HUTU_CAEEL
ID HUTU_CAEEL Reviewed; 670 AA.
AC Q9NAE2;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable urocanate hydratase;
DE Short=Urocanase;
DE EC=4.2.1.49;
DE AltName: Full=Imidazolonepropionate hydrolase;
GN ORFNames=Y51H4A.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-imidazolone-5-propanoate = H2O + trans-urocanate;
CC Xref=Rhea:RHEA:13101, ChEBI:CHEBI:15377, ChEBI:CHEBI:17771,
CC ChEBI:CHEBI:77893; EC=4.2.1.49;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3.
CC -!- SIMILARITY: Belongs to the urocanase family. {ECO:0000305}.
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DR EMBL; AL132952; CAB61139.2; -; Genomic_DNA.
DR RefSeq; NP_502964.2; NM_070563.4.
DR AlphaFoldDB; Q9NAE2; -.
DR SMR; Q9NAE2; -.
DR BioGRID; 43538; 13.
DR STRING; 6239.Y51H4A.7.1; -.
DR EPD; Q9NAE2; -.
DR PaxDb; Q9NAE2; -.
DR PeptideAtlas; Q9NAE2; -.
DR EnsemblMetazoa; Y51H4A.7.1; Y51H4A.7.1; WBGene00013103.
DR EnsemblMetazoa; Y51H4A.7.2; Y51H4A.7.2; WBGene00013103.
DR UCSC; Y51H4A.7.1; c. elegans.
DR WormBase; Y51H4A.7; CE35671; WBGene00013103; -.
DR eggNOG; ENOG502QR75; Eukaryota.
DR GeneTree; ENSGT00390000015136; -.
DR HOGENOM; CLU_018868_3_0_1; -.
DR InParanoid; Q9NAE2; -.
DR OMA; LVGDWAN; -.
DR OrthoDB; 536177at2759; -.
DR PhylomeDB; Q9NAE2; -.
DR Reactome; R-CEL-70921; Histidine catabolism.
DR UniPathway; UPA00379; UER00550.
DR PRO; PR:Q9NAE2; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00013103; Expressed in larva and 3 other tissues.
DR GO; GO:0016153; F:urocanate hydratase activity; IBA:GO_Central.
DR GO; GO:0006548; P:histidine catabolic process; IBA:GO_Central.
DR GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.10730; -; 1.
DR HAMAP; MF_00577; HutU; 1.
DR InterPro; IPR023637; Urocanase.
DR InterPro; IPR035401; Urocanase_C.
DR InterPro; IPR038364; Urocanase_central_sf.
DR InterPro; IPR023636; Urocanase_CS.
DR InterPro; IPR035400; Urocanase_N.
DR InterPro; IPR035085; Urocanase_Rossmann-like.
DR InterPro; IPR036190; Urocanase_sf.
DR PANTHER; PTHR12216; PTHR12216; 1.
DR Pfam; PF01175; Urocanase; 1.
DR Pfam; PF17392; Urocanase_C; 1.
DR Pfam; PF17391; Urocanase_N; 1.
DR PIRSF; PIRSF001423; Urocanate_hydrat; 1.
DR SUPFAM; SSF111326; SSF111326; 1.
DR PROSITE; PS01233; UROCANASE; 1.
PE 3: Inferred from homology;
KW Histidine metabolism; Lyase; NAD; Reference proteome.
FT CHAIN 1..670
FT /note="Probable urocanate hydratase"
FT /id="PRO_0000207376"
FT BINDING 126..127
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 204
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 250..252
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 270
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 316..317
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 338..342
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 349..350
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 398
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 590
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
SQ SEQUENCE 670 AA; 74407 MW; C0BFBFE972DBAFDC CRC64;
MNIPSLFVDP FSPLLEHPTE QRAKNVAHAP KRPCNLTQTE KMLAVRNALR YIPKEHHVLL
ATEFAEELNT YGHIYGYRFM PNFDLFAPPV SEIGAHCEQA SAIILMILNN LDKRVAQFPQ
ELVTYGGNGQ VFSNWIQFRL VLRYLYTMTD HQTLVLYSGH PLGLFPSTPD SPRMTVTNGM
MIPSYSTKEL YDKYFALGVT QYGQMTAGSF CYIGPQGIVH GTTITVLNAG RRMGLDSLAG
KVFVTAGLGG MSGAQPKAAK IAGCIGVIAE ISDTALLKRH QQGWLDVYSK DLEEIVNWIK
EYREKKEAIS IGYLGNVVDL WERLAEEPEC LVELGSDQTS LHNPFLGGFY PAGLTFEQSN
QMMTSDPVKF KKLVQNSLIR QIAAIDKIAA KGMYFWDYGN AFLLECQRAG ANLLREDAQD
DKSFRYPSYM QDIMGDIFSM GFGPFRWVCT SGKPEDLRLT DQTACKIIDE LKDTDVPEYV
KQQYLDNKKW IEEAEKNKLV VGSQARILYS DRAGRVALAS AFNELVKSGK VSAAIVISRD
HHDVSGTDSP FRETSNVYDG SAFTADMAVQ NCIGDSFRGA TWVALHNGGG VGWGDVINGG
FGIVLDGSSD AARRAEGMLN WDVPNGVTRR SWSGNAKAQE AIQRAEKQVD GLRVTLPVEA
DEELLKKLKF
