ID   HXB5A_DANRE             Reviewed;         275 AA.
AC   P09014; Q4PRA3; Q6LBU7; Q8AWY7;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 2.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=Homeobox protein Hox-B5a;
DE            Short=Hox-B5;
DE   AltName: Full=Homeobox protein Zf-21;
GN   Name=hoxb5a; Synonyms=hox-b5, hoxb5, zf21;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC   TISSUE=Embryo;
RX   PubMed=2902580; DOI=10.1093/nar/16.19.9097;
RA   Njolstad P.R., Molven A., Hordvik I., Apold J., Fjose A.;
RT   "Primary structure, developmentally regulated expression and potential
RT   duplication of the zebrafish homeobox gene ZF-21.";
RL   Nucleic Acids Res. 16:9097-9111(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8097929; DOI=10.1016/0167-4781(93)90253-a;
RA   Molven A., Hordvik I., Njolstad P.R.;
RT   "Sequence analysis of the zebrafish hox-B5/B6 region.";
RL   Biochim. Biophys. Acta 1173:102-106(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 113-201.
RC   STRAIN=Tuebingen;
RX   PubMed=16174031; DOI=10.1111/j.1525-142x.2005.05042.x;
RA   Corredor-Adamez M., Welten M.C.M., Spaink H.P., Jeffery J.E., Schoon R.T.,
RA   de Bakker M.A.G., Bagowski C.P., Meijer A.H., Verbeek F.J.,
RA   Richardson M.K.;
RT   "Genomic annotation and transcriptome analysis of the zebrafish (Danio
RT   rerio) hox complex with description of a novel member, hoxb13a.";
RL   Evol. Dev. 7:362-375(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 195-275.
RX   PubMed=2895022; DOI=10.1016/0014-5793(88)80634-3;
RA   Njolstad P.R., Molven A., Fjose A.;
RT   "A zebrafish homologue of the murine Hox-2.1 gene.";
RL   FEBS Lett. 230:25-30(1988).
RN   [7]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=9425136; DOI=10.1242/dev.125.3.407;
RA   Prince V.E., Joly L., Ekker M., Ho R.K.;
RT   "Zebrafish hox genes: genomic organization and modified colinear expression
RT   patterns in the trunk.";
RL   Development 125:407-420(1998).
CC   -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC       developmental regulatory system that provides cells with specific
CC       positional identities on the anterior-posterior axis.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DEVELOPMENTAL STAGE: First detected at 12.5 hours post-fertilization,
CC       continuing through to adult stages. At the 10-somite stage, expressed
CC       in the paraxial mesoderm with an anterior expression at somite 1. At
CC       the 20-somite stage, expressed within the developing CNS with an
CC       anterior expression limit at the anterior-most region of somite 1.
CC       {ECO:0000269|PubMed:2902580, ECO:0000269|PubMed:9425136}.
CC   -!- SIMILARITY: Belongs to the Antp homeobox family. {ECO:0000305}.
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DR   EMBL; X12802; CAA31290.1; -; mRNA.
DR   EMBL; X68248; CAA48320.1; -; Genomic_DNA.
DR   EMBL; AL645782; CAD59114.1; -; Genomic_DNA.
DR   EMBL; BC071493; AAH71493.1; -; mRNA.
DR   EMBL; DQ060540; AAY67918.1; -; mRNA.
DR   EMBL; X07381; CAA30293.1; -; Genomic_DNA.
DR   EMBL; X07381; CAA30294.1; -; Genomic_DNA.
DR   PIR; S03671; WJZFX2.
DR   RefSeq; NP_571176.2; NM_131101.2.
DR   AlphaFoldDB; P09014; -.
DR   SMR; P09014; -.
DR   STRING; 7955.ENSDARP00000004270; -.
DR   PaxDb; P09014; -.
DR   Ensembl; ENSDART00000025449; ENSDARP00000004270; ENSDARG00000013057.
DR   GeneID; 30317; -.
DR   KEGG; dre:30317; -.
DR   CTD; 30317; -.
DR   ZFIN; ZDB-GENE-980526-70; hoxb5a.
DR   eggNOG; KOG0489; Eukaryota.
DR   GeneTree; ENSGT00940000158354; -.
DR   HOGENOM; CLU_061398_2_1_1; -.
DR   InParanoid; P09014; -.
DR   OMA; TEGQTPQ; -.
DR   OrthoDB; 980936at2759; -.
DR   PhylomeDB; P09014; -.
DR   TreeFam; TF316310; -.
DR   PRO; PR:P09014; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 3.
DR   Bgee; ENSDARG00000013057; Expressed in spleen and 32 other tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR   GO; GO:0007420; P:brain development; IMP:ZFIN.
DR   GO; GO:0001708; P:cell fate specification; IMP:ZFIN.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017995; Homeobox_antennapedia.
DR   InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00025; ANTENNAPEDIA.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00032; ANTENNAPEDIA; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..275
FT                   /note="Homeobox protein Hox-B5a"
FT                   /id="PRO_0000200132"
FT   DNA_BIND        200..259
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          70..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           182..187
FT                   /note="Antp-type hexapeptide"
FT   COMPBIAS        76..138
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..179
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        107..114
FT                   /note="LGPKGSSP -> FGTQRLFA (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   275 AA;  30558 MW;  E8C4586BCEFA3AD4 CRC64;
     MSSYFVNSFS GRYPNGPDYQ LLNYGTSSSA MNASYRDSGT MHSGSYGYNY NGMDLSVNRS
     TSTGHFGAVG DNSRVFQSPA PETRFRQPSS CSLASPEPLP CSNSESLGPK GSSPPSDQST
     TTAGNNLNSN THFTEIDEAS ASSETEEASH RANNSAPRTQ QKQETTATST TSATSDGQAP
     QIFPWMRKLH ISHDMTGPDG KRARTAYTRY QTLELEKEFH FNRYLTRRRR IEIAHALCLS
     ERQIKIWFQN RRMKWKKDNK LKSMSLATAG SAFQP