HXA4_MOUSE
ID HXA4_MOUSE Reviewed; 285 AA.
AC P06798; Q61684; Q64388; Q8BPE6;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 4.
DT 25-MAY-2022, entry version 190.
DE RecName: Full=Homeobox protein Hox-A4;
DE AltName: Full=Homeobox protein Hox-1.4;
DE AltName: Full=Homeobox protein MH-3;
GN Name=Hoxa4; Synonyms=Hox-1.4, Hoxa-4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7909702;
RA Viviano C.M., Galliot B., Wolgemuth D.J.;
RT "Multiple levels of regulation exist for expression of the Hoxa-4 (Hox-1.4)
RT gene in the mouse testis.";
RL Cell. Mol. Biol. Res. 39:483-495(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7901228; DOI=10.1002/jcb.240520409;
RA Wu K., Wolgemuth D.J.;
RT "Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4)
RT gene binds to homeobox consensus binding sites in its promoter and
RT intron.";
RL J. Cell. Biochem. 52:449-462(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
RX PubMed=2576648; DOI=10.1242/dev.107.2.343;
RA Galliot B., Dolle P., Vigneron M., Featherstone M.S., Baron A., Duboule D.;
RT "The mouse Hox-1.4 gene: primary structure, evidence for promoter activity
RT and expression during development.";
RL Development 107:343-359(1989).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-199, AND NUCLEOTIDE SEQUENCE
RP [MRNA] OF 180-285.
RC STRAIN=ICR; TISSUE=Spinal cord, and Testis;
RX PubMed=1686835; DOI=10.3109/10425179109020787;
RA Rubin M.R., Nguyen-Huu M.C.;
RT "Murine embryonic spinal cord and adult testis Hox-1.4 cDNAs are identical
RT 3' to the homeo box.";
RL DNA Seq. 1:329-334(1991).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 180-285.
RX PubMed=2895600; DOI=10.1111/j.1749-6632.1987.tb25002.x;
RA Duggal R.N., Zakeri Z.F., Ponzetto C., Wolgemuth D.J.;
RT "Differential expression of the c-abl proto-oncogene and the homeo box-
RT containing gene Hox 1.4 during mouse spermatogenesis.";
RL Ann. N. Y. Acad. Sci. 513:112-127(1987).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 180-273.
RX PubMed=3726554; DOI=10.1126/science.3726554;
RA Rubin M.R., Toth L.E., Patel M.D., D'Eustachio P., Nguyen-Huu M.C.;
RT "A mouse homeo box gene is expressed in spermatocytes and embryos.";
RL Science 233:663-667(1986).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 198-271.
RX PubMed=2426103; DOI=10.1002/j.1460-2075.1986.tb04351.x;
RA Wolgemuth D.J., Engelmyer E., Duggal R.N., Gizang-Ginsberg E., Mutter G.L.,
RA Ponzetto C., Viviano C., Zakeri Z.F.;
RT "Isolation of a mouse cDNA coding for a developmentally regulated, testis-
RT specific transcript containing homeo box homology.";
RL EMBO J. 5:1229-1235(1986).
RN [9]
RP RESULTS OF OVEREXPRESSION.
RX PubMed=2563568; DOI=10.1038/337464a0;
RA Wolgemuth D.J., Behringer R.R., Mostoller M.P., Brinster R.L.,
RA Palmiter R.D.;
RT "Transgenic mice overexpressing the mouse homoeobox-containing gene Hox-1.4
RT exhibit abnormal gut development.";
RL Nature 337:464-467(1989).
CC -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC developmental regulatory system that provides cells with specific
CC positional identities on the anterior-posterior axis. Binds to sites in
CC the 5'-flanking sequence of its coding region with various affinities.
CC The consensus sequences of the high and low affinity binding sites are
CC 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: During development of the prepuberal testis high
CC levels of HOXA4 transcripts were found at days 17, 24 and 30. The first
CC day of HOXA4 expression was day 14. The activation of the HOXA4 gene in
CC male germ cells seems to occur at the pachytene stage of meiotic
CC prophase and its level of expression is stage-specific during
CC embryogenesis.
CC -!- DISEASE: Note=Overexpression results in abnormal gut development
CC (megacolon).
CC -!- SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily.
CC {ECO:0000305}.
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DR EMBL; S70444; AAB30705.2; -; Genomic_DNA.
DR EMBL; S67058; AAB28662.2; -; Genomic_DNA.
DR EMBL; AK076098; BAC36181.1; -; mRNA.
DR EMBL; X66861; CAA47330.1; -; Genomic_DNA.
DR EMBL; X13538; CAA31889.1; -; mRNA.
DR EMBL; X17346; CAA35228.1; -; Genomic_DNA.
DR EMBL; M27432; AAA16440.1; -; mRNA.
DR EMBL; M13813; AAA37831.1; -; mRNA.
DR CCDS; CCDS20142.1; -.
DR PIR; A43556; A43556.
DR RefSeq; NP_032291.1; NM_008265.3.
DR AlphaFoldDB; P06798; -.
DR SMR; P06798; -.
DR BioGRID; 200369; 8.
DR IntAct; P06798; 1.
DR STRING; 10090.ENSMUSP00000098943; -.
DR iPTMnet; P06798; -.
DR PhosphoSitePlus; P06798; -.
DR MaxQB; P06798; -.
DR PaxDb; P06798; -.
DR PeptideAtlas; P06798; -.
DR PRIDE; P06798; -.
DR ProteomicsDB; 273327; -.
DR DNASU; 15401; -.
DR GeneID; 15401; -.
DR KEGG; mmu:15401; -.
DR UCSC; uc009byf.1; mouse.
DR CTD; 3201; -.
DR MGI; MGI:96176; Hoxa4.
DR eggNOG; KOG0489; Eukaryota.
DR InParanoid; P06798; -.
DR OrthoDB; 1154159at2759; -.
DR PhylomeDB; P06798; -.
DR TreeFam; TF352857; -.
DR BioGRID-ORCS; 15401; 6 hits in 74 CRISPR screens.
DR PRO; PR:P06798; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P06798; protein.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; TAS:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0048704; P:embryonic skeletal system morphogenesis; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017995; Homeobox_antennapedia.
DR InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00025; ANTENNAPEDIA.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00032; ANTENNAPEDIA; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..285
FT /note="Homeobox protein Hox-A4"
FT /id="PRO_0000200049"
FT DNA_BIND 180..239
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 19..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 159..164
FT /note="Antp-type hexapeptide"
FT COMPBIAS 45..61
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..123
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 97
FT /note="G -> A (in Ref. 3; BAC36181 and 4; CAA47330)"
FT /evidence="ECO:0000305"
FT CONFLICT 117..120
FT /note="VPLG -> APPR (in Ref. 3; BAC36181)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="K -> E (in Ref. 5; CAA35228 and 7; AAA37831)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="Missing (in Ref. 7; AAA37831)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="H -> P (in Ref. 1, 2 and 3; BAC36181)"
FT /evidence="ECO:0000305"
FT CONFLICT 272..273
FT /note="HP -> PE (in Ref. 7)"
FT /evidence="ECO:0000305"
FT CONFLICT 279..285
FT /note="TPIPSSI -> HPFPPPYNLETWISFSHLCALLISAPVLICSSLSKPETPKQ
FT NPTCWKP (in Ref. 5; CAA31889)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 285 AA; 30468 MW; 475D48D6C6302A26 CRC64;
MTMSSFLINS NYIEPKFPPF EEFAPHGGPG GGDGAVGGGP GYPRPQSAPH LPAPNPHAAR
QPPAYYAPRA REPSYPGGLY PAPAAACPYA CRGASPGRPE QSPAPGAHPS PAPQPPVPLG
HCAPGPTTPA VATGGSAPAC PLLLADQGPA GPKGKEPVVY PWMKKIHVSA VNSSYNGGEP
KRSRTAYTRQ QVLELEKEFH FNRYLTRRRR IEIAHTLCLS ERQVKIWFQN RRMKWKKDHK
LPNTKMRSSN TASAPAGPPG KAQTHSPHHH PHPLPGASTP IPSSI
