HXK2_TOBAC
ID HXK2_TOBAC Reviewed; 499 AA.
AC Q6Q8A5;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Hexokinase-2, chloroplastic;
DE EC=2.7.1.1 {ECO:0000305|PubMed:15670855};
DE AltName: Full=NtHxK2;
DE Flags: Precursor;
GN Name=HXK2;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=15670855; DOI=10.1016/j.febslet.2004.12.071;
RA Giese J.-O., Herbers K., Hoffmann M., Kloesgen R.B., Sonnewald U.;
RT "Isolation and functional characterization of a novel plastidic hexokinase
RT from Nicotiana tabacum.";
RL FEBS Lett. 579:827-831(2005).
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme.
CC {ECO:0000269|PubMed:15670855}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:15670855};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000305|PubMed:15670855};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:15670855};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000305|PubMed:15670855};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:15670855};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000305|PubMed:15670855};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000305|PubMed:15670855}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000305|PubMed:15670855}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000305|PubMed:15670855}.
CC -!- TISSUE SPECIFICITY: Expressed in vascular starch sheath, xylem
CC parenchyma, guard cells and root tips. {ECO:0000269|PubMed:15670855}.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
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DR EMBL; AY553215; AAS60193.1; -; mRNA.
DR RefSeq; NP_001312431.1; NM_001325502.1.
DR AlphaFoldDB; Q6Q8A5; -.
DR SMR; Q6Q8A5; -.
DR STRING; 4097.Q6Q8A5; -.
DR PRIDE; Q6Q8A5; -.
DR GeneID; 107790581; -.
DR KEGG; nta:107790581; -.
DR OMA; VCSIDLH; -.
DR BioCyc; MetaCyc:MON-12872; -.
DR BRENDA; 2.7.1.1; 3645.
DR SABIO-RK; Q6Q8A5; -.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR GO; GO:0004340; F:glucokinase activity; IBA:GO_Central.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR GO; GO:0001678; P:cellular glucose homeostasis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Glycolysis; Kinase; Nucleotide-binding; Plastid;
KW Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..499
FT /note="Hexokinase-2, chloroplastic"
FT /id="PRO_0000247574"
FT DOMAIN 39..490
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 94..232
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 233..479
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
SQ SEQUENCE 499 AA; 53997 MW; FE40C67B74EA4812 CRC64;
MSVTVSSPAG RSFHISRSPY KKISKPRVII AAVRSGVSLA VAPILTKLQK DCATPLPVLR
HVADAMAVDM RAGLAVDGGS DLKMILSYID TLPTGNEKGL FYALDLGGTN FRVLRVQLGG
KEERVIATEF EQVSIPQELM FATSEELFDF IASELGKFSQ SEGGKFEMQQ GRTREIGFTF
SFPVKQTSVK SGILIKWTKG FAVSGTAGKD VVACLNEAME RQGLGMQVSA LVNDTVATLA
GARYWDNDVM VAVILGTGTN ACYVERVDAI PKLPQRMSNS PETIVNTEWG AFSNGLPLTE
FDREMDAESI NPGEQIFEKT ISGMYLGEIV RRVLVKMAKV GGLFGGGYVP EKLVTPFVLR
TPDICAMQQD TSRDLEAVES VLYDIAGVKS DLSARKTVVD ICDTIANRGG RLAGAGIVGI
LQKMEEDSKG VIFGKRTVVA MDGGLYEHYP QYREYLQEAV TELLGSEISK NVVIEHSKDG
SGIGAALLAA ANSKYEHDY
