E1BJW1; -.
DR   PRIDE; E1BJW1; -.
DR   eggNOG; ENOG502RMZV; Eukaryota.
DR   HOGENOM; CLU_020120_6_2_1; -.
DR   InParanoid; E1BJW1; -.
DR   OrthoDB; 630723at2759; -.
DR   TreeFam; TF323480; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; IBA:GO_Central.
DR   GO; GO:0012501; P:programmed cell death; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR   InterPro; IPR000867; IGFBP-like.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR001940; Peptidase_S1C.
DR   Pfam; PF00219; IGFBP; 1.
DR   Pfam; PF07648; Kazal_2; 1.
DR   Pfam; PF17820; PDZ_6; 1.
DR   PRINTS; PR00834; PROTEASES2C.
DR   SMART; SM00121; IB; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   SUPFAM; SSF57184; SSF57184; 1.
DR   PROSITE; PS51323; IGFBP_N_2; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
DR   PROSITE; PS50106; PDZ; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hydrolase; Protease; Reference proteome; Secreted;
KW   Serine protease; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..484
FT                   /note="Serine protease HTR4"
FT                   /id="PRO_0000417599"
FT   DOMAIN          36..114
FT                   /note="IGFBP N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00653"
FT   DOMAIN          105..159
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DOMAIN          390..472
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   REGION          209..369
FT                   /note="Serine protease"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        225
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        255
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        333
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   DISULFID        113..132
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        121..157
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ   SEQUENCE   484 AA;  51220 MW;  070870AB42A50BF4 CRC64;
     MARPLQRPAG LGPFVLLWLL LPAPSGRGVQ AGRAWPVPRC PAACEPTRCP PLPRCSVGTA
     PVLDRCGCCR VCAAAEGEAC GGPLGRPCAP GLQCLAPRAP RLLGGRPLGT CGCPAAGATV
     CGSDGRTYRS LCALRAENRA ARLRGALPAV PVQKGDCGDP GTGSAGWLRN KFNFIASVVE
     KVAPSVVHLQ LFRRDRSPLG SEDVPVSSAS GFIVSEDGLI VTNAHVLTNQ QRIQVELQSG
     VQYEATVKDV DHKLDLALIK IEPNADLPVL LLGKSSDLRA GEFVVALGSP FSLQNTVTAG
     IVSTTQRGGK ELGLKDSDMD YIQTDAIINH GNSGGPLVNL DGDVIGINTL KVTAGISFAI
     PSDRIRQFLA EFHERQLKGK ALSQKKYLGL RMLPLTMNLL QEMKRQDPEF PDVASGVFVH
     EVIQGTAAES SGLKDHDVIV SINGLPVTTT TDVIEAVKAN DSLSLLVRRK SQTLILTVTP
     EIIN