ID   APAH_BURMA              Reviewed;         282 AA.
AC   Q9AEV8; Q62I92;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Bis(5'-nucleosyl)-tetraphosphatase, symmetrical {ECO:0000255|HAMAP-Rule:MF_00199};
DE            EC=3.6.1.41 {ECO:0000255|HAMAP-Rule:MF_00199};
DE   AltName: Full=Ap4A hydrolase {ECO:0000255|HAMAP-Rule:MF_00199};
DE   AltName: Full=Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase {ECO:0000255|HAMAP-Rule:MF_00199};
DE   AltName: Full=Diadenosine tetraphosphatase {ECO:0000255|HAMAP-Rule:MF_00199};
GN   Name=apaH {ECO:0000255|HAMAP-Rule:MF_00199}; OrderedLocusNames=BMA1991;
OS   Burkholderia mallei (strain ATCC 23344).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=243160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11790757; DOI=10.1128/jb.184.3.849-852.2002;
RA   Burtnick M.N., Brett P.J., Woods D.E.;
RT   "Molecular and physical characterization of Burkholderia mallei O
RT   antigens.";
RL   J. Bacteriol. 184:849-852(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23344;
RX   PubMed=15377793; DOI=10.1073/pnas.0403306101;
RA   Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E.,
RA   Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C.,
RA   Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S.,
RA   Gwinn M.L., Haft D.H., Khouri H.M., Kolonay J.F., Madupu R., Mohammoud Y.,
RA   Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C.,
RA   Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M.;
RT   "Structural flexibility in the Burkholderia mallei genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004).
CC   -!- FUNCTION: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield
CC       ADP. {ECO:0000255|HAMAP-Rule:MF_00199}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2
CC         H(+); Xref=Rhea:RHEA:24252, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58141, ChEBI:CHEBI:456216; EC=3.6.1.41;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00199};
CC   -!- SIMILARITY: Belongs to the Ap4A hydrolase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00199}.
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DR   EMBL; AY028370; AAK27390.1; -; Genomic_DNA.
DR   EMBL; CP000010; AAU49991.1; -; Genomic_DNA.
DR   RefSeq; WP_004186146.1; NC_006348.1.
DR   RefSeq; YP_103578.1; NC_006348.1.
DR   AlphaFoldDB; Q9AEV8; -.
DR   SMR; Q9AEV8; -.
DR   STRING; 243160.BMA1991; -.
DR   EnsemblBacteria; AAU49991; AAU49991; BMA1991.
DR   GeneID; 56594993; -.
DR   KEGG; bma:BMA1991; -.
DR   PATRIC; fig|243160.12.peg.2058; -.
DR   eggNOG; COG0639; Bacteria.
DR   HOGENOM; CLU_056184_1_0_4; -.
DR   OMA; INAFTRM; -.
DR   Proteomes; UP000006693; Chromosome 1.
DR   GO; GO:0008803; F:bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity; IEA:UniProtKB-UniRule.
DR   CDD; cd07422; MPP_ApaH; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   HAMAP; MF_00199; ApaH; 1.
DR   InterPro; IPR004617; ApaH.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF00149; Metallophos; 1.
DR   PIRSF; PIRSF000903; B5n-ttraPtase_sm; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
DR   TIGRFAMs; TIGR00668; apaH; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..282
FT                   /note="Bis(5'-nucleosyl)-tetraphosphatase, symmetrical"
FT                   /id="PRO_0000197984"
FT   CONFLICT        90
FT                   /note="T -> S (in Ref. 1; AAK27390)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        96
FT                   /note="D -> Y (in Ref. 1; AAK27390)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        280
FT                   /note="A -> P (in Ref. 1; AAK27390)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   282 AA;  30572 MW;  665D4AA4D13EDF9D CRC64;
     MTNFSSSPPI AFGDLQGCHA AYRQLFDTLA PAADTPLWFA GDLVNRGPAS LATLREIAAL
     GERAIAVLGN HDLHLLAVAA GIRTLKPGDT IGEILDAPDA DDLIEWVRHR PFAHFERGML
     MVHAGLLPQW DAALALELAD ELQRALRASN WRDTLRSLYG NDPNCWSPDL KHADRLRVAF
     NAFTRIRFCT PEGAMEFRAN GGPAAAPAGY LPWFDAPGRK TADVTVVFGH WAALGLMLRE
     NLVALDSGCV WGNRLSAVRL ADDPAARVVT QVACERCGAA DE