ID   HUR_KITAU               Reviewed;         340 AA.
AC   Q00923;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Hydroxyurea phosphotransferase;
DE            EC=2.7.-.-;
DE   AltName: Full=Hydroxyurea resistance protein;
GN   Name=hur;
OS   Kitasatospora aureofaciens (Streptomyces aureofaciens).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Kitasatospora.
OX   NCBI_TaxID=1894;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10762 / DSM 40127 / CCM 3239 / JCM 4008 / LMG 5968 / NBRC 12843
RC   / NCIMB 8234 / A-377;
RX   PubMed=1316866; DOI=10.1016/0378-1119(92)90719-6;
RA   Kormanec J., Farkasovsky M., Potuchkova L., Godar S.;
RT   "A gene (hur) from Streptomyces aureofaciens, conferring resistance to
RT   hydroxyurea, is related to genes encoding streptomycin
RT   phosphotransferase.";
RL   Gene 114:133-137(1992).
CC   -!- FUNCTION: Potential phosphotransferase that inactivates hydroxyurea by
CC       phosphorylation of the hydroxy group in the hydroxylamine moiety.
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M81739; AAA26767.1; -; Genomic_DNA.
DR   PIR; JC1125; JC1125.
DR   AlphaFoldDB; Q00923; -.
DR   SMR; Q00923; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   GO; GO:0019748; P:secondary metabolic process; IEA:InterPro.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR006748; NH2Glyco/OHUrea_AB-resist_kin.
DR   Pfam; PF04655; APH_6_hur; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Transferase.
FT   CHAIN           1..340
FT                   /note="Hydroxyurea phosphotransferase"
FT                   /id="PRO_0000204819"
FT   ACT_SITE        240
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   340 AA;  36049 MW;  7D16C52D350BB3E9 CRC64;
     MRGTPAVLVD GKPVGAKDGS MFDAAGFART LKGRAWLRRV GAVVEIPAEL VEVQLRYGGA
     AGREFVEGLP GRVAEFLERW GLRVAGPAMH GMTALVLPVV RADGTEAALK LLVPDEESAG
     EPVALRAWDG RGCVRLLEWD GPTGTLLLER LDAGRHLSGL VERDARAAVT VVAGLLARLT
     SVRAPEGLRG LGEVAAGLLA GVPEAAGRLA DAGERRLLRD CAAALAEVAA EPGDRLLHWD
     LHFGNVLAGE REPWLAIDPK PLAGDPGFEL LPALVNGFRA GEVGWRFDLL TGVVGLDRER
     ARAWTLGRVV QNCLWEVEDG EVALPEREVA VAEVLLGRAG