ID   HUGA_POLAN              Reviewed;         367 AA.
AC   Q9U6V9;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Hyaluronidase;
DE            Short=Hya;
DE            EC=3.2.1.35;
DE   AltName: Full=Hyaluronoglucosaminidase;
DE   AltName: Allergen=Pol a 2;
DE   Flags: Precursor; Fragment;
OS   Polistes annularis (Paper wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Polistinae; Polistini; Polistes.
OX   NCBI_TaxID=27505;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   King T.P., Lu G.;
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play a role in reproduction. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC         glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC   -!- ALLERGEN: Causes an allergic reaction in human.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family. {ECO:0000305}.
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DR   EMBL; AF174528; AAD52616.1; -; mRNA.
DR   AlphaFoldDB; Q9U6V9; -.
DR   SMR; Q9U6V9; -.
DR   Allergome; 3431; Pol a 2.0101.
DR   Allergome; 584; Pol a 2.
DR   CAZy; GH56; Glycoside Hydrolase Family 56.
DR   GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR018155; Hyaluronidase.
DR   InterPro; IPR001329; Venom_Hyaluronidase.
DR   PANTHER; PTHR11769; PTHR11769; 1.
DR   Pfam; PF01630; Glyco_hydro_56; 1.
DR   PIRSF; PIRSF038193; Hyaluronidase; 1.
DR   PRINTS; PR00846; GLHYDRLASE56.
DR   PRINTS; PR00847; HYALURONDASE.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   1: Evidence at protein level;
KW   Allergen; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Signal;
KW   Zymogen.
FT   SIGNAL          <1..?
FT   PROPEP          ?..29
FT                   /id="PRO_0000012107"
FT   CHAIN           30..367
FT                   /note="Hyaluronidase"
FT                   /id="PRO_0000012108"
FT   ACT_SITE        138
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        108
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        354
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        48..337
FT                   /evidence="ECO:0000250"
FT   DISULFID        214..226
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   367 AA;  43020 MW;  8127056216957562 CRC64;
     YVSLSPDSVF NIITDDISHQ ILSRSNCERS KRPKRVFSIY WNVPTFMCHQ YGMNFDEVTD
     FNIKHNSKDN FRGETISIYY DPGKFPALMP LKNGNYEERN GGVPQRGNIT IHLQQFNEDL
     DKMTPDKNFG GIGVIDFERW KPIFRQNWGN TEIHKKYSIE LVRKEHPKWS ESMIEAEATK
     KFEKYARYFM EETLKLAKKT RKRAKWGYYG FPYCYNVTPN NPGPDCDAKA TIENDRLSWM
     YNNQEILFPS VYVRHEQKPE ERVYLVQGRI KEAVRISNNL EHSPSVLAYW WYVYQDKMDI
     YLSETDVEKT FQEIVTNGGD GIIIWGSSSD VNSLSKCKRL REYLLNTLGP FAVNVTETVN
     GRSSLNF