HXK1_ORYSJ
ID HXK1_ORYSJ Reviewed; 498 AA.
AC Q8LH82; Q0D6S1;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Hexokinase-1;
DE EC=2.7.1.1 {ECO:0000305|PubMed:16552590};
GN Name=HXK1; OrderedLocusNames=Os07g0446800, LOC_Os07g26540;
GN ORFNames=P0030H06.133, P0475E07.113;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND NOMENCLATURE.
RC STRAIN=cv. Jinmi;
RX PubMed=16552590; DOI=10.1007/s00425-006-0251-y;
RA Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H.,
RA Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S.;
RT "Structure, expression, and functional analysis of the hexokinase gene
RT family in rice (Oryza sativa L.).";
RL Planta 224:598-611(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Zhonghua 15; TISSUE=Flower;
RA Wang Y.D., Cheng W., Wang X.S., Zhou X.J.;
RT "The hexokinase gene family in rice.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme.
CC {ECO:0000269|PubMed:16552590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000305|PubMed:16552590}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000305|PubMed:16552590}.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
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DR EMBL; DQ116383; AAZ93618.1; -; mRNA.
DR EMBL; AY884164; AAX68417.1; -; mRNA.
DR EMBL; AP004395; BAC10209.1; -; Genomic_DNA.
DR EMBL; AP004668; BAC16101.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21452.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT01294.1; -; Genomic_DNA.
DR RefSeq; XP_015645221.1; XM_015789735.1.
DR AlphaFoldDB; Q8LH82; -.
DR SMR; Q8LH82; -.
DR STRING; 4530.OS07T0446800-00; -.
DR PaxDb; Q8LH82; -.
DR PRIDE; Q8LH82; -.
DR EnsemblPlants; Os07t0446800-00; Os07t0446800-00; Os07g0446800.
DR GeneID; 4343113; -.
DR Gramene; Os07t0446800-00; Os07t0446800-00; Os07g0446800.
DR KEGG; osa:4343113; -.
DR eggNOG; KOG1369; Eukaryota.
DR HOGENOM; CLU_014393_5_1_1; -.
DR InParanoid; Q8LH82; -.
DR OMA; TEWGSFA; -.
DR OrthoDB; 1153545at2759; -.
DR BRENDA; 2.7.1.1; 4460.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q8LH82; OS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR GO; GO:0004340; F:glucokinase activity; IBA:GO_Central.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR GO; GO:0001678; P:cellular glucose homeostasis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Glycolysis; Kinase; Nucleotide-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..498
FT /note="Hexokinase-1"
FT /id="PRO_0000247564"
FT DOMAIN 39..492
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 95..233
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 176..202
FT /note="Glucose-binding"
FT /evidence="ECO:0000255"
FT REGION 234..481
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT BINDING 106..111
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 498 AA; 51772 MW; 6DC7BD48B62C985E CRC64;
MAAAAVAADQ KVVTMTSLRE GCACAAPPAA AAPPMPKMAA AQRVVAELRE ACATPAARLA
EVAAAMAGEM EAGLAVEGGS SEMKMIVSYV DSLPTGGEEG SYYALDLGGT NFRVLRVRLA
GGGVAERVAR EVPIPPGLMS GGGATSECLF GFIASALAEF VGEEEEEGGL DGGERELGFT
FSFPVHQTSI ASGTLIRWTK AFAVDDAIGE DVVAALQAAM SERGLDMRVS ALINDTVGTL
AAGSYYDEDV VAAVILGTGT NAAYVEDATA IAKLHPSQLP ASNTMVINTE WGSFASPCLP
LTEFDEALDQ ESLNPGEQTY EKLISGMYLG EIVRRVLLKI SSRCPSLLGG AGELATPFVL
RTPDVSAMHH DETPDLSIVG EKLERTLGIR GTSPEARRMV VEVCDIVATR AARLAAAGIV
GILKKIGRVD GGEGRRRRSV VAVDGGLFEH YGKFRRCMES AVRELLGEAA AERVVVKLAS
DGSGLGAALV AAAHSQRA
