HUA2_ARATH
ID HUA2_ARATH Reviewed; 1392 AA.
AC Q9XER9;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 150.
DE RecName: Full=ENHANCER OF AG-4 protein 2;
DE AltName: Full=Protein AERIAL ROSETTE 1;
GN Name=HUA2 {ECO:0000303|PubMed:10198637}; Synonyms=ART1;
GN OrderedLocusNames=At5g23150 {ECO:0000312|Araport:AT5G23150};
GN ORFNames=MKD15, MYJ24.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10198637; DOI=10.1016/s1097-2765(00)80462-1;
RA Chen X., Meyerowitz E.M.;
RT "HUA1 and HUA2 are two members of the floral homeotic AGAMOUS pathway.";
RL Mol. Cell 3:349-360(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=12530963; DOI=10.1016/s1534-5807(02)00399-4;
RA Cheng Y., Kato N., Wang W., Li J., Chen X.;
RT "Two RNA binding proteins, HEN4 and HUA1, act in the processing of AGAMOUS
RT pre-mRNA in Arabidopsis thaliana.";
RL Dev. Cell 4:53-66(2003).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15659097; DOI=10.1111/j.1365-313x.2004.02300.x;
RA Doyle M.R., Bizzell C.M., Keller M.R., Michaels S.D., Song J., Noh Y.-S.,
RA Amasino R.M.;
RT "HUA2 is required for the expression of floral repressors in Arabidopsis
RT thaliana.";
RL Plant J. 41:376-385(2005).
RN [8]
RP VARIANT GLU-525.
RC STRAIN=cv. Sy-0;
RX PubMed=17764945; DOI=10.1016/j.cub.2007.07.059;
RA Wang Q., Sajja U., Rosloski S., Humphrey T., Kim M.C., Bomblies K.,
RA Weigel D., Grbic V.;
RT "HUA2 caused natural variation in shoot morphology of A. thaliana.";
RL Curr. Biol. 17:1513-1519(2007).
RN [9]
RP SUBCELLULAR LOCATION.
RX PubMed=25070081; DOI=10.1111/tpj.12629;
RA Jali S.S., Rosloski S.M., Janakirama P., Steffen J.G., Zhurov V.,
RA Berleth T., Clark R.M., Grbic V.;
RT "A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is
RT essential for development.";
RL Plant J. 80:242-254(2014).
RN [10]
RP FUNCTION.
RX PubMed=25425527; DOI=10.1111/nph.13177;
RA Ilk N., Ding J., Ihnatowicz A., Koornneef M., Reymond M.;
RT "Natural variation for anthocyanin accumulation under high-light and low-
RT temperature stress is attributable to the ENHANCER OF AG-4 2 (HUA2) locus
RT in combination with PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1) and PAP2.";
RL New Phytol. 206:422-435(2015).
CC -!- FUNCTION: Transcription factor that functions as repressor of flowering
CC by enhancing the expression of several genes that delay flowering
CC including FLC, FLM/MAF1, MAF2 and SVP (PubMed:15659097). Also acts in
CC the floral homeotic AGAMOUS (AG) pathway, specifically by processing
CC the AGAMOUS pre-mRNA (PubMed:10198637, PubMed:12530963). Functions in
CC association with HUA1 and HEN4 in AG pre-mRNA processing
CC (PubMed:12530963). Involved in all three aspects of the AG functions,
CC the specification of stamen and carpel identities, the control of
CC floral determinacy, and the spatial restriction of AP1 expression
CC (PubMed:10198637, PubMed:12530963, PubMed:15659097). Acts as
CC transcription regulator that controls anthocyanin accumulation
CC (PubMed:25425527). {ECO:0000269|PubMed:10198637,
CC ECO:0000269|PubMed:12530963, ECO:0000269|PubMed:15659097,
CC ECO:0000269|PubMed:25425527}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25070081}.
CC -!- TISSUE SPECIFICITY: Expressed in the inflorescence meristem, floral
CC primordia, inflorescence stem, and floral pedicels. Also detected in
CC the shoot apical meristem, stems, leaves, embryos, and roots.
CC {ECO:0000269|PubMed:10198637}.
CC -!- DISRUPTION PHENOTYPE: Hua2 mutations enhance the phenotype of mild ag-4
CC and elf4 alleles and also induce early flowering by reducing the
CC expression of several MADS genes that act as floral repressors like
CC FLC, FLM/MAF1, MAF2 and SVP. {ECO:0000269|PubMed:15659097}.
CC -!- MISCELLANEOUS: In cv. Sy-0, a naturally occurring allele K525E, termed
CC ART1, together with active FRI and FLC alleles causes delayed flowering
CC of primary and axillary meristems, resulting in the distinctive Sy-0
CC morphology (PubMed:17764945). {ECO:0000305|PubMed:17764945}.
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DR EMBL; AF116556; AAD31171.1; -; mRNA.
DR EMBL; AB007648; BAB11170.1; -; Genomic_DNA.
DR EMBL; AB006708; BAB11170.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED93127.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69147.1; -; Genomic_DNA.
DR EMBL; AB493755; BAH30593.1; -; mRNA.
DR PIR; T51947; T51947.
DR RefSeq; NP_001330848.1; NM_001343792.1.
DR RefSeq; NP_197706.1; NM_122221.3.
DR AlphaFoldDB; Q9XER9; -.
DR SMR; Q9XER9; -.
DR BioGRID; 17654; 6.
DR STRING; 3702.AT5G23150.1; -.
DR iPTMnet; Q9XER9; -.
DR PaxDb; Q9XER9; -.
DR PRIDE; Q9XER9; -.
DR ProteomicsDB; 230254; -.
DR EnsemblPlants; AT5G23150.1; AT5G23150.1; AT5G23150.
DR EnsemblPlants; AT5G23150.3; AT5G23150.3; AT5G23150.
DR GeneID; 832379; -.
DR Gramene; AT5G23150.1; AT5G23150.1; AT5G23150.
DR Gramene; AT5G23150.3; AT5G23150.3; AT5G23150.
DR KEGG; ath:AT5G23150; -.
DR Araport; AT5G23150; -.
DR TAIR; locus:2178292; AT5G23150.
DR eggNOG; KOG1904; Eukaryota.
DR HOGENOM; CLU_005040_0_0_1; -.
DR InParanoid; Q9XER9; -.
DR OMA; GKHENSP; -.
DR OrthoDB; 143642at2759; -.
DR PhylomeDB; Q9XER9; -.
DR PRO; PR:Q9XER9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9XER9; baseline and differential.
DR Genevisible; Q9XER9; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0043481; P:anthocyanin accumulation in tissues in response to UV light; IMP:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0048497; P:maintenance of floral organ identity; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IMP:UniProtKB.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:TAIR.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd05834; HDGF_related; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR006569; CID_dom.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR035496; HDGF-rel_PWWP.
DR InterPro; IPR000313; PWWP_dom.
DR Pfam; PF04818; CID; 1.
DR Pfam; PF00855; PWWP; 1.
DR SMART; SM00293; PWWP; 1.
DR SMART; SM00582; RPR; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS51391; CID; 1.
DR PROSITE; PS50812; PWWP; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Flowering; mRNA processing;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1392
FT /note="ENHANCER OF AG-4 protein 2"
FT /id="PRO_0000418856"
FT DOMAIN 20..77
FT /note="PWWP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00162"
FT DOMAIN 771..912
FT /note="CID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00724"
FT REGION 184..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 575..613
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 723..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 957..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1014..1356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1369..1392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..198
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 200..217
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..260
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 261..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 305..347
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 387..402
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 404..418
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 588..608
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 723..741
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 742..758
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1058..1131
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1159..1185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1200..1287
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1292..1306
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 525
FT /note="K -> E (in strain: cv. Sy-0; causes late flowering
FT phenotype)"
FT /evidence="ECO:0000269|PubMed:17764945"
SQ SEQUENCE 1392 AA; 151078 MW; D2AC1B0D2AA70F0F CRC64;
MAPGRKRGAS KAKAKGQLVL GDLVLAKVKG FPAWPAKISR PEDWDRAPDP KKYFVQFFGT
EEIAFVAPPD IQAFTSEAKS KLLARCQGKT VKYFAQAVEQ ICTAFEGLQN HKSNALGDED
SLDATEPGLT KAEIVDGTDH IVIESERTDN FNFRVDPCFP KLDENNGEER KAEIRKLDSS
SFLESKVKTT SPVSESLEHS SFDPKIKKED FDKGTDGSAC NEHFGNGQKK LANGKRIKKE
AGGSDRKGED TVHRDKSNNS HVPGGRTASG NSDSKKSKGL LTEKTSSKVS ADKHENSPGI
KVGVSGKKRR LESEQGKLAP RVDESSRAAK KPRCESADNK VKCEIDDGSD STGTVSDIKR
EIVLGLGARG GNFQYDKEAV AYTKRQRQTM EHATSPSFSG SRDKSGKGHL EQKDRSSPVR
NVKAPAAQSL KKRRAVCIYD EDDDEDPKTP LHGKPAIVPQ AASVLTDGPK RANVCHSTST
KAKISAGSTE STEVRKFPLR KHCEDASRVL PSNAENSTNS LPVVKPINEL PPKDVKQILQ
SPKMSPQLVL TNKHVAGQHK VVKSSVKVSG VVMAKKPQSD SCKEAVAGSD KISSSQSQPA
NQRHKSASVG ERLTVVSKAA SRLNDSGSRD MSEDLSAAML DLNREKGSAT FTSAKTPDSA
ASMKDLIAAA QAKRKLAHTQ NSIFGNLNPS FLSISDTQGR SHSPFMVQNA SASAAISMPL
VVQGHHQQGS SPSNHGHQSL SRNQIETDDN EERRLSSGHK SVGGSLSCST EAAISRDAFE
GMLETLSRTR ESIGRATRLA IDCAKYGLAS EVVELLIRKL ESESHFHRKV DLFFLVDSIT
QHSHSQKGIA GASYVPTVQA ALPRLLGAAA PPGTGASDNR RKCLKVLKLW LERKVFPESL
LRRYIDDIRA SGDDATGGFS LRRPSRSERA VDDPIREMEG MLVDEYGSNA TFQLPGFFSS
HNFEDDEEDD DLPTSQKEKS TSAGERVSAL DDLEIHDTSS DKCHRVLEDV DHELEMEDVS
GQRKDVAPSS FCENKTKEQS LDVMEPVAEK STEFNPLPED SPPLPQESPP PLPPLPPSPP
PPSPPLPPSS LPPPPPAALF PPLPPPPSQP PPPPLSPPPS PPPPPPPPSQ SLTTQLSIAS
HHQIPFQPGF PPPTYPLSHQ TYPGSMQQDR SSIFTGDQIV QGPGNSSRGG LVEGAGKPEY
FVQQSSSFSP AGVCSSREPS SFTSSRQLEF GNSDVLFNPE ASSQNHRFQP STPLSQRPMV
RLPSAPSSHF SYPSHIQSQS QHSYTHPYPF PPQRDDARRY RNEEPWRIPS SGHSAENQNG
AWIHGRNSHP GLPRVTDSFF RPPPERPPSG TMNYQPSAAS NLQAVPAIPG HTAPQMLPSR
PDIPTVNCWR PA
