ID   HUTH_PSEAE              Reviewed;         509 AA.
AC   Q9HU85;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Histidine ammonia-lyase {ECO:0000255|HAMAP-Rule:MF_00229};
DE            Short=Histidase {ECO:0000255|HAMAP-Rule:MF_00229};
DE            EC=4.3.1.3 {ECO:0000255|HAMAP-Rule:MF_00229};
GN   Name=hutH {ECO:0000255|HAMAP-Rule:MF_00229}; OrderedLocusNames=PA5098;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-histidine = NH4(+) + trans-urocanate; Xref=Rhea:RHEA:21232,
CC         ChEBI:CHEBI:17771, ChEBI:CHEBI:28938, ChEBI:CHEBI:57595; EC=4.3.1.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00229};
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO), which
CC       is formed autocatalytically by cyclization and dehydration of residues
CC       Ala-Ser-Gly. {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- SIMILARITY: Belongs to the PAL/histidase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00229}.
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DR   EMBL; AE004091; AAG08483.1; -; Genomic_DNA.
DR   PIR; G83009; G83009.
DR   RefSeq; NP_253785.1; NC_002516.2.
DR   RefSeq; WP_003114461.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HU85; -.
DR   SMR; Q9HU85; -.
DR   STRING; 287.DR97_2452; -.
DR   PaxDb; Q9HU85; -.
DR   PRIDE; Q9HU85; -.
DR   EnsemblBacteria; AAG08483; AAG08483; PA5098.
DR   GeneID; 878368; -.
DR   KEGG; pae:PA5098; -.
DR   PATRIC; fig|208964.12.peg.5343; -.
DR   PseudoCAP; PA5098; -.
DR   HOGENOM; CLU_014801_4_0_6; -.
DR   InParanoid; Q9HU85; -.
DR   OMA; CAPQVAG; -.
DR   PhylomeDB; Q9HU85; -.
DR   BioCyc; PAER208964:G1FZ6-5213-MON; -.
DR   UniPathway; UPA00379; UER00549.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016841; F:ammonia-lyase activity; IBA:GO_Central.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IMP:PseudoCAP.
DR   GO; GO:0006548; P:histidine catabolic process; IMP:PseudoCAP.
DR   GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR   CDD; cd00332; PAL-HAL; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00229; His_ammonia_lyase; 1.
DR   InterPro; IPR001106; Aromatic_Lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   InterPro; IPR022313; Phe/His_NH3-lyase_AS.
DR   PANTHER; PTHR10362; PTHR10362; 1.
DR   Pfam; PF00221; Lyase_aromatic; 1.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Histidine metabolism; Lyase; Reference proteome.
FT   CHAIN           1..509
FT                   /note="Histidine ammonia-lyase"
FT                   /id="PRO_0000161015"
FT   MOD_RES         143
FT                   /note="2,3-didehydroalanine (Ser)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
FT   CROSSLNK        142..144
FT                   /note="5-imidazolinone (Ala-Gly)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
SQ   SEQUENCE   509 AA;  53780 MW;  094E0644B45B64DE CRC64;
     MSLHLKPGQL TLADLRQAYL APVRLSLDPS ADAPIAASVA CVENIIAEGR TAYGINTGFG
     LLASTRISPA DLEKLQRSIV LSHAAGVGEA LDDAMVRLVM LLKVNSLARG FSGIRRKVID
     ALIALINAEV YPHIPLKGSV GASGDLAPLA HMSLVLIGES RARHRGEWLP AAEALAVAGL
     EPLTLAAKEG LALLNGTQVS TAYALRGLFE AEDLFAAATV CGGLSVEAML GSRAPFDARI
     HAARGQRGQI DVAAAYRDLL TASSEVARSH EKCDKVQDPY SLRCQPQVMG ACLTQMRQAA
     EVLEIEANAV SDNPLVFAAE GDVISGGNFH AEPVAMAADN LALALAEIGS LSERRISLMM
     DMHMSQLPPF LVANGGVNSG FMIAQVTAAA LASDNKALAH PASVDSLPTS ANQEDHVSMA
     PNAGKRLWAM AENVRGILAV EWLGACQGLD FREGLKSSPK LEQARRLLRD KVPYYQEDRF
     FAPDIEAASQ LLASGCLNAL LPARLLPSL