HTT1_ARATH
ID HTT1_ARATH Reviewed; 277 AA.
AC Q8LFW5; F4JNR4; Q9SZQ0;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Protein HEAT-INDUCED TAS1 TARGET 1 {ECO:0000303|PubMed:24728648};
GN Name=HTT1 {ECO:0000303|PubMed:24728648};
GN OrderedLocusNames=At4g29770 {ECO:0000312|Araport:AT4G29770};
GN ORFNames=F27B13.10 {ECO:0000312|EMBL:CAB43652.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP REPRESSED BY SIR480(+)/SIRNA255.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=16061187; DOI=10.1016/j.molcel.2005.06.014;
RA Qi Y., Denli A.M., Hannon G.J.;
RT "Biochemical specialization within Arabidopsis RNA silencing pathways.";
RL Mol. Cell 19:421-428(2005).
RN [6]
RP REPRESSED BY SIRNA255.
RC STRAIN=cv. Columbia;
RX PubMed=18753245; DOI=10.1099/vir.0.2008/002170-0;
RA Meng C., Chen J., Ding S.-W., Peng J., Wong S.-M.;
RT "Hibiscus chlorotic ringspot virus coat protein inhibits trans-acting small
RT interfering RNA biogenesis in Arabidopsis.";
RL J. Gen. Virol. 89:2349-2358(2008).
RN [7]
RP FUNCTION, REPRESSION BY SMALL INTERFERING RNAS, INDUCTION BY HEAT SHOCK,
RP INTERACTION WITH HSP70-14; AT2G33735/HSP40 AND NFYC2, TISSUE SPECIFICITY,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=24728648; DOI=10.1105/tpc.114.124883;
RA Li S., Liu J., Liu Z., Li X., Wu F., He Y.;
RT "HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS
RT TRANSCRIPTION FACTOR A1a-Directed Pathways in Arabidopsis.";
RL Plant Cell 26:1764-1780(2014).
CC -!- FUNCTION: Mediates both basal and acquired thermotolerance via HSFA1s-
CC directed pathways (e.g. HSFA1A, HSFA1B, and HSFA1D). Triggers the
CC expression of HSFA1A and HSFA1B. {ECO:0000269|PubMed:24728648}.
CC -!- SUBUNIT: Interacts with the heat shock proteins HSP70-14 and
CC At2g33735/HSP40, and with NFYC2 in both cytoplasm and nucleus.
CC {ECO:0000269|PubMed:24728648}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24728648}. Nucleus
CC {ECO:0000269|PubMed:24728648}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=HTT1.1 {ECO:0000303|PubMed:24728648};
CC IsoId=Q8LFW5-1; Sequence=Displayed;
CC Name=2; Synonyms=HTT1.2 {ECO:0000303|PubMed:24728648};
CC IsoId=Q8LFW5-2; Sequence=VSP_058823;
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously, including in seedlings,
CC leaves, stems, inflorescences and siliques.
CC {ECO:0000269|PubMed:24728648}.
CC -!- INDUCTION: Target of TAS1 (trans-acting siRNA precursor 1)-derived
CC small interfering RNAs in response to temperature variations, thus
CC reducing both basal and acquired thermotolerance (PubMed:24728648).
CC Repressed by trans-acting small interfering RNA (ta-siRNAs)
CC siR480(+)/siRNA255-mediated transcript cleavage (PubMed:16061187,
CC PubMed:18753245). Highly up-regulated in seedlings exposed to heat
CC shock, with higher levels of isoform 1 than isoform 2. Induced by
CC HSFA1s-mediated (e.g. HSFA1A, HSFA1B, and HSFA1D) promoter activation
CC (PubMed:24728648). {ECO:0000269|PubMed:16061187,
CC ECO:0000269|PubMed:18753245, ECO:0000269|PubMed:24728648}.
CC -!- SIMILARITY: Belongs to the heat induced plant HTT protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB43652.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB79735.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL050352; CAB43652.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL161575; CAB79735.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002687; AEE85673.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85674.1; -; Genomic_DNA.
DR EMBL; BT026041; ABG48397.1; -; mRNA.
DR EMBL; AY084610; AAM61174.1; -; mRNA.
DR PIR; T08538; T08538.
DR RefSeq; NP_001154277.1; NM_001160805.1. [Q8LFW5-2]
DR RefSeq; NP_567833.1; NM_119123.3. [Q8LFW5-1]
DR AlphaFoldDB; Q8LFW5; -.
DR SMR; Q8LFW5; -.
DR STRING; 3702.AT4G29770.2; -.
DR PRIDE; Q8LFW5; -.
DR ProteomicsDB; 232123; -. [Q8LFW5-1]
DR EnsemblPlants; AT4G29770.1; AT4G29770.1; AT4G29770. [Q8LFW5-1]
DR EnsemblPlants; AT4G29770.2; AT4G29770.2; AT4G29770. [Q8LFW5-2]
DR GeneID; 829099; -.
DR Gramene; AT4G29770.1; AT4G29770.1; AT4G29770. [Q8LFW5-1]
DR Gramene; AT4G29770.2; AT4G29770.2; AT4G29770. [Q8LFW5-2]
DR KEGG; ath:AT4G29770; -.
DR Araport; AT4G29770; -.
DR TAIR; locus:2123864; AT4G29770.
DR HOGENOM; CLU_1005918_0_0_1; -.
DR OMA; ECEVEPM; -.
DR OrthoDB; 1037804at2759; -.
DR PRO; PR:Q8LFW5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LFW5; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0010286; P:heat acclimation; IMP:UniProtKB.
DR GO; GO:0009408; P:response to heat; IMP:TAIR.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..277
FT /note="Protein HEAT-INDUCED TAS1 TARGET 1"
FT /id="PRO_0000439259"
FT VAR_SEQ 1
FT /note="M -> MMAISEKGVMAISEKGVMATKIDKNGVLRELRRHFTEFSLRDVDLCL
FT RSSSQM (in isoform 2)"
FT /id="VSP_058823"
SQ SEQUENCE 277 AA; 31130 MW; 5CEF940189BDEB22 CRC64;
MESLLECFAI TDGKCHPDCL KANNEQEDYD ACQSAALVAV SLISSARVIF KIDSKYTEYS
PQYLVDNVGK EEVEGEMDQP SCQYTVGNLL SYLVENVWTK KEVRQREMDQ QRREFTVKDC
FEFAFKKGLP RNGHWAHVGC IFPVPPFACQ IPRVPMKGEV IEAANVSEAL KLGMQQPAAA
RLHLFSPEFD LVGEGIYDGP SGNETRYVGL RDVLMVEAEK IKGETVFTVQ ICYKKKTSFV
KVSTRSMILP LNGDDESQVT EPACLLVDFC IPRFSIN
