HUTU_DICDI
ID HUTU_DICDI Reviewed; 672 AA.
AC Q86AX3; B0M0P6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Probable urocanate hydratase;
DE Short=Urocanase;
DE EC=4.2.1.49;
DE AltName: Full=Imidazolonepropionate hydrolase;
GN Name=uroc1; ORFNames=DDB_G0277727;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-imidazolone-5-propanoate = H2O + trans-urocanate;
CC Xref=Rhea:RHEA:13101, ChEBI:CHEBI:15377, ChEBI:CHEBI:17771,
CC ChEBI:CHEBI:77893; EC=4.2.1.49;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3.
CC -!- SIMILARITY: Belongs to the urocanase family. {ECO:0000305}.
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DR EMBL; AAFI02000022; EAL68537.2; -; Genomic_DNA.
DR RefSeq; XP_642464.2; XM_637372.3.
DR AlphaFoldDB; Q86AX3; -.
DR SMR; Q86AX3; -.
DR STRING; 44689.DDB0234152; -.
DR PaxDb; Q86AX3; -.
DR GeneID; 8621175; -.
DR KEGG; ddi:DDB_G0277727; -.
DR dictyBase; DDB_G0277727; uroc1.
DR eggNOG; ENOG502QR75; Eukaryota.
DR HOGENOM; CLU_018868_3_0_1; -.
DR InParanoid; Q86AX3; -.
DR OMA; LVGDWAN; -.
DR PhylomeDB; Q86AX3; -.
DR Reactome; R-DDI-70921; Histidine catabolism.
DR UniPathway; UPA00379; UER00550.
DR PRO; PR:Q86AX3; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0016153; F:urocanate hydratase activity; IBA:GO_Central.
DR GO; GO:0006548; P:histidine catabolic process; IBA:GO_Central.
DR GO; GO:0019556; P:histidine catabolic process to glutamate and formamide; IEA:UniProtKB-UniPathway.
DR GO; GO:0019557; P:histidine catabolic process to glutamate and formate; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.10730; -; 1.
DR HAMAP; MF_00577; HutU; 1.
DR InterPro; IPR023637; Urocanase.
DR InterPro; IPR035401; Urocanase_C.
DR InterPro; IPR038364; Urocanase_central_sf.
DR InterPro; IPR035400; Urocanase_N.
DR InterPro; IPR035085; Urocanase_Rossmann-like.
DR InterPro; IPR036190; Urocanase_sf.
DR PANTHER; PTHR12216; PTHR12216; 1.
DR Pfam; PF01175; Urocanase; 1.
DR Pfam; PF17392; Urocanase_C; 1.
DR Pfam; PF17391; Urocanase_N; 1.
DR PIRSF; PIRSF001423; Urocanate_hydrat; 1.
DR SUPFAM; SSF111326; SSF111326; 1.
PE 3: Inferred from homology;
KW Histidine metabolism; Lyase; NAD; Reference proteome.
FT CHAIN 1..672
FT /note="Probable urocanate hydratase"
FT /id="PRO_0000328400"
FT BINDING 128..129
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 206
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 253..255
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 273
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 318..319
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 340..344
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 351..352
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 400
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
FT BINDING 592
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25503"
SQ SEQUENCE 672 AA; 74813 MW; 34B49D563A978EC1 CRC64;
MKDLLSSLSK GIPFEPLPEH PGLDSTVPHA PGRPINLTLN EKKLAIKNSL RYFPNSLHSI
LANEFLEELN KYGHIYMYRF RPTHYEMKAY PIEEYPAKSR QAASIMLMIM NNLDREVAQF
PNELVTYGGN GSVFQNWAQY HLTMKYLSEM TDQQTLTMYS GHPMGLFPSN ESSPRVIVTN
GMVIPNYSSH KDYERMYAQG VTQYGQMTAG SYCYIGPQGI VHGTTITLLN AGRKYLGVES
LIGKVFVSSG LGGMSGAQTK AAYICGAICI VAEVSPAAVK KRHAQNWVME VYSDLNELFA
RGSCSKEKKP LSIAYQGNVV TLWEKLVEEK DITVDLGSDQ TSLHNPFNGG YYPVQCSFEE
SNRLMVQEPT KFKEMVQETL RRHTTAINAL SARGMKFWDY GNSFLLEASR AKADIIKPGT
DGKEFIYPSY VQDIMGDIFS LGFGPFRWVC TSGNPKDLEI TDRIAKELIE KLRSQPGVPK
NVQQQFNDNH LWISQAGEHK LVVGSQARIL YSDCIGRSEL AVAFNKAVAD GTLSAPVVIS
RDHHDVSGTD APWRETSNIK DGSQFCADMA IHNVIGDSFR GATWVAIHNG GGTGFGEAIN
GGFGLYLCGS KLQEEKARMM LNWDVNNGIA RRSWSYNDNA ENTIKRAMEL DPSLKVTIPN
HTSDDLINSL NF
