ID   HUPD_BRADU              Reviewed;         192 AA.
AC   Q45251; Q45249;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Hydrogenase expression/formation protein HupD;
GN   Name=hupD; OrderedLocusNames=bll6939;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8230232; DOI=10.1006/jmbi.1993.1605;
RA   van Soom C., Browaeys J., Verreth C., Vanderleyden J.;
RT   "Nucleotide sequence analysis of four genes, hupC, hupD, hupF and hupG,
RT   downstream of the hydrogenase structural genes in Bradyrhizobium
RT   japonicum.";
RL   J. Mol. Biol. 234:508-512(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=8163174; DOI=10.1016/0378-1119(94)90126-0;
RA   Fu C., Maier R.J.;
RT   "Sequence and characterization of three genes within the hydrogenase gene
RT   cluster of Bradyrhizobium japonicum.";
RL   Gene 141:47-52(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Not known. Could be involved in the processing of
CC       hydrogenase.
CC   -!- SIMILARITY: Belongs to the peptidase A31 family. {ECO:0000305}.
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DR   EMBL; Z21948; CAA79944.1; -; Genomic_DNA.
DR   EMBL; L24446; AAD13469.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC52204.1; -; Genomic_DNA.
DR   PIR; S39401; S39401.
DR   RefSeq; NP_773579.1; NC_004463.1.
DR   RefSeq; WP_011089677.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q45251; -.
DR   SMR; Q45251; -.
DR   STRING; 224911.27355220; -.
DR   MEROPS; A31.002; -.
DR   EnsemblBacteria; BAC52204; BAC52204; BAC52204.
DR   GeneID; 64026695; -.
DR   KEGG; bja:bll6939; -.
DR   PATRIC; fig|224911.44.peg.6973; -.
DR   eggNOG; COG0680; Bacteria.
DR   HOGENOM; CLU_099037_0_1_5; -.
DR   InParanoid; Q45251; -.
DR   OMA; MGAKKMS; -.
DR   PhylomeDB; Q45251; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016485; P:protein processing; IEA:InterPro.
DR   Gene3D; 3.40.50.1450; -; 1.
DR   InterPro; IPR004419; Pept_A31_hyd_express.
DR   InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR   InterPro; IPR000671; Peptidase_A31.
DR   PANTHER; PTHR30302; PTHR30302; 1.
DR   Pfam; PF01750; HycI; 1.
DR   PRINTS; PR00446; HYDRGNUPTAKE.
DR   SUPFAM; SSF53163; SSF53163; 1.
DR   TIGRFAMs; TIGR00140; hupD; 1.
DR   TIGRFAMs; TIGR00072; hydrog_prot; 1.
PE   3: Inferred from homology;
KW   Aspartyl protease; Hydrolase; Metal-binding; Nickel; Protease;
KW   Reference proteome.
FT   CHAIN           1..192
FT                   /note="Hydrogenase expression/formation protein HupD"
FT                   /id="PRO_0000201938"
FT   BINDING         23
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         69
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         100
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        149
FT                   /note="S -> A (in Ref. 1; CAA79944)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        171..175
FT                   /note="LAESE -> IWRSPS (in Ref. 1; CAA79944)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   192 AA;  21248 MW;  C643C16250EE2157 CRC64;
     MPTSSQDNRI LVLGIGNILW ADEGFGVRAV EEFHRRYAVP DNVTILDGGT QGLYLVNYLE
     EADRLIVFDA IDYGLEPGRL KLVRDDEVPR FTGAKKMSLH QTGFQEVISA ADLLGRCPKH
     LVLIGCQPLD LEDWGGPLTP PVRDQIAPSI DLACQVLAEW GVTVSRRSAP LAESERLLAN
     DIDHANYEMR PA