ID   HUPM_AZOCH              Reviewed;         209 AA.
AC   Q43954;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Hydrogenase expression/formation protein HupM;
GN   Name=hupM;
OS   Azotobacter chroococcum mcd 1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7966281; DOI=10.1016/0022-2836(94)90029-9;
RA   Du L., Tibelius K.H., Souza E.M., Garg R.P., Yates M.G.;
RT   "Sequences, organization and analysis of the hupZMNOQRTV genes from the
RT   Azotobacter chroococcum hydrogenase gene cluster.";
RL   J. Mol. Biol. 243:549-557(1994).
CC   -!- FUNCTION: Not known. Could be involved in the processing of
CC       hydrogenase.
CC   -!- SIMILARITY: Belongs to the peptidase A31 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L25315; AAA64448.1; -; Genomic_DNA.
DR   PIR; S53657; S53657.
DR   AlphaFoldDB; Q43954; -.
DR   SMR; Q43954; -.
DR   MEROPS; A31.002; -.
DR   PRIDE; Q43954; -.
DR   GeneID; 61933040; -.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016485; P:protein processing; IEA:InterPro.
DR   Gene3D; 3.40.50.1450; -; 1.
DR   InterPro; IPR004419; Pept_A31_hyd_express.
DR   InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR   InterPro; IPR000671; Peptidase_A31.
DR   PANTHER; PTHR30302; PTHR30302; 1.
DR   Pfam; PF01750; HycI; 1.
DR   PRINTS; PR00446; HYDRGNUPTAKE.
DR   SUPFAM; SSF53163; SSF53163; 1.
DR   TIGRFAMs; TIGR00140; hupD; 1.
DR   TIGRFAMs; TIGR00072; hydrog_prot; 1.
PE   3: Inferred from homology;
KW   Aspartyl protease; Hydrolase; Metal-binding; Nickel; Protease.
FT   CHAIN           1..209
FT                   /note="Hydrogenase expression/formation protein HupM"
FT                   /id="PRO_0000201936"
FT   BINDING         21
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         67
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         98
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   209 AA;  22833 MW;  8D6B5E12B4C907E1 CRC64;
     MTGSSPNILI LGIGNLLWAD EGFGVRCVEL LNERYRFPDG VRLMDGGTQG IYLVQHVQQA
     DCLIVFDAVD YGLAPGTLKV VRDDEVPRFM GAKRMSLHQT GFQDVLALAA FTGAYPRELL
     LVGVQPAELE DFGGSLREPV RAQLEPALAI ALAFLAERGV LATPREGDAE QLAPAQLALG
     RYEAERPAEE LAYRHGDIRF IAQPGREDD