ID   APAH_KLEAE              Reviewed;         259 AA.
AC   P27510;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Bis(5'-nucleosyl)-tetraphosphatase, symmetrical;
DE            EC=3.6.1.41;
DE   AltName: Full=Ap4A hydrolase;
DE   AltName: Full=Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase;
DE   AltName: Full=Diadenosine tetraphosphatase;
DE   Flags: Fragment;
GN   Name=apaH;
OS   Klebsiella aerogenes (Enterobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W70;
RX   PubMed=1551851; DOI=10.1128/jb.174.7.2344-2351.1992;
RA   Azakami H., Sugino H., Murooka Y.;
RT   "Cloning and nucleotide sequence of a negative regulator gene for
RT   Klebsiella aerogenes arylsulfatase synthesis and identification of the gene
RT   as folA.";
RL   J. Bacteriol. 174:2344-2351(1992).
CC   -!- FUNCTION: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield
CC       ADP. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2
CC         H(+); Xref=Rhea:RHEA:24252, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58141, ChEBI:CHEBI:456216; EC=3.6.1.41;
CC   -!- SIMILARITY: Belongs to the Ap4A hydrolase family. {ECO:0000305}.
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DR   EMBL; D10358; BAA01188.1; -; Genomic_DNA.
DR   AlphaFoldDB; P27510; -.
DR   SMR; P27510; -.
DR   STRING; 548.EAG7_03292; -.
DR   PRIDE; P27510; -.
DR   GO; GO:0008803; F:bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity; IEA:UniProtKB-EC.
DR   CDD; cd07422; MPP_ApaH; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   HAMAP; MF_00199; ApaH; 1.
DR   InterPro; IPR004617; ApaH.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF00149; Metallophos; 1.
DR   PIRSF; PIRSF000903; B5n-ttraPtase_sm; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
DR   TIGRFAMs; TIGR00668; apaH; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           <1..259
FT                   /note="Bis(5'-nucleosyl)-tetraphosphatase, symmetrical"
FT                   /id="PRO_0000197994"
FT   NON_TER         1
SQ   SEQUENCE   259 AA;  29115 MW;  8755312AAC7AF7CD CRC64;
     EFDPRRDTLW LTGDLVARGP GSLEVLRYVK SLGDTVRLVL GNHDLHLLAV FAGISRNKPK
     DRLKPLLEAP DADELLNWLR RQPLLQVDEE KKLVMAHAGI TPQWDLETAQ QCARDVEAVL
     SSDSYPFFLD AMYGDMPNHW SNELSGLARL RFISNAFTRM RYCFPNGQLD MYSKEAPEDA
     PAPLKPWFAI PGPVSNAYSI AFGHWASLEG RGTPEGIYAL DTGCCWGGEL TCLRWEDKQY
     FTQPSNRQKS LDEGEAVAS