ID   HUPD_RHOCA              Reviewed;         210 AA.
AC   Q03004;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Hydrogenase expression/formation protein HupD;
GN   Name=hupD;
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=8497190; DOI=10.1111/j.1365-2958.1993.tb01199.x;
RA   Colbeau A., Richaud P., Toussaint B., Caballero F.J., Elster C.,
RA   Delphin C., Smith R.L., Chabert J., Vignais P.M.;
RT   "Organization of the genes necessary for hydrogenase expression in
RT   Rhodobacter capsulatus. Sequence analysis and identification of two hyp
RT   regulatory mutants.";
RL   Mol. Microbiol. 8:15-29(1993).
CC   -!- FUNCTION: Not known. Could be involved in the processing of
CC       hydrogenase.
CC   -!- SIMILARITY: Belongs to the peptidase A31 family. {ECO:0000305}.
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DR   EMBL; Z15089; CAA78798.1; -; Genomic_DNA.
DR   PIR; S32941; S32941.
DR   RefSeq; WP_013066514.1; NZ_VIBE01000017.1.
DR   AlphaFoldDB; Q03004; -.
DR   SMR; Q03004; -.
DR   MEROPS; A31.002; -.
DR   GeneID; 31489712; -.
DR   OMA; MGAKKMS; -.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016485; P:protein processing; IEA:InterPro.
DR   Gene3D; 3.40.50.1450; -; 1.
DR   InterPro; IPR004419; Pept_A31_hyd_express.
DR   InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR   InterPro; IPR000671; Peptidase_A31.
DR   PANTHER; PTHR30302; PTHR30302; 1.
DR   Pfam; PF01750; HycI; 1.
DR   PRINTS; PR00446; HYDRGNUPTAKE.
DR   SUPFAM; SSF53163; SSF53163; 1.
DR   TIGRFAMs; TIGR00140; hupD; 1.
DR   TIGRFAMs; TIGR00072; hydrog_prot; 1.
PE   3: Inferred from homology;
KW   Aspartyl protease; Hydrolase; Metal-binding; Nickel; Protease.
FT   CHAIN           1..210
FT                   /note="Hydrogenase expression/formation protein HupD"
FT                   /id="PRO_0000201940"
FT   BINDING         22
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         99
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   210 AA;  22833 MW;  D3AB979385E8FD5C CRC64;
     MPAFKPERVL VLGIGNVLWA DEGFGVRCVE RMAETHALPA NVRLLDGGTQ GLYLLPFLEE
     AEALIVFDAV DFGFTPGTLV TMRDDDVPAF MGAKKMSLHQ TGFQDVIATA QLMGYCPSRM
     TLIGCQPVEL EDYGGSLRPA VAGQIDFAIA EAVRELRAWG IEVTKGATIS NDLVDPSLAR
     DAYERGRPSE DEACRIGDHR FFPSAAKVRA