HUT1_YEAST
ID HUT1_YEAST Reviewed; 339 AA.
AC Q12520; D6W3C7;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=UDP-galactose transporter homolog 1;
DE AltName: Full=Multicopy suppressor of leflunomide-sensitivity protein 6;
GN Name=HUT1; Synonyms=MLF6; OrderedLocusNames=YPL244C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=11391470; DOI=10.1007/s002840010267;
RA Fujimura H.;
RT "The Saccharomyces cerevisiae MLF6/YPL244C gene, which encodes a possible
RT yeast homologue of mammalian UDP-galactose transporter, confers resistance
RT to the immunosuppressive drug, leflunomide.";
RL Curr. Microbiol. 43:93-95(2001).
RN [5]
RP FUNCTION.
RX PubMed=11284009; DOI=10.1002/yea.708.abs;
RA Kainuma M., Chiba Y., Takeuchi M., Jigami Y.;
RT "Overexpression of HUT1 gene stimulates in vivo galactosylation by
RT enhancing UDP-galactose transport activity in Saccharomyces cerevisiae.";
RL Yeast 18:533-541(2001).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11284010; DOI=10.1002/yea.707.abs;
RA Nakanishi H., Nakayama K., Yokota A., Tachikawa H., Takahashi N.,
RA Jigami Y.;
RT "Hut1 proteins identified in Saccharomyces cerevisiae and
RT Schizosaccharomyces pombe are functional homologues involved in the
RT protein-folding process at the endoplasmic reticulum.";
RL Yeast 18:543-554(2001).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: May be involved in specific transport of UDP-Gal from the
CC cytosol to the Golgi lumen. Involved in the maintenance of optimal
CC conditions for the folding of secretory pathway proteins in the
CC endoplasmic reticulum. Overexpression confers resistance to the
CC immunosuppressive drug, leflunomide. {ECO:0000269|PubMed:11284009,
CC ECO:0000269|PubMed:11284010, ECO:0000269|PubMed:11391470}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:11284010}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11284010}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z67751; CAA91600.1; -; Genomic_DNA.
DR EMBL; Z73600; CAA97965.1; -; Genomic_DNA.
DR EMBL; AY692836; AAT92855.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11193.1; -; Genomic_DNA.
DR PIR; S61020; S61020.
DR RefSeq; NP_015080.1; NM_001184058.1.
DR AlphaFoldDB; Q12520; -.
DR SMR; Q12520; -.
DR BioGRID; 35919; 93.
DR DIP; DIP-3958N; -.
DR IntAct; Q12520; 1.
DR MINT; Q12520; -.
DR STRING; 4932.YPL244C; -.
DR TCDB; 2.A.7.11.6; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q12520; -.
DR PRIDE; Q12520; -.
DR EnsemblFungi; YPL244C_mRNA; YPL244C; YPL244C.
DR GeneID; 855832; -.
DR KEGG; sce:YPL244C; -.
DR SGD; S000006165; HUT1.
DR VEuPathDB; FungiDB:YPL244C; -.
DR eggNOG; KOG1581; Eukaryota.
DR GeneTree; ENSGT00940000157900; -.
DR HOGENOM; CLU_036019_0_2_1; -.
DR InParanoid; Q12520; -.
DR OMA; CGAIGQV; -.
DR BioCyc; YEAST:G3O-34130-MON; -.
DR PRO; PR:Q12520; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12520; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; ISS:SGD.
DR GO; GO:0005460; F:UDP-glucose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IMP:SGD.
DR GO; GO:0015786; P:UDP-glucose transmembrane transport; IMP:SGD.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..339
FT /note="UDP-galactose transporter homolog 1"
FT /id="PRO_0000213414"
FT TOPO_DOM 1..4
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..106
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..136
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..174
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..245
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..307
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 165
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 339 AA; 38075 MW; 1094A9D705BD3563 CRC64;
MAGSTSSLVI CAIGIYATFL TWALVQEPLA TRTWPNSMGK FQFPNVISLI QASVAMMMGY
LYLNWKKVEY PPRKMIKDHW KQLMLISFTQ SSSGPLATTS LKHVDYLTYM LAKSCKMIPV
LLVHLLLYRT PIASQKKVVA LLVSLGVTIF TIGGNDGKKL KRSFNESGND NKLQGFGLLF
SSLFLDGLTN ATQDKLLKAN KAKEKGKQTL ITGAHLMFTL NLFVILWNIL YFIVIDCKQW
DNAVSVLTMD PQVWGYLMLY SFCGAMGQCF IFYTLEQFGS LVLIMITVTR KMVSMILSII
VFGKSVRFQQ WVGMFIVFGG ITWEALNKKK ANIPKAKSA
