ID   HUPK_RHILV              Reviewed;         370 AA.
AC   P28153;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Hydrogenase expression/formation protein HupK;
GN   Name=hupK;
OS   Rhizobium leguminosarum bv. viciae.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=387;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=128c53;
RX   PubMed=1469733; DOI=10.1016/0022-2836(92)90886-o;
RA   Rey L., Hidalgo E., Palacios J.M., Ruiz-Argueso T.;
RT   "Nucleotide sequence and organization of an H2-uptake gene cluster from
RT   Rhizobium leguminosarum bv. viciae containing a rubredoxin-like gene and
RT   four additional open reading frames.";
RL   J. Mol. Biol. 228:998-1002(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7934943; DOI=10.1111/j.1365-2958.1993.tb01260.x;
RA   Imperial J., Rey L., Ruiz-Argueso T.;
RT   "HupK, a hydrogenase-ancillary protein from Rhizobium leguminosarum, shares
RT   structural motifs with the large subunit of NiFe hydrogenases and could be
RT   a scaffolding protein for hydrogenase metal cofactor assembly.";
RL   Mol. Microbiol. 9:1305-1306(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B10;
RA   Brito B., Palacios J.M., Imperial J., Ruiz-Argueso T., Yang W.C.,
RA   Bisseling T., Schmitt H., Kerl V., Bauer T., Kokotek W., Lotz W.;
RT   "Organization of the hup-region and its differential transcription in non-
RT   symbiotic and symbiotic cells of Rhizobium leguminosarum bv. viciae B10.";
RL   Mol. Plant Microbe Interact. 8:235-240(1997).
CC   -!- SIMILARITY: Belongs to the HupK family. {ECO:0000305}.
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DR   EMBL; X52974; CAA37158.1; -; Genomic_DNA.
DR   EMBL; Z36981; CAA85440.1; -; Genomic_DNA.
DR   PIR; S27344; S27344.
DR   RefSeq; WP_018517055.1; NZ_WIEG01000075.1.
DR   AlphaFoldDB; P28153; -.
DR   SMR; P28153; -.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 2.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   SUPFAM; SSF56762; SSF56762; 1.
PE   3: Inferred from homology;
FT   CHAIN           1..370
FT                   /note="Hydrogenase expression/formation protein HupK"
FT                   /id="PRO_0000201432"
FT   CONFLICT        240
FT                   /note="A -> R (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   370 AA;  38751 MW;  ED17BC76EB2F28C9 CRC64;
     MTFLLGAGTI GIDVTVSRAL ACSVAVKANR PRGLTRMFVG RQPEEAPVLA GQVFSLCGFA
     QSVAARLAVL AAADLAMNDE ERLGASAGLL AERIFETLRA LILQWPTPLP ERFAADAGRH
     LREALAASLA IISHAKAGRT SRPRLAAAAE RLSAAATALG IPGRGDTPLP ETACAAILRD
     VEDDHVFAGR RPDPLTISDD AEVVARLRDE AGYASLPHLS GRIAETGAYA RSASAGLPEA
     PHLARRLRAR IGDVRISLTQ LTALARTGDF DCASLACSGP TPGAGGYGAV ECARGRLYHQ
     IEIGGDGRLA AYRILAPTEW NFHPAGPFVE TLLSSPVGAD EAAVRSISRL AVLFDPCVAF
     EINVREAADA