ID   HUPD_RHILV              Reviewed;         202 AA.
AC   P27649; Q52829;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Hydrogenase expression/formation protein HupD;
GN   Name=hupD;
OS   Rhizobium leguminosarum bv. viciae.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=387;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UPM791;
RX   PubMed=1597428; DOI=10.1128/jb.174.12.4130-4139.1992;
RA   Hidalgo E., Palacios J.M., Murillo J., Ruiz-Argueso T.;
RT   "Nucleotide sequence and characterization of four additional genes of the
RT   hydrogenase structural operon from Rhizobium leguminosarum bv. viciae.";
RL   J. Bacteriol. 174:4130-4139(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B10;
RA   Brito B., Palacios J.M., Imperial J., Ruiz-Argueso T., Yang W.C.,
RA   Bisseling T., Schmitt H., Kerl V., Bauer T., Kokotek W., Lotz W.;
RT   "Organization of the hup-region and its differential transcription in non-
RT   symbiotic and symbiotic cells of Rhizobium leguminosarum bv. viciae B10.";
RL   Mol. Plant Microbe Interact. 8:235-240(1997).
CC   -!- FUNCTION: Not known. Could be involved in the processing of
CC       hydrogenase.
CC   -!- SIMILARITY: Belongs to the peptidase A31 family. {ECO:0000305}.
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DR   EMBL; X52974; CAA37151.1; -; Genomic_DNA.
DR   EMBL; Z36981; CAA85433.1; -; Genomic_DNA.
DR   PIR; B41892; B41892.
DR   RefSeq; WP_018517048.1; NZ_WIDT01000003.1.
DR   AlphaFoldDB; P27649; -.
DR   SMR; P27649; -.
DR   MEROPS; A31.002; -.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016485; P:protein processing; IEA:InterPro.
DR   Gene3D; 3.40.50.1450; -; 1.
DR   InterPro; IPR004419; Pept_A31_hyd_express.
DR   InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR   InterPro; IPR000671; Peptidase_A31.
DR   PANTHER; PTHR30302; PTHR30302; 1.
DR   Pfam; PF01750; HycI; 1.
DR   PRINTS; PR00446; HYDRGNUPTAKE.
DR   SUPFAM; SSF53163; SSF53163; 1.
DR   TIGRFAMs; TIGR00140; hupD; 1.
DR   TIGRFAMs; TIGR00072; hydrog_prot; 1.
PE   3: Inferred from homology;
KW   Aspartyl protease; Hydrolase; Metal-binding; Nickel; Protease.
FT   CHAIN           1..202
FT                   /note="Hydrogenase expression/formation protein HupD"
FT                   /id="PRO_0000201939"
FT   BINDING         28
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         74
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         105
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        72
FT                   /note="V -> G (in Ref. 1; CAA37151)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   202 AA;  22089 MW;  BD7E5A2053D38942 CRC64;
     MTIPYPLGPP PAPRILVLGI GNILWADEGF GVRAVEAFHK AYELSDNVTI LDGGTQGLYL
     VQFVNEHDRL IVFDAIDYGL EPGTMKVVED DEVPKFTGAK KMSLHQTGFQ EVLSAADFMG
     HYPERLTLIG CQPLDLEDWG GPLTAPVRGV IPAAIETAVR VLRSWGVAVT ARPEGAAVPP
     LLEHDIDFER YERRAEPAAL NC