HXB4_HUMAN
ID HXB4_HUMAN Reviewed; 251 AA.
AC P17483; Q9NTA0;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 03-AUG-2022, entry version 201.
DE RecName: Full=Homeobox protein Hox-B4;
DE AltName: Full=Homeobox protein Hox-2.6;
DE AltName: Full=Homeobox protein Hox-2F;
GN Name=HOXB4; Synonyms=HOX2F;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1981366; DOI=10.1111/j.1432-0436.1990.tb00458.x;
RA Peverali F.A., D'Esposito M., Acampora D., Bunone G., Negri M., Faiella A.,
RA Stornaiuolo A., Pannese M., Migliaccio E., Simeone A., Valle G.D.,
RA Boncinelli E.;
RT "Expression of HOX homeogenes in human neuroblastoma cell culture lines.";
RL Differentiation 45:61-69(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kidd K.K., Busygina V., DeMille M.M.C., Speed W.C., Ruggeri V., Kidd J.R.,
RA Pakstis A.J.;
RT "Overall linkage disequilibrium in 33 populations for highly informative
RT multisite haplotypes spanning the HOXB gene cluster.";
RL Am. J. Hum. Genet. 67:235-235(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11085749; DOI=10.1084/jem.192.10.1479;
RA Giannola D.M., Shlomchik W.D., Jegathesan M., Liebowitz D., Abrams C.S.,
RA Kadesch T., Dancis A., Emerson S.G.;
RT "Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem
RT cells through transcriptional activation of the HOXB4 promoter by upstream
RT stimulating factor (USF)-1 and USF-2.";
RL J. Exp. Med. 192:1479-1490(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-251.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE OF 160-227.
RC TISSUE=Placenta;
RX PubMed=2570724; DOI=10.1111/j.1432-0436.1989.tb00598.x;
RA Giampaolo A., Acampora D., Zappavigna V., Pannese M., D'Esposito M.,
RA Care A., Faiella A., Stornaiuolo A., Russo G., Simeone A., Boncinelli E.,
RA Peschle C.;
RT "Differential expression of human HOX-2 genes along the anterior-posterior
RT axis in embryonic central nervous system.";
RL Differentiation 40:191-197(1989).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-227.
RX PubMed=2576652; DOI=10.1139/g89-133;
RA Boncinelli E., Acampora D., Pannese M., D'Esposito M., Somma R.,
RA Gaudino G., Stornaiuolo A., Cafiero M., Faiella A., Simeone A.;
RT "Organization of human class I homeobox genes.";
RL Genome 31:745-756(1989).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC developmental regulatory system that provides cells with specific
CC positional identities on the anterior-posterior axis.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed in whole embryos and fetuses at 5-9
CC weeks from conception.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily.
CC {ECO:0000305}.
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DR EMBL; X16174; CAA34296.1; -; Genomic_DNA.
DR EMBL; AF287967; AAG31554.1; -; Genomic_DNA.
DR EMBL; AF307160; AAG45052.1; -; Genomic_DNA.
DR EMBL; BC049204; AAH49204.1; -; mRNA.
DR EMBL; AL137449; CAB70742.1; -; mRNA.
DR CCDS; CCDS11529.1; -.
DR PIR; B60492; B60492.
DR PIR; T46446; T46446.
DR RefSeq; NP_076920.1; NM_024015.4.
DR AlphaFoldDB; P17483; -.
DR SMR; P17483; -.
DR BioGRID; 109454; 5.
DR ELM; P17483; -.
DR STRING; 9606.ENSP00000328928; -.
DR iPTMnet; P17483; -.
DR PhosphoSitePlus; P17483; -.
DR BioMuta; HOXB4; -.
DR DMDM; 547692; -.
DR EPD; P17483; -.
DR MassIVE; P17483; -.
DR MaxQB; P17483; -.
DR PaxDb; P17483; -.
DR PeptideAtlas; P17483; -.
DR PRIDE; P17483; -.
DR ProteomicsDB; 53477; -.
DR Antibodypedia; 17849; 318 antibodies from 35 providers.
DR DNASU; 3214; -.
DR Ensembl; ENST00000332503.6; ENSP00000328928.5; ENSG00000182742.6.
DR GeneID; 3214; -.
DR KEGG; hsa:3214; -.
DR MANE-Select; ENST00000332503.6; ENSP00000328928.5; NM_024015.5; NP_076920.1.
DR UCSC; uc002inp.4; human.
DR CTD; 3214; -.
DR DisGeNET; 3214; -.
DR GeneCards; HOXB4; -.
DR HGNC; HGNC:5115; HOXB4.
DR HPA; ENSG00000182742; Tissue enhanced (epididymis).
DR MIM; 142965; gene.
DR neXtProt; NX_P17483; -.
DR OpenTargets; ENSG00000182742; -.
DR PharmGKB; PA29391; -.
DR VEuPathDB; HostDB:ENSG00000182742; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000162072; -.
DR HOGENOM; CLU_061398_0_0_1; -.
DR InParanoid; P17483; -.
DR OMA; FGRRSAC; -.
DR OrthoDB; 1154159at2759; -.
DR PhylomeDB; P17483; -.
DR TreeFam; TF352857; -.
DR PathwayCommons; P17483; -.
DR Reactome; R-HSA-5617472; Activation of anterior HOX genes in hindbrain development during early embryogenesis.
DR SignaLink; P17483; -.
DR SIGNOR; P17483; -.
DR BioGRID-ORCS; 3214; 18 hits in 1105 CRISPR screens.
DR GeneWiki; HOXB4; -.
DR GenomeRNAi; 3214; -.
DR Pharos; P17483; Tbio.
DR PRO; PR:P17483; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; P17483; protein.
DR Bgee; ENSG00000182742; Expressed in right uterine tube and 79 other tissues.
DR Genevisible; P17483; HS.
DR GO; GO:0005813; C:centrosome; IDA:HPA.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0048539; P:bone marrow development; IEA:Ensembl.
DR GO; GO:0060216; P:definitive hemopoiesis; IEA:Ensembl.
DR GO; GO:0048704; P:embryonic skeletal system morphogenesis; IBA:GO_Central.
DR GO; GO:0060218; P:hematopoietic stem cell differentiation; IDA:BHF-UCL.
DR GO; GO:0071425; P:hematopoietic stem cell proliferation; IEA:Ensembl.
DR GO; GO:0002011; P:morphogenesis of an epithelial sheet; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:2000738; P:positive regulation of stem cell differentiation; IDA:BHF-UCL.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:BHF-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048103; P:somatic stem cell division; IEA:Ensembl.
DR GO; GO:0048536; P:spleen development; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017995; Homeobox_antennapedia.
DR InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00025; ANTENNAPEDIA.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00032; ANTENNAPEDIA; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..251
FT /note="Homeobox protein Hox-B4"
FT /id="PRO_0000200122"
FT DNA_BIND 162..221
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 220..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 141..146
FT /note="Antp-type hexapeptide"
FT COMPBIAS 69..103
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..127
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 90
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
SQ SEQUENCE 251 AA; 27604 MW; 23EE1D0DDCCE2DB4 CRC64;
MAMSSFLINS NYVDPKFPPC EEYSQSDYLP SDHSPGYYAG GQRRESSFQP EAGFGRRAAC
TVQRYAACRD PGPPPPPPPP PPPPPPPGLS PRAPAPPPAG ALLPEPGQRC EAVSSSPPPP
PCAQNPLHPS PSHSACKEPV VYPWMRKVHV STVNPNYAGG EPKRSRTAYT RQQVLELEKE
FHYNRYLTRR RRVEIAHALC LSERQIKIWF QNRRMKWKKD HKLPNTKIRS GGAAGSAGGP
PGRPNGGPRA L
