HUB1_YEAST
ID HUB1_YEAST Reviewed; 73 AA.
AC Q6Q546; D6W1K7;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Ubiquitin-like modifier HUB1;
GN Name=HUB1; OrderedLocusNames=YNR032C-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=11923536; DOI=10.1126/science.1069989;
RA Dittmar G.A.G., Wilkinson C.R.M., Jedrzejewski P.T., Finley D.;
RT "Role of a ubiquitin-like modification in polarized morphogenesis.";
RL Science 295:2442-2446(2002).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [6]
RP STRUCTURE BY NMR.
RX PubMed=12943364; DOI=10.1023/a:1024674220425;
RA Ramelot T.A., Cort J.R., Yee A.A., Semesi A., Edwards A.M.,
RA Arrowsmith C.H., Kennedy M.A.;
RT "Solution structure of the yeast ubiquitin-like modifier protein Hub1.";
RL J. Struct. Funct. Genomics 4:25-30(2003).
CC -!- FUNCTION: Forms conjugate with SPH1 and HBT1. Involved in
CC morphogenesis. {ECO:0000269|PubMed:11923536}.
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DR EMBL; Z71648; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY558559; AAS56885.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10573.1; -; Genomic_DNA.
DR RefSeq; NP_014430.1; NM_001184344.1.
DR PDB; 1M94; NMR; -; A=1-73.
DR PDB; 3PLU; X-ray; 1.40 A; A/B=1-73.
DR PDB; 3PLV; X-ray; 1.90 A; A=1-73.
DR PDBsum; 1M94; -.
DR PDBsum; 3PLU; -.
DR PDBsum; 3PLV; -.
DR AlphaFoldDB; Q6Q546; -.
DR BMRB; Q6Q546; -.
DR SMR; Q6Q546; -.
DR BioGRID; 35857; 140.
DR IntAct; Q6Q546; 1.
DR STRING; 4932.YNR032C-A; -.
DR MaxQB; Q6Q546; -.
DR PaxDb; Q6Q546; -.
DR PRIDE; Q6Q546; -.
DR DNASU; 855767; -.
DR EnsemblFungi; YNR032C-A_mRNA; YNR032C-A; YNR032C-A.
DR GeneID; 855767; -.
DR KEGG; sce:YNR032C-A; -.
DR SGD; S000007251; HUB1.
DR VEuPathDB; FungiDB:YNR032C-A; -.
DR eggNOG; KOG3493; Eukaryota.
DR GeneTree; ENSGT00390000001945; -.
DR HOGENOM; CLU_156193_2_0_1; -.
DR InParanoid; Q6Q546; -.
DR OMA; HIKLEDY; -.
DR BioCyc; YEAST:G3O-33397-MON; -.
DR EvolutionaryTrace; Q6Q546; -.
DR PRO; PR:Q6Q546; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; Q6Q546; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0031386; F:protein tag; IDA:SGD.
DR GO; GO:0000753; P:cell morphogenesis involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IPI:SGD.
DR GO; GO:0033120; P:positive regulation of RNA splicing; IMP:SGD.
DR GO; GO:0043687; P:post-translational protein modification; IDA:SGD.
DR GO; GO:0036211; P:protein modification process; IBA:GO_Central.
DR InterPro; IPR039732; Hub1/Ubl5.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR13042; PTHR13042; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..73
FT /note="Ubiquitin-like modifier HUB1"
FT /id="PRO_0000114879"
FT DOMAIN 1..73
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT CONFLICT 12
FT /note="K -> E (in Ref. 3; AAS56885)"
FT /evidence="ECO:0000305"
FT STRAND 1..7
FT /evidence="ECO:0007829|PDB:3PLU"
FT TURN 9..11
FT /evidence="ECO:0007829|PDB:1M94"
FT STRAND 13..19
FT /evidence="ECO:0007829|PDB:3PLU"
FT HELIX 24..35
FT /evidence="ECO:0007829|PDB:3PLU"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:3PLU"
FT STRAND 42..46
FT /evidence="ECO:0007829|PDB:3PLU"
FT HELIX 58..60
FT /evidence="ECO:0007829|PDB:3PLU"
FT STRAND 67..72
FT /evidence="ECO:0007829|PDB:3PLU"
SQ SEQUENCE 73 AA; 8272 MW; 2D5EFB83483F2ABA CRC64;
MIEVVVNDRL GKKVRVKCLA EDSVGDFKKV LSLQIGTQPN KIVLQKGGSV LKDHISLEDY
EVHDQTNLEL YYL
