ID   HUPN_BRADU              Reviewed;         381 AA.
AC   Q45247;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Hydrogenase nickel incorporation protein HupN;
GN   Name=hupN; OrderedLocusNames=bll6949;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=8197192; DOI=10.1073/pnas.91.11.5099;
RA   Fu C., Javedan S., Moshiri F., Maier R.J.;
RT   "Bacterial genes involved in incorporation of nickel into a hydrogenase
RT   enzyme.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:5099-5103(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Involved in nickel incorporation/metabolism into the
CC       hydrogenase apoprotein.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC       {ECO:0000305}.
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DR   EMBL; L24432; AAC36884.1; -; Unassigned_DNA.
DR   EMBL; BA000040; BAC52214.1; -; Genomic_DNA.
DR   RefSeq; NP_773589.1; NC_004463.1.
DR   AlphaFoldDB; Q45247; -.
DR   STRING; 224911.27355230; -.
DR   TCDB; 2.A.52.1.6; the ni(2+)-co(2+) transporter (nicot) family.
DR   EnsemblBacteria; BAC52214; BAC52214; BAC52214.
DR   KEGG; bja:bll6949; -.
DR   PATRIC; fig|224911.5.peg.7120; -.
DR   eggNOG; COG3376; Bacteria.
DR   HOGENOM; CLU_036094_2_0_5; -.
DR   InParanoid; Q45247; -.
DR   OMA; VSKSWHI; -.
DR   PhylomeDB; Q45247; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR004688; Ni/Co_transpt.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   PANTHER; PTHR31611; PTHR31611; 1.
DR   Pfam; PF03824; NicO; 1.
DR   TIGRFAMs; TIGR00802; nico; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Nickel; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..381
FT                   /note="Hydrogenase nickel incorporation protein HupN"
FT                   /id="PRO_0000194005"
FT   TOPO_DOM        1..39
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        61..63
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        85..110
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        132..149
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        171..215
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        237..243
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        265..292
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        314..333
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        355..381
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   381 AA;  40238 MW;  29EF48D8CD10769C CRC64;
     MLPFSMTGLE KDHTRGVLIL ANAHRRSERS RTASCAGPAV LFGGLITANI VAWAWAFALF
     ADRPVVMATA LLAWVFGLRH AVDADHIAAI DNVVRSLMQT GGTPRSAGLY FALGHSSVVV
     VATMLLALGV VSLGGDGLLK EIGSFIGASV SALFLLVIAA INLAIFASLW RTFRKAREQG
     IRDAAGLDAL LAHRGILVRL LGPMFRLVTK PWHMYPLGFL FGLGFDTATE IGLLSISASE
     AARGASLADV MVFPALFAAG MALVDTADST LMVSAYRWAF VDPMRKLWYN LTITGASVAV
     ALFIGGIEAL GLIGNRLDLS GGVWTLIDAL NESLANVGLA VIALFAIAWL LSIVLYRRLI
     AGSSGLADTE VLECADATEA V