ID   APAH_PSEAE              Reviewed;         283 AA.
AC   Q9I5U7;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Bis(5'-nucleosyl)-tetraphosphatase, symmetrical;
DE            EC=3.6.1.41;
DE   AltName: Full=Ap4A hydrolase;
DE   AltName: Full=Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase;
DE   AltName: Full=Diadenosine tetraphosphatase;
GN   Name=apaH; OrderedLocusNames=PA0590;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield
CC       ADP. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2
CC         H(+); Xref=Rhea:RHEA:24252, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58141, ChEBI:CHEBI:456216; EC=3.6.1.41;
CC   -!- SIMILARITY: Belongs to the Ap4A hydrolase family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG03979.1; -; Genomic_DNA.
DR   PIR; F83571; F83571.
DR   RefSeq; NP_249281.1; NC_002516.2.
DR   RefSeq; WP_003113213.1; NZ_QZGE01000010.1.
DR   AlphaFoldDB; Q9I5U7; -.
DR   SMR; Q9I5U7; -.
DR   STRING; 287.DR97_3559; -.
DR   PaxDb; Q9I5U7; -.
DR   PRIDE; Q9I5U7; -.
DR   DNASU; 880834; -.
DR   EnsemblBacteria; AAG03979; AAG03979; PA0590.
DR   GeneID; 880834; -.
DR   KEGG; pae:PA0590; -.
DR   PATRIC; fig|208964.12.peg.626; -.
DR   PseudoCAP; PA0590; -.
DR   HOGENOM; CLU_056184_2_0_6; -.
DR   InParanoid; Q9I5U7; -.
DR   OMA; INAFTRM; -.
DR   PhylomeDB; Q9I5U7; -.
DR   BioCyc; PAER208964:G1FZ6-597-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0008803; F:bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity; IBA:GO_Central.
DR   GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR   GO; GO:0110154; P:RNA decapping; IBA:GO_Central.
DR   CDD; cd07422; MPP_ApaH; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   HAMAP; MF_00199; ApaH; 1.
DR   InterPro; IPR004617; ApaH.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF00149; Metallophos; 1.
DR   PIRSF; PIRSF000903; B5n-ttraPtase_sm; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
DR   TIGRFAMs; TIGR00668; apaH; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..283
FT                   /note="Bis(5'-nucleosyl)-tetraphosphatase, symmetrical"
FT                   /id="PRO_0000198004"
SQ   SEQUENCE   283 AA;  32019 MW;  B7F21AE147168459 CRC64;
     MAVYAVGDLQ GCLDPLKCLL ERVAFDPAKD RLWLVGDLVN RGPQSLETLR FLYAMRESVV
     SVLGNHDLHL LAVAHKSERL KKSDTLREIL EAPDREPLLD WLRRLPLLHY DEQRKVALVH
     AGIPPQWSLE KARLRAAEVE QALRDDQRLP LFLDGMYGNE PAKWDKKLHG IDRLRVITNY
     FTRMRFCTED GKLDLKSKEG LDTAPPGYAP WFSFPSRKTR GEKIIFGHWA ALEGHCDEPG
     LFALDTGCVW GARMTLLNVD SGERLSCDCA EQRAPARPAA TPA