HUTH_HALSA
ID HUTH_HALSA Reviewed; 525 AA.
AC Q9HQD5;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Probable histidine ammonia-lyase {ECO:0000255|HAMAP-Rule:MF_00229};
DE Short=Histidase {ECO:0000255|HAMAP-Rule:MF_00229};
DE EC=4.3.1.3 {ECO:0000255|HAMAP-Rule:MF_00229};
GN Name=hutH {ECO:0000255|HAMAP-Rule:MF_00229}; OrderedLocusNames=VNG_1212G;
OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS (Halobacterium halobium).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium.
OX NCBI_TaxID=64091;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX PubMed=11016950; DOI=10.1073/pnas.190337797;
RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA DasSarma S.;
RT "Genome sequence of Halobacterium species NRC-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidine = NH4(+) + trans-urocanate; Xref=Rhea:RHEA:21232,
CC ChEBI:CHEBI:17771, ChEBI:CHEBI:28938, ChEBI:CHEBI:57595; EC=4.3.1.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00229};
CC -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC {ECO:0000255|HAMAP-Rule:MF_00229}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00229}.
CC -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO), which
CC is formed autocatalytically by cyclization and dehydration of residues
CC Ala-Ser-Gly. {ECO:0000255|HAMAP-Rule:MF_00229}.
CC -!- SIMILARITY: Belongs to the PAL/histidase family. {ECO:0000255|HAMAP-
CC Rule:MF_00229}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG19580.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE004437; AAG19580.1; ALT_INIT; Genomic_DNA.
DR PIR; H84276; H84276.
DR RefSeq; WP_012289292.1; NC_002607.1.
DR AlphaFoldDB; Q9HQD5; -.
DR SMR; Q9HQD5; -.
DR STRING; 64091.VNG_1212G; -.
DR PaxDb; Q9HQD5; -.
DR EnsemblBacteria; AAG19580; AAG19580; VNG_1212G.
DR GeneID; 5953911; -.
DR KEGG; hal:VNG_1212G; -.
DR PATRIC; fig|64091.14.peg.929; -.
DR HOGENOM; CLU_014801_4_1_2; -.
DR InParanoid; Q9HQD5; -.
DR OrthoDB; 30804at2157; -.
DR PhylomeDB; Q9HQD5; -.
DR UniPathway; UPA00379; UER00549.
DR Proteomes; UP000000554; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016841; F:ammonia-lyase activity; IBA:GO_Central.
DR GO; GO:0004397; F:histidine ammonia-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR CDD; cd00332; PAL-HAL; 1.
DR Gene3D; 1.10.275.10; -; 1.
DR HAMAP; MF_00229; His_ammonia_lyase; 1.
DR InterPro; IPR001106; Aromatic_Lyase.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR005921; HutH.
DR InterPro; IPR008948; L-Aspartase-like.
DR InterPro; IPR022313; Phe/His_NH3-lyase_AS.
DR PANTHER; PTHR10362; PTHR10362; 1.
DR Pfam; PF00221; Lyase_aromatic; 1.
DR SUPFAM; SSF48557; SSF48557; 1.
DR TIGRFAMs; TIGR01225; hutH; 1.
DR PROSITE; PS00488; PAL_HISTIDASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Histidine metabolism; Lyase; Reference proteome.
FT CHAIN 1..525
FT /note="Probable histidine ammonia-lyase"
FT /id="PRO_0000161055"
FT MOD_RES 146
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
FT CROSSLNK 145..147
FT /note="5-imidazolinone (Ala-Gly)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
SQ SEQUENCE 525 AA; 54073 MW; D020924892D9D520 CRC64;
MTTEGVVIDG ESLTPAAVDA VARHDAPVSI AAGAREAVRE SRARIADVLG GDEAVYGVNT
GFGSMSDTRI DAADREALQA NLVRSHAAGA GSELDTAAVR ALLVTRLNAL AKGYSGIRER
VLDVLVGLLN EGVHPVVPSR GSLGASGDLA PLAHMSRVLI GEGQADVAGE RMPAAEALAA
ADLEPVTLQA KEGLALINGT QLTTGVAALA LVDAERVLRS ADTAGALTTE VTMSTTASCA
PAIHEVRPHD GQAVSARHIR NLTAGSEVLD HHRDCDRVQD AYSIRCLPQV HGAVRDALDH
LRAAVATELN SATDNPLVFA GDRVGPRASG TDRAAVVSGG NFHGEVLALR LGYAASALAE
LAAISERRTD RLLNPETQEP HLEPFLAPDS GLHSGLMIPQ YTAASLVNDL RSLGQPTLDN
ASVSGAQEDH VSMSAGAAYN FREAVEKAAT VVGVELLCGA QGREFLDPDL ALGAGTAAAY
DLVREVSEPV AGDRALADDM AAVGDLVRAG LVEDAVARAL DAPLA