HXD13_MOUSE
ID HXD13_MOUSE Reviewed; 339 AA.
AC P70217; A2ASM5; Q2VPB0; Q2VPB1; Q64177;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Homeobox protein Hox-D13;
DE AltName: Full=Homeobox protein Hox-4.8;
GN Name=Hoxd13; Synonyms=Hox-4.8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8978698; DOI=10.1002/j.1460-2075.1996.tb01062.x;
RA Herault Y., Hraba-Reveney S., van der Hoeven F., Duboule D.;
RT "Function of the Evx-2 gene in the morphogenesis of vertebrate limbs.";
RL EMBO J. 15:6727-6738(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-339.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 258-339.
RX PubMed=1685889; DOI=10.1016/0925-4773(91)90067-g;
RA Dolle P., Izpisua-Belmonte J.-C., Boncinelli E., Duboule D.;
RT "The Hox-4.8 gene is localized at the 5' extremity of the Hox-4 complex and
RT is expressed in the most posterior parts of the body during development.";
RL Mech. Dev. 36:3-13(1991).
RN [5]
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLN-321.
RX PubMed=23995701; DOI=10.1101/gr.157610.113;
RA Ibrahim D.M., Hansen P., Roedelsperger C., Stiege A.C., Doelken S.C.,
RA Horn D., Jaeger M., Janetzki C., Krawitz P., Leschik G., Wagner F.,
RA Scheuer T., Schmidt-von Kegler M., Seemann P., Timmermann B.,
RA Robinson P.N., Mundlos S., Hecht J.;
RT "Distinct global shifts in genomic binding profiles of limb malformation-
RT associated HOXD13 mutations.";
RL Genome Res. 23:2091-2102(2013).
CC -!- FUNCTION: Sequence-specific transcription factor that binds gene
CC promoters and activates their transcription (PubMed:23995701). Part of
CC a developmental regulatory system that provides cells with specific
CC positional identities on the anterior-posterior axis (By similarity).
CC {ECO:0000250|UniProtKB:P24344, ECO:0000269|PubMed:23995701}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:15489334}.
CC -!- SIMILARITY: Belongs to the Abd-B homeobox family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC {ECO:0000305}.
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DR EMBL; X99291; CAA67675.1; -; mRNA.
DR EMBL; AL928644; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC109143; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC109144; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; S80553; AAB21366.2; -; Genomic_DNA.
DR CCDS; CCDS16139.1; -.
DR PIR; A56563; A56563.
DR RefSeq; NP_032301.2; NM_008275.3.
DR AlphaFoldDB; P70217; -.
DR SMR; P70217; -.
DR BioGRID; 200395; 7.
DR IntAct; P70217; 7.
DR MINT; P70217; -.
DR STRING; 10090.ENSMUSP00000001872; -.
DR PhosphoSitePlus; P70217; -.
DR MaxQB; P70217; -.
DR PaxDb; P70217; -.
DR PRIDE; P70217; -.
DR ProteomicsDB; 267027; -.
DR Antibodypedia; 33902; 250 antibodies from 32 providers.
DR DNASU; 15433; -.
DR Ensembl; ENSMUST00000001872; ENSMUSP00000001872; ENSMUSG00000001819.
DR GeneID; 15433; -.
DR KEGG; mmu:15433; -.
DR UCSC; uc008kdu.2; mouse.
DR CTD; 3239; -.
DR MGI; MGI:96205; Hoxd13.
DR VEuPathDB; HostDB:ENSMUSG00000001819; -.
DR eggNOG; KOG0487; Eukaryota.
DR GeneTree; ENSGT00940000161457; -.
DR HOGENOM; CLU_059940_2_0_1; -.
DR InParanoid; P70217; -.
DR OMA; KECPAPA; -.
DR OrthoDB; 1190384at2759; -.
DR PhylomeDB; P70217; -.
DR TreeFam; TF330813; -.
DR BioGRID-ORCS; 15433; 4 hits in 75 CRISPR screens.
DR PRO; PR:P70217; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P70217; protein.
DR Bgee; ENSMUSG00000001819; Expressed in undifferentiated genital tubercle and 128 other tissues.
DR Genevisible; P70217; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0060602; P:branch elongation of an epithelium; IMP:MGI.
DR GO; GO:0042733; P:embryonic digit morphogenesis; IGI:MGI.
DR GO; GO:0048619; P:embryonic hindgut morphogenesis; ISO:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:MGI.
DR GO; GO:0022612; P:gland morphogenesis; IMP:MGI.
DR GO; GO:0035108; P:limb morphogenesis; IMP:MGI.
DR GO; GO:0030539; P:male genitalia development; IMP:MGI.
DR GO; GO:0060571; P:morphogenesis of an epithelial fold; IMP:MGI.
DR GO; GO:0007389; P:pattern specification process; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0060527; P:prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis; IGI:MGI.
DR GO; GO:0060687; P:regulation of branching involved in prostate gland morphogenesis; IMP:MGI.
DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0033574; P:response to testosterone; IEA:Ensembl.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR022067; HoxA13_N.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF12284; HoxA13_N; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..339
FT /note="Homeobox protein Hox-D13"
FT /id="PRO_0000200245"
FT DNA_BIND 272..331
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 321
FT /note="Q->K: Changes the specificity for binding motifs in
FT promoters. Recognizes the PITX1 consensus DNA-binding sites
FT and triggers the expression of a gene pattern similar to
FT PITX1."
FT /evidence="ECO:0000269|PubMed:23995701"
FT MUTAGEN 321
FT /note="Q->R: Changes the function in transcription
FT regulation."
FT /evidence="ECO:0000269|PubMed:23995701"
FT CONFLICT 9
FT /note="M -> I (in Ref. 3; BC109143)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="S -> T (in Ref. 1; CAA67675)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="R -> T (in Ref. 4; AAB21366)"
FT /evidence="ECO:0000305"
FT CONFLICT 307
FT /note="A -> R (in Ref. 4; AAB21366)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 339 AA; 35889 MW; C1BD2FAF6D56E533 CRC64;
MSRSGTWDMD GLRADGGAAG AAPASSSSSV AAPGQCRGFL SAPVFAGTHS GRAAAAAAAA
AAAAAAASSF AYPGTSERTG SSSSSSSSAV IATRPEAPVA KECPAPAAAA TAAAPPGAPA
LGYGYHFGNG YYSCRMSHGV GLQQNALKSS PHASLGGFPV EKYMDVSGLA SSSVPTNEVP
ARAKEVSFYQ GYTSPYQHVP GYIDMVSTFG SGEPRHEAYI SMEGYQSWTL ANGWNSQVYC
AKDQPQGSHF WKSSFPGDVA LNQPDMCVYR RGRKKRVPYT KLQLKELENE YAINKFINKD
KRRRISAATN LSERQVTIWF QNRRVKDKKI VSKLKDTVS
