HXK10_ORYSJ
ID HXK10_ORYSJ Reviewed; 504 AA.
AC Q2KNB5; A0A0N7KKN6; Q75K74;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Hexokinase-10;
DE EC=2.7.1.1 {ECO:0000305|PubMed:16552590};
DE AltName: Full=Hexokinase-7;
GN Name=HXK10; Synonyms=HXK7; OrderedLocusNames=Os05g0375100, LOC_Os05g31110;
GN ORFNames=OJ1005_E12.7;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND
RP NOMENCLATURE.
RC STRAIN=cv. Jinmi;
RX PubMed=16552590; DOI=10.1007/s00425-006-0251-y;
RA Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H.,
RA Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S.;
RT "Structure, expression, and functional analysis of the hexokinase gene
RT family in rice (Oryza sativa L.).";
RL Planta 224:598-611(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Zhonghua 15; TISSUE=Flower;
RA Wang Y.D., Cheng W., Wang X.S., Zhou X.J.;
RT "The hexokinase gene family in rice.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme.
CC {ECO:0000269|PubMed:16552590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000305|PubMed:16552590}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000305|PubMed:16552590}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in stamen.
CC {ECO:0000269|PubMed:16552590}.
CC -!- MISCELLANEOUS: Expression may pollen-specific.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT01343.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ116392; AAZ93627.1; -; mRNA.
DR EMBL; AY884170; AAX68423.1; -; mRNA.
DR EMBL; AC108874; AAT01343.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; BAS93721.1; -; Genomic_DNA.
DR RefSeq; XP_015638932.1; XM_015783446.1.
DR AlphaFoldDB; Q2KNB5; -.
DR SMR; Q2KNB5; -.
DR STRING; 4530.OS05T0375100-00; -.
DR PaxDb; Q2KNB5; -.
DR PRIDE; Q2KNB5; -.
DR EnsemblPlants; Os05t0375100-00; Os05t0375100-00; Os05g0375100.
DR GeneID; 107275630; -.
DR Gramene; Os05t0375100-00; Os05t0375100-00; Os05g0375100.
DR KEGG; osa:107275630; -.
DR eggNOG; KOG1369; Eukaryota.
DR HOGENOM; CLU_014393_5_1_1; -.
DR InParanoid; Q2KNB5; -.
DR OMA; FMAINCE; -.
DR OrthoDB; 1153545at2759; -.
DR BRENDA; 2.7.1.1; 4460.
DR PlantReactome; R-OSA-1119570; Cytosolic glycolysis.
DR PlantReactome; R-OSA-1119595; Mannose degradation.
DR PlantReactome; R-OSA-1119601; Trehalose degradation II.
DR PlantReactome; R-OSA-1119628; GDP-mannose metabolism.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q2KNB5; OS.
DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IEA:RHEA.
DR GO; GO:0004340; F:glucokinase activity; IEA:UniProt.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0001678; P:cellular glucose homeostasis; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00378; HEXOKINASE_1; 1.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Glycolysis; Kinase; Membrane; Nucleotide-binding;
KW Plastid; Plastid outer membrane; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..504
FT /note="Hexokinase-10"
FT /id="PRO_0000247573"
FT TRANSMEM 7..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 39..493
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 94..226
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 169..195
FT /note="Glucose-binding"
FT /evidence="ECO:0000255"
FT REGION 227..482
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT BINDING 105..110
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 504 AA; 54507 MW; 8DA351554956D7C9 CRC64;
MEGRAAGWVR VAAVGWAVAA CAVAAGMVAR RGAARVRWNR AVAVVRDLEE RCATPAELLQ
RVVNSLAIEM FAGLASDGGS KVRMLLTCVD ALPDGSEEGI SYAIDLGGTS FRVLKVELGA
GSTIINRKVE HQPIPENLTK GTSDDLFNFI ASALKNFIER EGGEVEGRAL GFTFSFPVRQ
TSISSGTLIR WTKEFSIEEA VGKDVAQCLN EALARNGLNM KVNVLVNNTV GTLALGHYYD
DDTVAAVIIG AGTNACYIER NDAIIKSLGR VTNSERTVVN VEWGSFRPPQ IELTPYDICF
NNETWNYYDQ GFEKMISGVY LGEIARLVFQ KMAEESDIFG TAVDGLSTPF VLSTPNLAAI
REDDSPDLRE VGKILEEHLK LPDVPLKTRK LVARVSDIIT RRAARLAAAA IVAILQKIGC
DGTLCGSTQV RTMRGVRRRT VVAIEGGLFE GYSVFREYLN EALVEILGEE IAATVSLRVM
EEGSGTGAAL LAAAYSSARQ KNSE
