HTS1_COCCA
ID HTS1_COCCA Reviewed; 5218 AA.
AC Q01886;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 2.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=HC-toxin synthetase;
DE Short=HTS;
DE EC=6.3.2.-;
GN Name=HTS1;
OS Cochliobolus carbonum (Maize leaf spot fungus) (Bipolaris zeicola).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris.
OX NCBI_TaxID=5017;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=ATCC 90305 / SB111 / 2R15;
RX PubMed=1281482; DOI=10.1016/s0021-9258(18)35714-4;
RA Scott-Craig J.S., Panaccione D.G., Pocard J.-A., Walton J.D.;
RT "The cyclic peptide synthetase catalyzing HC-toxin production in the
RT filamentous fungus Cochliobolus carbonum is encoded by a 15.7-kilobase open
RT reading frame.";
RL J. Biol. Chem. 267:26044-26049(1992).
RN [2]
RP SEQUENCE REVISION TO 3448-3462; 3683 AND 4017.
RC STRAIN=ATCC 90305 / SB111 / 2R15;
RA Scott-Craig J.S., Panaccione D.G., Pocard J.-A., Walton J.D.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RC STRAIN=ATCC 90305 / SB111 / 2R15;
RX PubMed=10671527; DOI=10.1074/jbc.275.7.4906;
RA Cheng Y.-Q., Walton J.D.;
RT "A eukaryotic alanine racemase gene involved in cyclic peptide
RT biosynthesis.";
RL J. Biol. Chem. 275:4906-4911(2000).
CC -!- FUNCTION: Non-ribosomal peptide synthetase, able to activate proline
CC and AEO (2-amino-9,10-epoxi-8-oxodecanoic acid), and epimerize L-Pro.
CC Catalyzes the production of HC-toxin: a cyclic tetrapeptide. Activates
CC and thioesterifies L-Pro, and epimerizes it to D-Pro; also uses D-Ala
CC as a substrate but this is epimerized from L-Ala by TOXG.
CC {ECO:0000269|PubMed:10671527}.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Note=Binds 4 phosphopantetheines covalently.;
CC -!- PATHWAY: Mycotoxin biosynthesis; HC-toxin biosynthesis.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-3 is the initiator.
CC {ECO:0000305}.
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DR EMBL; M98024; AAA33023.2; -; Genomic_DNA.
DR SMR; Q01886; -.
DR UniPathway; UPA00874; -.
DR PHI-base; PHI:12; -.
DR GO; GO:0016881; F:acid-amino acid ligase activity; IDA:UniProtKB.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0009403; P:toxin biosynthetic process; IDA:UniProtKB.
DR Gene3D; 1.10.1200.10; -; 4.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 3.30.559.10; -; 5.
DR Gene3D; 3.40.50.12780; -; 4.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF00668; Condensation; 5.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF47336; SSF47336; 4.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 4.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Ligase; Multifunctional enzyme;
KW Phosphopantetheine; Phosphoprotein; Repeat.
FT CHAIN 1..5218
FT /note="HC-toxin synthetase"
FT /id="PRO_0000193097"
FT DOMAIN 769..843
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 2379..2453
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3532..3608
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 4666..4740
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 249..842
FT /note="Domain 1"
FT REGION 1854..2452
FT /note="Domain 2"
FT REGION 3006..3607
FT /note="Domain 3"
FT REGION 4159..4739
FT /note="Domain 4"
FT MOD_RES 803
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 2414
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3569
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4701
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 5218 AA; 574596 MW; AC947CEA7FF409E2 CRC64;
MTMPHHSSGP AKDSPLCRFP PFPGGNPVFT NIRREKVNFQ LPPPDHALLA AAWAVLLRLY
TGHVKTCFES ATSDQEANLV TYEARDSDTL QTIVLRGACV SSTAEEKAGL RDLNTAVVRT
TVSIDSWTDE MLQDKIAALL QPGKEIVLFQ TPSGCVLVYM QSFMSAMEVK NVSSTLTYIM
SSDPDKTAIR NLSISPRDLA QIMRWNDRKL KSERTNLVYD LFSARAHEQD ANMAIDAWDG
RMSYTELERV SSTWARQLQK QGISQGSWVL FCFEKSRLAV VSMIAILKAG GVCVPIDPRY
PVERIRDIIR TTNATIALVG AGKTAALFKS ADTAVQTIDI TKDIPHGLSD TVVQSNTKID
DPAFGLFTSG STGVPKCIVV THSQICTAVQ AYKDRFGVTS ETRVLQFSSY TFDISIADTF
TALFYGGTLC IPSEEDRMSN LQDYMVSVRP NWAVLTPTVS RFLDPGVVKD FISTLIFTGE
ASREADTVPW IEAGVNLYNV YGPAENTLIT TATRIRKGKS SNIGYGVNTR TWVTDVSGAC
LVPVGSIGEL LIESGHLADK YLNRPDRTEA AFLSDLPWIP NYEGDSVRRG RRFYRTGDLV
RYCDDGSLIC VGRSDTQIKL AGQRVELGDV EAHLQSDPTT SQAAVVFPRS GPLEARLIAL
LVTGNKDGTP HNQQSLPKPA FAQCPPDLVK YATSSLQQRL PSYMVPSVWL GIDFLPMSVS
GKLDRAVLQD QLESLSPSDY AEILGTTGLE VDPGGAASSV ASDSDLRDMN DDSLLLTACS
RVLNLPAGKI SYSQSFIHAG GDSITAMQVS SWMKRFTGKR IGVKDLLVSP SISTAASCIK
SAQDGSRNFV AVRPGQRIPV SPIQKLFFQT AEASKSWNHY HQSFLFRIDQ PIKPQTIEDA
ISLVMQRHPM LQARFERTEE GDWYQYIPID VERRASVEVI GSLSTDDREA AMLRARQSID
LTEGPLIRCQ LFNNNVDEAS RLFFVVIHHA VVDLVSWRII MEELEAHLAT DSTPDRGEAY
QESVPFLAWC QVQAEAVKDI PVDRTVPLIP KIPTADFGYW GLKHDENVYG NTVERKIPLG
HSITEDLLYK CHDSLHTKTI DVLLAAVLVS FRRSFLDRPV PAVFNEGHGR EPGGEDAVDL
SRTVGWFTTI SPVYVPEVSP GDILDVVRRV KDYRWATPNN GFDYFSTKYL TQSGIKLFED
HLPAEILFNY EGRYQAMESE QTVLKPESWH AGEASKDQDP GLRRFCLFEI STAVLPDGQL
HLTCSWNKNM RHQGRIRLWL DTLLPAAIGE IVSSLALASP QLTLSDVELL RLYDYSSLDI
LKKSILSIPA VQTLDDLEGV YPGSPMQDAL FLSQSKSQDG AYEVDFTWRV ATSLQNSQPA
VDIGCLVEAW KDTVALHAAL RTVILESSLP ATGILHQVVL RSHDPDIVIL DVRDVTAAIT
ILDSYPPPTE EGIALIKRPP HRLLICTTIE GSVLIKFQVN HLVFDGMSTD KIIQDLSKAY
TCRHSNKLPD HSESKLHDGT YGNRPTKPPL AEFIRYIRDP QRKQDSINYW KNALRGATTC
SFPPLFDQIT SEKAMPRQSW ASVPIPLCVD SKELSKTLAN LGITMSTMFQ TVWAIVLRIY
SQNGQSVFGY LTSGRDAPVD GIDSAVGNFI AMLVCFFDFD DDGVHTVADM ARKIHNASAN
SISHQACSLA EIQDALGLST STPLFNTAFT YLPKRPTNVK AGEPEHHLCF EELSMSDPTE
FDLTLFVEPT QESNEVSAHL DFKLSYISQA YATSIASTVA HILSELVHDP YRALNTLPIV
SEHDTAIIRS WNDHLFPPAT ECIHETFSRK VVEHPQREAI CSWDGSLTYA ELSDLSQRLS
IHLVSLGIKV GTKIPICFEK SMWTIVTILA VVQAGGVFVL LEPGHPESRL SGIIKQVQAE
LLLCSPATSR MGALQNISTQ MGTEFKIVEL EPEFIRSLPL PPKPNHQPMV GLNDDLYVVF
TSGSTGVPKG AVATHQAYAT GIYEHAVACG MTSLGAPPRS LQFASYSFDA SIGDIFTTLA
VGGCLCIPRE EDRNPAGITT FINRYGVTWA GITPSLALHL DPDAVPTLKA LCVAGEPLSM
SVVTVWSKRL NLINMYGPTE ATVACIANQV TCTTTTVSDI GRGYRATTWV VQPDNHNSLV
PIGAVGELII EGSILCRGYL NDPERTAEVF IRSPSWLHDL RPNSTLYKTG DLVRYSADGK
IIFIGRKDTQ VKMNGQRFEL GEVEHALQLQ LDPSDGPIIV DLLKRTQSGE PDLLIAFLFV
GRANTGTGNS DEIFIATSTS SLSEFSTVIK KLQDAQRAME VLPLFMVPQA YIPIEGGIPL
TAAGKIDRRM LRKLCEPFNR NDLISFTSKA LSTSVKDAET TDTVEDRLAR IWEKVLGVKG
VGRESDFFSS GGNSMAAIAL RAEAQRSGFT LFVADIFTNP RLADMAKLFS HGQSVSPSSS
TLRTKVPISS LQKRSSGLQT AAPVSNGSPV RRCQKENIID CPVAFEYEEG PSDTQLKEAS
RICGISSRSI EDVFPCTPMQ EALVALSLIP GAQASYALHA AFELRPGLDR NRFRSAWEST
VKAQPILRSR IISGSNGSSV VVTSATDSIP QLDVSGLDTF LEQQLQVGFA PGAPLFRLAF
VYSKADDCDY FVISAHHAIY DGWSLNLIWS QVLALYTNGE LPPPGPSFKH FARNLNLVQS
KLDSEDFWRK LLVKPDQESF RFPDVPVGHK PATRCTTNFH FPFSMQSKIG TTANTCINAA
WAITLAQYSS NKTVNFGVTL WGRDFPMIDI EHMTGPTIVT VPRQVNVIPE SSVAEFLQDL
QKSLAVVLPH QHLGLHRIQA LGPIARQACD FSTLLVVNHG SSISWSELEA ADIVPVPLRS
SDLYAYPMVV EVENASSDTL DIRVHSDPDC IEVQLLERLM EQFGHNLQTL CRAASFDPGK
RIAELMDDTA TTHLRTLFSW NSRVKDSPDV AAIAVHKLLE ETAQSQPAES AIVAHDGQLS
YMQMDRCADV LARQIRKTNM ISAQSPFVCI HLLRSATAVV SMLAVLKAGG AFMPVDISQP
RSRLQNLIEE SGAKLVLTLP ESANALATLS GLTKVIPVSL SELVQQITDN TTKKDEYCKS
GDTDPSSPAY LLYTSGTSGK PKGVVMEHRA WSLGFTCHAE YMGFNSCTRI LQFSSLMFDL
SILEIWAVLY AGGCLFIPSD KERVNNLQDF TRINDINTVF LTPSIGKLLN PKDLPNISFA
GFIGEPMTRS LIDAWTLPGR RLVNSYGPTE ACVLVTAREI SPTAPHDKPS SNIGHALGAN
IWVVEPQRTA LVPIGAVGEL CIEAPSLARC YLANPERTEY SFPSTVLDNW QTKKGTRVYR
TGDLVRYASD GTLDFLGRKD GQIKLRGQRI ELGEIEHHIR RLMSDDPRFH EASVQLYNPA
TDPDRDATVD VQMREPYLAG LLVLDLVFTD EVMGIPCTSL TSANTSENLQ TLVTELKKSL
RGVLPHYMVP LHFVAVSRLP TGSSGKLDHA FVRACLRELT APLDGNFPKV EQVLTTNESV
LRQWWGTVLA MDPHSIQRGD DFFSLGGSSI SAMRLVGLAR SSGHKLQHED IFMCPRLADM
AGQISFVQEA SVSPTTSPTI KFDLLDDCEV DEVIDHILPQ LDMNKELIED VYPCTPLQES
LMAATARHGE AYTMIQSITV LASQLAQLKK AMDVVFRDFE VLRTRIALGP SQQALQVVVK
HEELSWESFP SIQSFKDHFY RSLGYGKPLA RLAVITQALD TKQPISHGTR EARTKNSQDT
VMVVVGAHHS IYDAHVLSMI WRRLYREFIG SQADGILEAE TSRSEGVVPF KSYVEKLLRG
KDNDESLLFW KEKLRGVSSS QFPPASWPRV LEHQPSATQT LITKVSLPTS SRKKLGATVA
TVAYAAWALT IAHYTADPDV VFGATLSGRE TMAGSISHPE SIAGPTIITV PLRIIIDFQT
VVSDFLSTLQ KDIVRAAYFG QMMGLNSIAH IDNDCRDACG FKSIIVVQVP DEGENHDGRA
ANPFQMSLES IGHFPAPLVV EVEQSESTDV LIRMAYDPVL VPEKLAHFIS DTFTTTMSNL
SAANPKAKVE SIPALSEAHL AELDVTCPEW ILGKAKDEKI RTESHQCLQD LVCRRAQQSP
NSQAIDSWDG SISYHELDGL SSILAEHLSQ LGVRPEAPVC LLFEKSKWAV VAMIGIIKAG
GCFVPLDPSY PHERLEHIIS ETGSSVIVTS AAYSKLCLSL SVRGIVCDGS VFSSTKKPLP
STADSPPSFS VRPNQAAYIL FTSGSTGKPK GVVMEHHSVC SALIALGKRM GLGPQSRVLQ
FNSYWFDVML LDIFGTLVYG GCLCIPKEEQ RMSNLSGWVQ KFKVNTMLLS TSVSRLMQPA
DTPSLETLCL TGEAVLQSDV DRWAPKLHLI AGYGPTETCI MSVSGELTPS SPANLIGKPV
SCQAWVINPL KETELAPYGA TGELYIQGPT VARGYLHDDV LTSKAFIVDP QWLTGYKTNE
NQWSRRAYKT GDLVFWGPQS NLYYVRRKDS SQVKIRGQRV ELAEIEEVIR QHIPPDVTVC
VDLLSSDDQN TRIILGAVLG IGDRALGGPE DLEVIGYMDD LKSHIIPALE ASLPHHMIPE
AYVPFVQLPT LGSGKLDRKT VRRVAGPLAF SLPQASARHP NQPTVTHTQK LLRQLWCKIL
PQLDESAVNK QDNFLGIGGD SIAAIKLVAL LRQHGISLAV AEIFTRPTLE AMSSLIDEHN
FVVSHAGILS DVTRNTSGVM RQTTNLIAGR HSMAVEKSRE CDNSTLPCTE YQQMFLAGTE
AFTGAHSAQF IFRLPEKIDL DRLQAAFDHC ADWYPNLRTQ IHKDADTGRL LHDISPIGVK
VPWSCHYSDD LNTVLSHDKK FPPGLDGPLH RVTIMRHRDP TESMLVWTLN HAAYDAWSLR
MMLEHITEAY ANPDYEPSYS LGWTAFVLHT ENTKEASRSF WSSYLSDVKP ARLMFNYNLV
SNPRQDRLYE ARINIPKRVL SQATAATVLL AGLTLLVARV CDTRDVILAH LLTGRTLPLA
GIENCPGPTI TKVPLRIPLM DQDLVTLELD SVAKKITAEL MRVMPHEHSG LSAIREFIPQ
AEGTTTSSGK FHAGSVLGRL PLDLVIHPKG GLDLLGKHGL GLQNEGFRLV APPSGGLSME
CALVDDDDDK RSDTISVDVS VLWDQRAATQ EDVIELVHSL QGIFTKRNLA ASICLMYK
