ID   HTRA_LACHE              Reviewed;         413 AA.
AC   Q9Z4H7;
DT   11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2001, sequence version 2.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Serine protease Do-like HtrA;
DE            EC=3.4.21.107;
GN   Name=htrA;
OS   Lactobacillus helveticus (Lactobacillus suntoryeus).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=1587;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=53/7;
RX   PubMed=9829922; DOI=10.1128/jb.180.23.6148-6153.1998;
RA   Smeds A., Varmanen P.K., Palva A.M.;
RT   "Molecular characterization of a stress-inducible gene from Lactobacillus
RT   helveticus.";
RL   J. Bacteriol. 180:6148-6153(1998).
CC   -!- FUNCTION: Degrades abnormal exported proteins and responsible for the
CC       propeptide processing of a natural pro-protein and for the maturation
CC       of a native protein. It also plays a prominent role in stress (heat
CC       shock, ethanol, puromycin and NaCl) resistance during active
CC       exponential growth (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Acts on substrates that are at least partially unfolded. The
CC         cleavage site P1 residue is normally between a pair of hydrophobic
CC         residues, such as Val-|-Val.; EC=3.4.21.107;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S1C family. {ECO:0000305}.
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DR   EMBL; AJ005672; CAA06668.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9Z4H7; -.
DR   SMR; Q9Z4H7; -.
DR   STRING; 326425.lhe_0111; -.
DR   MEROPS; S01.447; -.
DR   eggNOG; COG0265; Bacteria.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001940; Peptidase_S1C.
DR   Pfam; PF13180; PDZ_2; 1.
DR   PRINTS; PR00834; PROTEASES2C.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50106; PDZ; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Protease; Serine protease;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..413
FT                   /note="Serine protease Do-like HtrA"
FT                   /id="PRO_0000093860"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          305..401
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   ACT_SITE        150
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        180
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        258
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   BINDING         256..258
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         316..320
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   413 AA;  42647 MW;  B16B677991C88707 CRC64;
     MVENQNNNQN QPRKKSGNKI IATAAIFGVV GGLVGGGVSY YAMDQMNNGQ GNGAAQISIS
     SSSSKVSEKS AKNGGTMTAA YNDVKGAVVS VINLKRQSAS SGTDSLYNSL FGDDSDSSSS
     KNGKLETYSE GSGVVYMKSN GKGYIVTNNH VISGSDAVQV LLANGKTVNA KVVGKDSTTD
     LAVLSIDAKY VTQTAQFGDS KHLEAGQTVI AVGSPLGSEY ASTVTQGIIS APARTISTSS
     GNQQTVIQTD AAINPGNSGG ALVNSAGQVI GINSMKLAQS SDGTSVEGMA FAIPSNEVVT
     IVNELVKKGK ITRPQLGVRV IALQGIPEGY RSRLKIKSNL KNGIYIAFVS RNGSAANAGI
     KSGDVITKVD GKKVEDVASL HSILYSHKVG DTVNVTVNRN GKDVDMKVKL EGN