HXB2_HUMAN
ID HXB2_HUMAN Reviewed; 356 AA.
AC P14652; P10913; P17485;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 196.
DE RecName: Full=Homeobox protein Hox-B2;
DE AltName: Full=Homeobox protein Hox-2.8;
DE AltName: Full=Homeobox protein Hox-2H;
DE AltName: Full=K8;
GN Name=HOXB2; Synonyms=HOX2H;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2574852; DOI=10.1093/nar/17.24.10385;
RA Acampora D., D'Esposito M., Faiella A., Pannese M., Migliaccio E.,
RA Morelli F., Stornaiuolo A., Nigro V., Simeone A., Boncinelli E.;
RT "The human HOX gene family.";
RL Nucleic Acids Res. 17:10385-10402(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-208.
RC TISSUE=Placenta;
RX PubMed=2570724; DOI=10.1111/j.1432-0436.1989.tb00598.x;
RA Giampaolo A., Acampora D., Zappavigna V., Pannese M., D'Esposito M.,
RA Care A., Faiella A., Stornaiuolo A., Russo G., Simeone A., Boncinelli E.,
RA Peschle C.;
RT "Differential expression of human HOX-2 genes along the anterior-posterior
RT axis in embryonic central nervous system.";
RL Differentiation 40:191-197(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-208.
RX PubMed=2576652; DOI=10.1139/g89-133;
RA Boncinelli E., Acampora D., Pannese M., D'Esposito M., Somma R.,
RA Gaudino G., Stornaiuolo A., Cafiero M., Faiella A., Simeone A.;
RT "Organization of human class I homeobox genes.";
RL Genome 31:745-756(1989).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 143-202.
RX PubMed=2901346; DOI=10.1002/j.1460-2075.1988.tb03052.x;
RA Kongsuwan K., Webb E., Housiaux P., Adams J.M.;
RT "Expression of multiple homeobox genes within diverse mammalian
RT haemopoietic lineages.";
RL EMBO J. 7:2131-2138(1988).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RX PubMed=7876223; DOI=10.1074/jbc.270.9.4544;
RA Vieille-Grosjean I., Huber P.;
RT "Transcription factor GATA-1 regulates human HOXB2 gene expression in
RT erythroid cells.";
RL J. Biol. Chem. 270:4544-4550(1995).
RN [7]
RP FUNCTION, AND GAMMA-GLOBIN PROMOTER AND ENHANCER BINDING FACTOR COMPLEX.
RX PubMed=10595394; DOI=10.1089/104454999314809;
RA Case S.S., Huber P., Lloyd J.A.;
RT "The gammaPE complex contains both SATB1 and HOXB2 and has positive and
RT negative roles in human gamma-globin gene regulation.";
RL DNA Cell Biol. 18:805-817(1999).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC developmental regulatory system that provides cells with specific
CC positional identities on the anterior-posterior axis.
CC {ECO:0000269|PubMed:10595394}.
CC -!- SUBUNIT: Part of the nuclear protein complex gamma-globin promoter and
CC enhancer binding factor (gamma-PE) composed at least by SATB1 and
CC HOXB2.
CC -!- INTERACTION:
CC P14652; Q86UW9: DTX2; NbExp=3; IntAct=EBI-5329558, EBI-740376;
CC P14652; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-5329558, EBI-10172526;
CC P14652; Q6PF18: MORN3; NbExp=3; IntAct=EBI-5329558, EBI-9675802;
CC P14652; Q9UHB4: NDOR1; NbExp=3; IntAct=EBI-5329558, EBI-10249760;
CC P14652; Q9BYU1: PBX4; NbExp=3; IntAct=EBI-5329558, EBI-10302990;
CC P14652; Q06455-2: RUNX1T1; NbExp=3; IntAct=EBI-5329558, EBI-11984663;
CC P14652; Q9H2K2: TNKS2; NbExp=3; IntAct=EBI-5329558, EBI-4398527;
CC P14652; Q8TF42: UBASH3B; NbExp=3; IntAct=EBI-5329558, EBI-1380492;
CC P14652; Q86VK4-3: ZNF410; NbExp=3; IntAct=EBI-5329558, EBI-11741890;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed in whole embryos and fetuses at 5-9
CC weeks from conception.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. Proboscipedia
CC subfamily. {ECO:0000305}.
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DR EMBL; X16665; CAA34655.1; -; mRNA.
DR EMBL; BC074805; AAH74805.1; -; mRNA.
DR EMBL; BC074806; AAH74806.1; -; mRNA.
DR EMBL; X16176; CAA34298.1; -; Genomic_DNA.
DR EMBL; X14571; CAA32709.1; -; mRNA.
DR EMBL; X78978; CAA55581.1; -; Genomic_DNA.
DR CCDS; CCDS11527.1; -.
DR PIR; S07542; WJHU2H.
DR RefSeq; NP_002136.1; NM_002145.3.
DR AlphaFoldDB; P14652; -.
DR SMR; P14652; -.
DR BioGRID; 109452; 15.
DR ELM; P14652; -.
DR IntAct; P14652; 30.
DR STRING; 9606.ENSP00000331741; -.
DR iPTMnet; P14652; -.
DR PhosphoSitePlus; P14652; -.
DR BioMuta; HOXB2; -.
DR DMDM; 123270; -.
DR MassIVE; P14652; -.
DR PaxDb; P14652; -.
DR PeptideAtlas; P14652; -.
DR PRIDE; P14652; -.
DR Antibodypedia; 30263; 171 antibodies from 27 providers.
DR DNASU; 3212; -.
DR Ensembl; ENST00000330070.6; ENSP00000331741.4; ENSG00000173917.11.
DR GeneID; 3212; -.
DR KEGG; hsa:3212; -.
DR MANE-Select; ENST00000330070.6; ENSP00000331741.4; NM_002145.4; NP_002136.1.
DR UCSC; uc002inm.5; human.
DR CTD; 3212; -.
DR DisGeNET; 3212; -.
DR GeneCards; HOXB2; -.
DR HGNC; HGNC:5113; HOXB2.
DR HPA; ENSG00000173917; Low tissue specificity.
DR MIM; 142967; gene.
DR neXtProt; NX_P14652; -.
DR OpenTargets; ENSG00000173917; -.
DR PharmGKB; PA29389; -.
DR VEuPathDB; HostDB:ENSG00000173917; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000155029; -.
DR HOGENOM; CLU_048378_0_0_1; -.
DR InParanoid; P14652; -.
DR OMA; EDICDPA; -.
DR OrthoDB; 835724at2759; -.
DR PhylomeDB; P14652; -.
DR TreeFam; TF317730; -.
DR PathwayCommons; P14652; -.
DR Reactome; R-HSA-5617472; Activation of anterior HOX genes in hindbrain development during early embryogenesis.
DR SignaLink; P14652; -.
DR SIGNOR; P14652; -.
DR BioGRID-ORCS; 3212; 8 hits in 1098 CRISPR screens.
DR ChiTaRS; HOXB2; human.
DR GeneWiki; HOXB2; -.
DR GenomeRNAi; 3212; -.
DR Pharos; P14652; Tbio.
DR PRO; PR:P14652; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; P14652; protein.
DR Bgee; ENSG00000173917; Expressed in seminal vesicle and 149 other tissues.
DR Genevisible; P14652; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IEA:Ensembl.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IEA:Ensembl.
DR GO; GO:0048704; P:embryonic skeletal system morphogenesis; IEA:Ensembl.
DR GO; GO:0021612; P:facial nerve structural organization; IEA:Ensembl.
DR GO; GO:0002011; P:morphogenesis of an epithelial sheet; IEA:Ensembl.
DR GO; GO:0007399; P:nervous system development; NAS:UniProtKB.
DR GO; GO:0048857; P:neural nucleus development; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0021569; P:rhombomere 3 development; IEA:Ensembl.
DR GO; GO:0021570; P:rhombomere 4 development; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00032; ANTENNAPEDIA; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..356
FT /note="Homeobox protein Hox-B2"
FT /id="PRO_0000200114"
FT DNA_BIND 143..202
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 39..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 94..99
FT /note="Antp-type hexapeptide"
FT COMPBIAS 75..94
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 274
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CONFLICT 136..138
FT /note="PEA -> RRL (in Ref. 3; CAA34298)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 356 AA; 37914 MW; D7ACA0E3D5BEBFB9 CRC64;
MNFEFEREIG FINSQPSLAE CLTSFPAVLE TFQTSSIKES TLIPPPPPFE QTFPSLQPGA
STLQRPRSQK RAEDGPALPP PPPPPLPAAP PAPEFPWMKE KKSAKKPSQS ATSPSPAASA
VPASGVGSPA DGLGLPEAGG GGARRLRTAY TNTQLLELEK EFHFNKYLCR PRRVEIAALL
DLTERQVKVW FQNRRMKHKR QTQHREPPDG EPACPGALED ICDPAEEPAA SPGGPSASRA
AWEACCHPPE VVPGALSADP RPLAVRLEGA GASSPGCALR GAGGLEPGPL PEDVFSGRQD
SPFLPDLNFF AADSCLQLSG GLSPSLQGSL DSPVPFSEEE LDFFTSTLCA IDLQFP
