ID   HUTH_DEIRA              Reviewed;         524 AA.
AC   Q9RZ06;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Histidine ammonia-lyase;
DE            Short=Histidase;
DE            EC=4.3.1.3;
GN   Name=hutH; OrderedLocusNames=DR_A0147;
OS   Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG
OS   4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC   Deinococcus.
OX   NCBI_TaxID=243230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA   White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA   Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA   Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA   Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA   Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA   Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA   Fraser C.M.;
RT   "Genome sequence of the radioresistant bacterium Deinococcus radiodurans
RT   R1.";
RL   Science 286:1571-1577(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-histidine = NH4(+) + trans-urocanate; Xref=Rhea:RHEA:21232,
CC         ChEBI:CHEBI:17771, ChEBI:CHEBI:28938, ChEBI:CHEBI:57595; EC=4.3.1.3;
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO), which
CC       is formed autocatalytically by cyclization and dehydration of residues
CC       Ala-Ser-Gly. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PAL/histidase family. {ECO:0000305}.
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DR   EMBL; AE001825; AAF12216.1; -; Genomic_DNA.
DR   PIR; F75610; F75610.
DR   RefSeq; NP_285471.1; NC_001264.1.
DR   RefSeq; WP_010889407.1; NZ_CP015082.1.
DR   AlphaFoldDB; Q9RZ06; -.
DR   SMR; Q9RZ06; -.
DR   STRING; 243230.DR_A0147; -.
DR   EnsemblBacteria; AAF12216; AAF12216; DR_A0147.
DR   KEGG; dra:DR_A0147; -.
DR   PATRIC; fig|243230.17.peg.3034; -.
DR   eggNOG; COG2986; Bacteria.
DR   HOGENOM; CLU_014801_4_0_0; -.
DR   InParanoid; Q9RZ06; -.
DR   OMA; CAPQVAG; -.
DR   OrthoDB; 715502at2; -.
DR   UniPathway; UPA00379; UER00549.
DR   Proteomes; UP000002524; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016841; F:ammonia-lyase activity; IBA:GO_Central.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IBA:GO_Central.
DR   GO; GO:0006548; P:histidine catabolic process; IBA:GO_Central.
DR   GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR   CDD; cd00332; PAL-HAL; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00229; His_ammonia_lyase; 1.
DR   InterPro; IPR001106; Aromatic_Lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   InterPro; IPR022313; Phe/His_NH3-lyase_AS.
DR   PANTHER; PTHR10362; PTHR10362; 1.
DR   Pfam; PF00221; Lyase_aromatic; 1.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Histidine metabolism; Lyase; Reference proteome.
FT   CHAIN           1..524
FT                   /note="Histidine ammonia-lyase"
FT                   /id="PRO_0000161002"
FT   REGION          500..524
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         140
FT                   /note="2,3-didehydroalanine (Ser)"
FT                   /evidence="ECO:0000250"
FT   CROSSLNK        139..141
FT                   /note="5-imidazolinone (Ala-Gly)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   524 AA;  55536 MW;  D7E7E32F139C6661 CRC64;
     MILDRDLNLE QFISVVRHGE QVELSAAARE RIARARTVIE QIVEGDTPIY GVNTGFGKFE
     NVQIDRSQLA QLQHNLIVSH AIGMGEPLPA EVVRGMLLLR AQSLSLGHSG VRVEVVELLL
     ALLNADALPV VPSQGSVGAS GDLAPLAHLA LGLIGLGDIE YQGQVRPAAD VLAELGLSPV
     QLQAKEGLAL INGTQLMGSL LALALHDAQV LLGTANLAAA MTVEARYGSH RPFQPDVVGL
     RPHPGALAVA AELREFLAGS EIAPSHLTGD GKVQDAYSLR AVPQVHGATW DALAQAERVL
     AVEFASVTDN PLIFPETGEV VSGGNFHGQP LAVTIDALKV AVAELGSISE RRTEQLLNPA
     LSGLPAFLTP NGGLNSGFMI AQYTSAALVS ENKVLSHPAS VDSIPTSANQ EDHVSMGAHA
     ARQLRQIVAN VQTVLSIELL CAAQGLDFQQ LRAGRGVQAA YEYVRTFVPT LTEDRYFRPD
     LLRLRGELVS GELLRVAQAA DTQAPAPAKL PDSGDEDRDT TSRH