ID   HUTH_BRADU              Reviewed;         519 AA.
AC   Q89GV3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Histidine ammonia-lyase {ECO:0000255|HAMAP-Rule:MF_00229};
DE            Short=Histidase {ECO:0000255|HAMAP-Rule:MF_00229};
DE            EC=4.3.1.3 {ECO:0000255|HAMAP-Rule:MF_00229};
GN   Name=hutH {ECO:0000255|HAMAP-Rule:MF_00229}; OrderedLocusNames=bll6242;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-histidine = NH4(+) + trans-urocanate; Xref=Rhea:RHEA:21232,
CC         ChEBI:CHEBI:17771, ChEBI:CHEBI:28938, ChEBI:CHEBI:57595; EC=4.3.1.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00229};
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO), which
CC       is formed autocatalytically by cyclization and dehydration of residues
CC       Ala-Ser-Gly. {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- SIMILARITY: Belongs to the PAL/histidase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00229}.
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DR   EMBL; BA000040; BAC51507.1; -; Genomic_DNA.
DR   RefSeq; NP_772882.1; NC_004463.1.
DR   RefSeq; WP_011088982.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89GV3; -.
DR   SMR; Q89GV3; -.
DR   STRING; 224911.27354520; -.
DR   EnsemblBacteria; BAC51507; BAC51507; BAC51507.
DR   GeneID; 64026003; -.
DR   KEGG; bja:bll6242; -.
DR   PATRIC; fig|224911.44.peg.6215; -.
DR   eggNOG; COG2986; Bacteria.
DR   HOGENOM; CLU_014801_4_0_5; -.
DR   InParanoid; Q89GV3; -.
DR   OMA; CAPQVAG; -.
DR   PhylomeDB; Q89GV3; -.
DR   UniPathway; UPA00379; UER00549.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016841; F:ammonia-lyase activity; IBA:GO_Central.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IBA:GO_Central.
DR   GO; GO:0006548; P:histidine catabolic process; IBA:GO_Central.
DR   GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR   CDD; cd00332; PAL-HAL; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00229; His_ammonia_lyase; 1.
DR   InterPro; IPR001106; Aromatic_Lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   InterPro; IPR022313; Phe/His_NH3-lyase_AS.
DR   PANTHER; PTHR10362; PTHR10362; 1.
DR   Pfam; PF00221; Lyase_aromatic; 1.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Histidine metabolism; Lyase; Reference proteome.
FT   CHAIN           1..519
FT                   /note="Histidine ammonia-lyase"
FT                   /id="PRO_0000160994"
FT   MOD_RES         147
FT                   /note="2,3-didehydroalanine (Ser)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
FT   CROSSLNK        146..148
FT                   /note="5-imidazolinone (Ala-Gly)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
SQ   SEQUENCE   519 AA;  53119 MW;  755A8C2A49C6A10B CRC64;
     MTEQDAAIVV KPGTVSLDDL ARVLAGQPVV LDPSFWPRVE AAAAIVAKAA QADTPVYGIN
     TGFGKLASKR IPPDQTALLQ RNLIVSHCCG VGPATPEPIV RLMMALKIIS LGRGASGVRR
     EVIEQLQGML ARRVCPLVPQ QGSVGASGDL APLAHMTAVM IGEGQAIVDG KTVSGGEALA
     AAGLAPLTLG PKEGLALING TQFSTAYAIS GVLRGFHLAR AALVTGALSV DAAMASTAPF
     RPEIQALRGH AGQIAAAATL TALLDGSDIR LSHLEGDERV QDPYCLRCQP QVAGAALDLI
     TQTARALIVE ANAVTDNPLV LVETGEIVSG GNFHAEPVAF AADTIALALS EIGAISERRI
     ATLVDPALNF GLPPFLTPDP GVNSGFMIAE VTAAALYAEN KQRAAACSID STPTSANQED
     HVSMAAHAAR RLADMADNLA AILGIELLVA AQGITLRAPH ATSAPLVAVI AALREQVPAL
     GADRYMAGDL AKAAALVEAD ALPAAAIGVL PSDPFPRLA