HXK3_ORYSJ
ID HXK3_ORYSJ Reviewed; 500 AA.
AC Q2KNB4; Q5JLP7;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Hexokinase-3;
DE EC=2.7.1.1 {ECO:0000305|PubMed:16552590};
DE AltName: Full=Hexokinase-8;
GN Name=HXK3; Synonyms=HXK8; OrderedLocusNames=Os01g0940100, LOC_Os01g71320;
GN ORFNames=B1150F11.26-1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, INDUCTION, AND NOMENCLATURE.
RC STRAIN=cv. Jinmi;
RX PubMed=16552590; DOI=10.1007/s00425-006-0251-y;
RA Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H.,
RA Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S.;
RT "Structure, expression, and functional analysis of the hexokinase gene
RT family in rice (Oryza sativa L.).";
RL Planta 224:598-611(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Zhonghua 15; TISSUE=Flower;
RA Wang Y.D., Cheng W., Wang X.S., Zhou X.J.;
RT "The hexokinase gene family in rice.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme.
CC {ECO:0000269|PubMed:16552590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000305|PubMed:16552590}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000305|PubMed:16552590}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, flowers, immature seeds
CC and seed coat. {ECO:0000269|PubMed:16552590}.
CC -!- DEVELOPMENTAL STAGE: Expressed during flower development until 8 days
CC after flowering. {ECO:0000269|PubMed:16552590}.
CC -!- INDUCTION: Not induced by glucose or fructose treatment in leaves.
CC {ECO:0000269|PubMed:16552590}.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD87613.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ116385; AAZ93620.1; -; mRNA.
DR EMBL; AY884171; AAX68424.1; -; mRNA.
DR EMBL; AP003412; BAD87613.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015621344.1; XM_015765858.1.
DR AlphaFoldDB; Q2KNB4; -.
DR SMR; Q2KNB4; -.
DR STRING; 4530.OS01T0940100-01; -.
DR PaxDb; Q2KNB4; -.
DR PRIDE; Q2KNB4; -.
DR GeneID; 4326516; -.
DR KEGG; osa:4326516; -.
DR eggNOG; KOG1369; Eukaryota.
DR InParanoid; Q2KNB4; -.
DR OrthoDB; 1153545at2759; -.
DR BRENDA; 2.7.1.1; 4460.
DR PlantReactome; R-OSA-1119570; Cytosolic glycolysis.
DR PlantReactome; R-OSA-1119595; Mannose degradation.
DR PlantReactome; R-OSA-1119601; Trehalose degradation II.
DR PlantReactome; R-OSA-1119628; GDP-mannose metabolism.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q2KNB4; OS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IEA:RHEA.
DR GO; GO:0004340; F:glucokinase activity; IEA:RHEA.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0001678; P:cellular glucose homeostasis; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00378; HEXOKINASE_1; 1.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Glycolysis; Kinase; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Nucleotide-binding; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..500
FT /note="Hexokinase-3"
FT /id="PRO_0000247566"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 35..487
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 90..222
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 165..191
FT /note="Glucose-binding"
FT /evidence="ECO:0000255"
FT REGION 223..476
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT BINDING 101..106
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 500 AA; 53794 MW; 0179231A6AC47A2C CRC64;
MGRVGLGVAV GCAAVTCAIA AALVARRASA RARWRRAVAL LREFEEGCAT PPARLRQVVD
AMVVEMHAGL ASDGGSKLKM LLTFVDALPS GSEEGVYYSI DLGGTNFRVL RVQVGAGSVI
VNQKVEQQPI PEELTKGTTE GLFNFVALAL KNFLEGEDDQ DGKMALGFTF SFPVRQISVS
SGSLIRWTKG FSIRDTVGRD VAQCLNEALA NCGLNVRVTA LVNDTVGTLA LGHYYDEDTV
AAVIIGSGTN ACYIERTDAI IKCQGLLTNS GGMVVNMEWG NFWSSHLPRT PYDILLDDET
HNRNDQGFEK MISGMYLGEI ARLVFHRMAQ ESDVFGDAAD SLSNPFILST PFLAAIREDD
SPDLSEVRRI LREHLKIPDA PLKTRRLVVK VCDIVTRRAA RLAAAGIVGI LKKLGRDGSG
AASSGRGRGQ PRRTVVAIEG GLYQGYPVFR EYLDEALVEI LGEEVARNVT LRVTEDGSGV
GAALLAAVHS SNRQQQGGPI
