ID   HUS1_SCHPO              Reviewed;         287 AA.
AC   P78955;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 140.
DE   RecName: Full=Checkpoint protein hus1;
GN   Name=hus1; ORFNames=SPAC20G4.04c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX   PubMed=9180692; DOI=10.1007/pl00008606;
RA   Kostrub C.F., Al-Khodairy F., Ghazizadeh H., Carr A.M., Enoch T.;
RT   "Molecular analysis of hus1+, a fission yeast gene required for S-M and DNA
RT   damage checkpoints.";
RL   Mol. Gen. Genet. 254:389-399(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   INTERACTION WITH MUG40.
RX   PubMed=12514100; DOI=10.1101/gad.1043203;
RA   Kai M., Wang T.S.-F.;
RT   "Checkpoint activation regulates mutagenic translesion synthesis.";
RL   Genes Dev. 17:64-76(2003).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: Essential in controlling the S-M checkpoint that couples
CC       mitosis to the completion of DNA replication. It is also required for
CC       the response to DNA damage.
CC   -!- SUBUNIT: Interacts with mug40. {ECO:0000269|PubMed:12514100}.
CC   -!- INTERACTION:
CC       P78955; Q10159: myh1; NbExp=4; IntAct=EBI-767597, EBI-767574;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus,
CC       nucleolus {ECO:0000269|PubMed:16823372}. Cytoplasm, cytoskeleton,
CC       microtubule organizing center, spindle pole body
CC       {ECO:0000269|PubMed:16823372}.
CC   -!- DISRUPTION PHENOTYPE: Cells have a nucleus that is cleaved by the
CC       septum or the septum divides the cell into a nucleate and anucleate
CC       compartment. {ECO:0000269|PubMed:9180692}.
CC   -!- SIMILARITY: Belongs to the HUS1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y09438; CAA70588.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAB11254.1; -; Genomic_DNA.
DR   PIR; T43396; T43396.
DR   RefSeq; NP_594739.1; NM_001020167.2.
DR   AlphaFoldDB; P78955; -.
DR   SMR; P78955; -.
DR   BioGRID; 278240; 187.
DR   IntAct; P78955; 1.
DR   STRING; 4896.SPAC20G4.04c.1; -.
DR   iPTMnet; P78955; -.
DR   PaxDb; P78955; -.
DR   EnsemblFungi; SPAC20G4.04c.1; SPAC20G4.04c.1:pep; SPAC20G4.04c.
DR   GeneID; 2541746; -.
DR   KEGG; spo:SPAC20G4.04c; -.
DR   PomBase; SPAC20G4.04c; hus1.
DR   VEuPathDB; FungiDB:SPAC20G4.04c; -.
DR   eggNOG; KOG3999; Eukaryota.
DR   HOGENOM; CLU_035754_0_0_1; -.
DR   InParanoid; P78955; -.
DR   OMA; PWASEDG; -.
DR   PhylomeDB; P78955; -.
DR   Reactome; R-SPO-176187; Activation of ATR in response to replication stress.
DR   PRO; PR:P78955; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0030896; C:checkpoint clamp complex; IDA:PomBase.
DR   GO; GO:0140445; C:chromosome, telomeric repeat region; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0044732; C:mitotic spindle pole body; HDA:PomBase.
DR   GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0035861; C:site of double-strand break; IDA:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR   GO; GO:0044778; P:meiotic DNA integrity checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IMP:PomBase.
DR   GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IMP:PomBase.
DR   GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR   GO; GO:0000723; P:telomere maintenance; IMP:PomBase.
DR   InterPro; IPR016580; Cell_cycle_HUS1.
DR   InterPro; IPR007150; Hus1/Mec3.
DR   Pfam; PF04005; Hus1; 1.
DR   PIRSF; PIRSF011312; Cell_cycle_HUS1; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; Cytoskeleton; DNA damage; Mitosis;
KW   Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..287
FT                   /note="Checkpoint protein hus1"
FT                   /id="PRO_0000084096"
FT   MOD_RES         210
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   287 AA;  32714 MW;  283CE8F538DEFB78 CRC64;
     MRFKTRISNL YTLTRLVQAL DKIGRFCWLR LMPETVNFVI VPDFRMTQVW SVLEVETIFE
     DYVVQSNADN VINLEVPIDN FYKALRSAAN ASDSTVRLSK KNNQPLLSLS TTWSGRAFGS
     NIVTHNIPVR VLSQSYVSVI KEPTAPEPDC HIFLPQLNFL RHVVDKYKSL SDRIIMSANM
     SGELQLSVNI PSARVSTKWK GLENPELDPS QVEDISRHPS QTRAPEEFVH MRLDSKDLVN
     MLKISSVAKR VIACFCEGHA LVLYVYITDP EDEHTAVLTY YISTYVD