HUNK_MOUSE
ID HUNK_MOUSE Reviewed; 714 AA.
AC O88866;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Hormonally up-regulated neu tumor-associated kinase;
DE EC=2.7.11.1;
DE AltName: Full=Serine/threonine-protein kinase MAK-V;
GN Name=Hunk; Synonyms=Makv;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=A/Sn; TISSUE=Mammary gland;
RX PubMed=9273061;
RA Korobko I.V., Kabishev A.A., Kiselev S.L.;
RT "Identification of the new protein kinase specifically transcribed in mouse
RT tumors with high metastatic potential.";
RL Dokl. Akad. Nauk 354:554-556(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=FVB/NJ; TISSUE=Mammary gland;
RX PubMed=10662544; DOI=10.1006/geno.1999.6078;
RA Gardner H.P., Wertheim G.B.W., Ha S.I., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Marquis S.T., Chodosh L.A.;
RT "Cloning and characterization of Hunk, a novel mammalian SNF1-related
RT protein kinase.";
RL Genomics 63:46-59(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr
CC protein kinase family. SNF1 subfamily. {ECO:0000305}.
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DR EMBL; AF055919; AAC61489.1; -; mRNA.
DR EMBL; AF167987; AAF35282.1; -; mRNA.
DR CCDS; CCDS28315.1; -.
DR RefSeq; NP_056570.1; NM_015755.2.
DR AlphaFoldDB; O88866; -.
DR SMR; O88866; -.
DR BioGRID; 205017; 1.
DR STRING; 10090.ENSMUSP00000068007; -.
DR iPTMnet; O88866; -.
DR PhosphoSitePlus; O88866; -.
DR MaxQB; O88866; -.
DR PaxDb; O88866; -.
DR PRIDE; O88866; -.
DR ProteomicsDB; 273203; -.
DR Antibodypedia; 6820; 350 antibodies from 31 providers.
DR DNASU; 26559; -.
DR Ensembl; ENSMUST00000065856; ENSMUSP00000068007; ENSMUSG00000053414.
DR GeneID; 26559; -.
DR KEGG; mmu:26559; -.
DR UCSC; uc007zwi.1; mouse.
DR CTD; 30811; -.
DR MGI; MGI:1347352; Hunk.
DR VEuPathDB; HostDB:ENSMUSG00000053414; -.
DR eggNOG; KOG0583; Eukaryota.
DR GeneTree; ENSGT00940000161070; -.
DR HOGENOM; CLU_017161_0_0_1; -.
DR InParanoid; O88866; -.
DR OMA; CLISQIQ; -.
DR OrthoDB; 1127668at2759; -.
DR PhylomeDB; O88866; -.
DR TreeFam; TF352373; -.
DR BRENDA; 2.7.11.1; 3474.
DR BioGRID-ORCS; 26559; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Hunk; mouse.
DR PRO; PR:O88866; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; O88866; protein.
DR Bgee; ENSMUSG00000053414; Expressed in epithelium of cochlear duct and 222 other tissues.
DR ExpressionAtlas; O88866; baseline and differential.
DR Genevisible; O88866; MM.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IDA:MGI.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IDA:MGI.
DR CDD; cd14070; STKc_HUNK; 1.
DR InterPro; IPR034671; Hunk.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..714
FT /note="Hormonally up-regulated neu tumor-associated kinase"
FT /id="PRO_0000086005"
FT DOMAIN 62..320
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 624..658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 674..714
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 682..702
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 186
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 68..76
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 91
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT CONFLICT 697
FT /note="T -> I (in Ref. 2; AAF35282)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 714 AA; 79603 MW; D35A53E7A8D9BD1F CRC64;
MPAAAGDGLL GEPAAPGGDG GAEDTTRPAA ACEGSFLPAW VSGVSRERLR DFQHHKRVGN
YLIGSRKLGE GSFAKVREGL HVLTGEKVAI KVIDKKRAKK DTYVTKNLRR EGQIQQMIRH
PNITQLLDIL ETENSYYLVM ELCPGGNLMH KIYEKKRLDE AEARRYIRQL ISAVEHLHRA
GVVHRDLKIE NLLLDEDNNI KLIDFGLSNC AGILGYSDPF STQCGSPAYA APELLARKKY
GPKIDVWSIG VNMYAMLTGT LPFTVEPFSL RALYQKMVDK AMNPLPTQLS TGAVNFLRSL
LEPDPVKRPN IQQALANRWL NENYTGKVPC NVTYPNRISL EDLSPSVVLH MTEKLGYKNS
DVINTVLSNR ACHILAIYFL LNKKLERYLS GKSDIQDSIC YKTQLYQIEK CRATKEPYEA
SLDTWTRDFE FHAVQDKKPK EQEKRGDFLH RPFSKKLDKN LPSHKQPSPS LITQLQSTKA
LLKDRKASKS GFPDKDSFVC RNLFRKTSDS NCVASSSMEF IPVPPPRTPR IVKKLEPHQP
GPGSASILPK EEPLLLDMVR SFESVDREDH IELLSPSHHY RILSSPVSLA RRNSSERTLS
QGLLSGSTSP LQTPLHSTLV SFAHEEKNSP PKEEGVCSPP PVPSNGLLQP LGSPNCVKSR
GRFPMMGIGQ MLRKRHQSLQ PSSERSLDAS MSPLQPTAPS SLSFDMADGV KGQC
