ID   HUPE_RHILV              Reviewed;         191 AA.
AC   P27650;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Protein HupE;
DE   Flags: Precursor;
GN   Name=hupE;
OS   Rhizobium leguminosarum bv. viciae.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=387;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UPM791;
RX   PubMed=1597428; DOI=10.1128/jb.174.12.4130-4139.1992;
RA   Hidalgo E., Palacios J.M., Murillo J., Ruiz-Argueso T.;
RT   "Nucleotide sequence and characterization of four additional genes of the
RT   hydrogenase structural operon from Rhizobium leguminosarum bv. viciae.";
RL   J. Bacteriol. 174:4130-4139(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B10;
RA   Brito B., Palacios J.M., Imperial J., Ruiz-Argueso T., Yang W.C.,
RA   Bisseling T., Schmitt H., Kerl V., Bauer T., Kokotek W., Lotz W.;
RT   "Organization of the hup-region and its differential transcription in non-
RT   symbiotic and symbiotic cells of Rhizobium leguminosarum bv. viciae B10.";
RL   Mol. Plant Microbe Interact. 8:235-240(1997).
CC   -!- FUNCTION: Needed for full hydrogenase activity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
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DR   EMBL; X52974; CAA37152.1; -; Genomic_DNA.
DR   EMBL; Z36981; CAA85434.1; -; Genomic_DNA.
DR   PIR; C41892; C41892.
DR   RefSeq; WP_018517049.1; NZ_WIEJ01000010.1.
DR   AlphaFoldDB; P27650; -.
DR   TCDB; 2.A.99.1.1; the 6tms ni(2+) uptake transporter (hupe-urej) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007038; HupE_UreJ.
DR   Pfam; PF04955; HupE_UreJ; 1.
DR   PIRSF; PIRSF016919; HupE_UreJ; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..191
FT                   /note="Protein HupE"
FT                   /id="PRO_0000013608"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   191 AA;  19022 MW;  5DC0FB569ED12299 CRC64;
     MKYSKITTTL AALALPSIAH AHVGLHADGT LAGLNHPFSG LDHILAMVAV GFWASTLGGK
     AVWIVPSAFV IVMAGGGVLG IEGIALPMVE TAIALTVAML GLLVAFEVKI PTPVAAIVVG
     ICALFHGHVH GIELPTMSNA TGYVAGFLAA TVILHVLGIG LASLRFGKAG QVVARVAGGA
     VALAGAALLV G