ID   HUTH_THEVO              Reviewed;         495 AA.
AC   Q978N8;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Probable histidine ammonia-lyase {ECO:0000255|HAMAP-Rule:MF_00229};
DE            Short=Histidase {ECO:0000255|HAMAP-Rule:MF_00229};
DE            EC=4.3.1.3 {ECO:0000255|HAMAP-Rule:MF_00229};
GN   Name=hutH {ECO:0000255|HAMAP-Rule:MF_00229}; OrderedLocusNames=TV1377;
GN   ORFNames=TVG1425265;
OS   Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC
OS   15438 / GSS1).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Thermoplasmataceae; Thermoplasma.
OX   NCBI_TaxID=273116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1;
RX   PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA   Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y.,
RA   Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T.,
RA   Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT   "Archaeal adaptation to higher temperatures revealed by genomic sequence of
RT   Thermoplasma volcanium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-histidine = NH4(+) + trans-urocanate; Xref=Rhea:RHEA:21232,
CC         ChEBI:CHEBI:17771, ChEBI:CHEBI:28938, ChEBI:CHEBI:57595; EC=4.3.1.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00229};
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO), which
CC       is formed autocatalytically by cyclization and dehydration of residues
CC       Ala-Ser-Gly. {ECO:0000255|HAMAP-Rule:MF_00229}.
CC   -!- SIMILARITY: Belongs to the PAL/histidase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00229}.
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DR   EMBL; BA000011; BAB60519.1; -; Genomic_DNA.
DR   RefSeq; WP_010917611.1; NC_002689.2.
DR   AlphaFoldDB; Q978N8; -.
DR   SMR; Q978N8; -.
DR   STRING; 273116.14325616; -.
DR   EnsemblBacteria; BAB60519; BAB60519; BAB60519.
DR   GeneID; 1442069; -.
DR   KEGG; tvo:TVG1425265; -.
DR   eggNOG; arCOG04671; Archaea.
DR   HOGENOM; CLU_014801_4_0_2; -.
DR   OMA; CAPQVAG; -.
DR   OrthoDB; 30804at2157; -.
DR   PhylomeDB; Q978N8; -.
DR   UniPathway; UPA00379; UER00549.
DR   Proteomes; UP000001017; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR   CDD; cd00332; PAL-HAL; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00229; His_ammonia_lyase; 1.
DR   InterPro; IPR001106; Aromatic_Lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   InterPro; IPR022313; Phe/His_NH3-lyase_AS.
DR   PANTHER; PTHR10362; PTHR10362; 1.
DR   Pfam; PF00221; Lyase_aromatic; 1.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Histidine metabolism; Lyase.
FT   CHAIN           1..495
FT                   /note="Probable histidine ammonia-lyase"
FT                   /id="PRO_0000161057"
FT   MOD_RES         142
FT                   /note="2,3-didehydroalanine (Ser)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
FT   CROSSLNK        141..143
FT                   /note="5-imidazolinone (Ala-Gly)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00229"
SQ   SEQUENCE   495 AA;  53956 MW;  AB7AB91B06345EEB CRC64;
     MIEIDGDNLT LEDVYSVSVL HEPVELSTKA RNKVAEIHRK FLDLISSGQT IYGVNTGFGG
     LLNIKISREE EIELQRNLIR SHSAGVGKYL PTDVVRSIMV IRANTLAKGY SAVSTELIDA
     LLAMINKDVV PAVPEFGSVG ASGDLAPLAH IGLAMMGEGQ AFLNGELMRS DIALSKVGLK
     PYEFKEKEGV ALINGTSMMA GIMALVTSRA YRTIENAVRS SLLSFEALRG TSKAFSDWIV
     SARPHLGQIA IAEKMRKYLA GSKNVEKSDK EKVQDAYTLR CIPQVYGAVL DTLEYVSSVL
     VTEINSATDN PLFNGKEVVS GGNFHGEPIA LAADFLSIAL TDMGNMIERR IARLVDTNLS
     GLPPFLVKNS GLNSGYMIPQ YTAAALCNRN KVLSYPSSAD SIPTSANQED HVSMGSTSTL
     KLLEIEENLE YIVAIEFLLG AQALEFSQDP ISPVTKAIYT KIREYVKPLD KDRPSYLDIE
     KIREIMNKNE LINAA