HXK2_SOLTU
ID HXK2_SOLTU Reviewed; 496 AA.
AC Q9SQ76;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Hexokinase-2;
DE EC=2.7.1.1 {ECO:0000305|PubMed:12090625};
DE AltName: Full=StHK2 {ECO:0000303|PubMed:12090625};
GN Name=HXK2;
OS Solanum tuberosum (Potato).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=4113;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=12090625; DOI=10.1023/a:1015528014562;
RA Veramendi J., Fernie A.R., Leisse A., Willmitzer L., Trethewey R.N.;
RT "Potato hexokinase 2 complements transgenic Arabidopsis plants deficient in
RT hexokinase 1 but does not play a key role in tuber carbohydrate
RT metabolism.";
RL Plant Mol. Biol. 49:491-501(2002).
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme. May be involved
CC in the phosphorylation of glucose during the export from plastids to
CC cytosol. Seems neither to be involved in cell sugar sensing nor in
CC carbohydrate metabolism in tuber. {ECO:0000269|PubMed:12090625}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:12090625};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000305|PubMed:12090625};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:12090625};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000305|PubMed:12090625};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:12090625};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000305|PubMed:12090625};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000305|PubMed:12090625}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000305|PubMed:12090625}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
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DR EMBL; AF106068; AAF14186.1; -; mRNA.
DR RefSeq; NP_001274841.1; NM_001287912.1.
DR AlphaFoldDB; Q9SQ76; -.
DR SMR; Q9SQ76; -.
DR STRING; 4113.PGSC0003DMT400042598; -.
DR PRIDE; Q9SQ76; -.
DR EnsemblPlants; RHC06H1G2227.2.1; RHC06H1G2227.2.1; RHC06H1G2227.2.
DR GeneID; 102577690; -.
DR Gramene; RHC06H1G2227.2.1; RHC06H1G2227.2.1; RHC06H1G2227.2.
DR KEGG; sot:102577690; -.
DR eggNOG; KOG1369; Eukaryota.
DR InParanoid; Q9SQ76; -.
DR OrthoDB; 1153545at2759; -.
DR BRENDA; 2.7.1.1; 5757.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000011115; Unassembled WGS sequence.
DR ExpressionAtlas; Q9SQ76; baseline.
DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR GO; GO:0004340; F:glucokinase activity; IBA:GO_Central.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR GO; GO:0001678; P:cellular glucose homeostasis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00378; HEXOKINASE_1; 1.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Glycolysis; Kinase; Membrane; Nucleotide-binding;
KW Plastid; Plastid outer membrane; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..496
FT /note="Hexokinase-2"
FT /id="PRO_0000197616"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 35..487
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 90..228
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 171..197
FT /note="Glucose-binding"
FT /evidence="ECO:0000255"
FT REGION 229..476
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT BINDING 101..106
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 496 AA; 53722 MW; 786F08093EEE5B8C CRC64;
MKKATVGAVV VGTAAAVAVA ALIMRHRMGK SSKWARARAI LKEFEEKCAT PDGKLKQVAD
AMTVEMHAGL ASEGGSKLKM LISYVDNLPT GDEGGVFYAL DLGGTNFRVL RVQLGGKDGG
IIHQEFAEAS IPPNLMVGTS EALFDYIAAE LAKFVAEEGE EFHPPPGRQR ELGFTFSFPI
MQTSINSGTL IRWTKGFSID DTVGKDVVAE LTKAMQKREI DMRVSALVND TVGTLAGGRF
TNKDVSIAVI LGTGTNAAYV ERAQAIPKWH GPLPKSGEMV INMEWGNFRS SHLPLTEYDH
AMDTNSLNPG EQIFEKICSG MYLGEILRRV LLRMAEEAGI FGEEVPPKLK NSFILRTPEM
SAMHHDTSSD LRVVGDKLKD ILEISNSSLK TRRLVVELCN IVATRGARLA AAGILGIIKK
MGKDTPRESG PEKIVVAMDG GLYEHYTEYS KCLENTLVEL LGKEMATSIV FKHANDGSGI
GAALLAASNS VYVEDK
