ID   HUG1_YEAST              Reviewed;          68 AA.
AC   Q6Q5K6; D6VZB5;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=MEC1-mediated checkpoint protein HUG1;
GN   Name=HUG1; OrderedLocusNames=YML058W-A;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=10490641; DOI=10.1128/mcb.19.10.7041;
RA   Basrai M.A., Velculescu V.E., Kinzler K.W., Hieter P.;
RT   "NORF5/HUG1 is a component of the MEC1-mediated checkpoint response to DNA
RT   damage and replication arrest in Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 19:7041-7049(1999).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   INDUCTION.
RX   PubMed=16287844; DOI=10.1128/mcb.25.23.10273-10285.2005;
RA   Carter C.D., Kitchen L.E., Au W.-C., Babic C.M., Basrai M.A.;
RT   "Loss of SOD1 and LYS7 sensitizes Saccharomyces cerevisiae to hydroxyurea
RT   and DNA damage agents and downregulates MEC1 pathway effectors.";
RL   Mol. Cell. Biol. 25:10273-10285(2005).
CC   -!- FUNCTION: Involved in the MEC1-mediated checkpoint response to DNA
CC       damage and replication arrest. {ECO:0000269|PubMed:10490641}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC       {ECO:0000305|PubMed:14562095}.
CC   -!- INDUCTION: By replication arrest and DNA damage. Repressed by CYC8,
CC       RFX1 and TUP1. {ECO:0000269|PubMed:10490641,
CC       ECO:0000269|PubMed:16287844}.
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DR   EMBL; Z46729; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AY557960; AAS56286.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA09839.1; -; Genomic_DNA.
DR   RefSeq; NP_219498.1; NM_001184439.1.
DR   AlphaFoldDB; Q6Q5K6; -.
DR   BioGRID; 35108; 35.
DR   IntAct; Q6Q5K6; 13.
DR   MINT; Q6Q5K6; -.
DR   STRING; 4932.YML058W-A; -.
DR   PaxDb; Q6Q5K6; -.
DR   PRIDE; Q6Q5K6; -.
DR   EnsemblFungi; YML058W-A_mRNA; YML058W-A; YML058W-A.
DR   GeneID; 854944; -.
DR   KEGG; sce:YML058W-A; -.
DR   SGD; S000007472; HUG1.
DR   VEuPathDB; FungiDB:YML058W-A; -.
DR   HOGENOM; CLU_2832613_0_0_1; -.
DR   BioCyc; YEAST:G3O-33020-MON; -.
DR   PRO; PR:Q6Q5K6; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q6Q5K6; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:1990846; F:ribonucleoside-diphosphate reductase inhibitor activity; IGI:SGD.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:SGD.
DR   GO; GO:0000077; P:DNA damage checkpoint signaling; IMP:SGD.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:1905117; P:regulation of ribonucleoside-diphosphate reductase activity; IGI:SGD.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA damage; DNA repair; Nucleus; Reference proteome.
FT   CHAIN           1..68
FT                   /note="MEC1-mediated checkpoint protein HUG1"
FT                   /id="PRO_0000240383"
SQ   SEQUENCE   68 AA;  7514 MW;  5A6482CA7D053293 CRC64;
     MTMDQGLNPK QFFLDDVVLQ DTLCSMSNRV NKSVKTGYLF PKDHVPSANI IAVERRGGLS
     DIGKNTSN