HXK6_ORYSJ
ID HXK6_ORYSJ Reviewed; 506 AA.
AC Q8LQ68; Q0JJF0;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Hexokinase-6;
DE EC=2.7.1.1 {ECO:0000305|PubMed:16552590};
DE AltName: Full=Hexokinase-2;
GN Name=HXK6; Synonyms=HXK2; OrderedLocusNames=Os01g0742500, LOC_Os01g53930;
GN ORFNames=P0439E07.19;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, INDUCTION, AND NOMENCLATURE.
RC STRAIN=cv. Jinmi;
RX PubMed=16552590; DOI=10.1007/s00425-006-0251-y;
RA Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H.,
RA Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S.;
RT "Structure, expression, and functional analysis of the hexokinase gene
RT family in rice (Oryza sativa L.).";
RL Planta 224:598-611(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Zhonghua 15; TISSUE=Flower;
RA Wang Y.D., Cheng W., Wang X.S., Zhou X.J.;
RT "The hexokinase gene family in rice.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme.
CC {ECO:0000269|PubMed:16552590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000305|PubMed:16552590};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000305|PubMed:16552590};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000305|PubMed:16552590}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000305|PubMed:16552590}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, flowers, immature seeds
CC and endosperm. {ECO:0000269|PubMed:16552590}.
CC -!- DEVELOPMENTAL STAGE: Expressed during flower development until 15 days
CC after flowering. {ECO:0000269|PubMed:16552590}.
CC -!- INDUCTION: By glucose or fructose treatment in leaves.
CC {ECO:0000269|PubMed:16552590}.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
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DR EMBL; DQ116388; AAZ93623.1; -; mRNA.
DR EMBL; AY884165; AAX68418.1; -; mRNA.
DR EMBL; AP003768; BAB91930.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06128.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS74288.1; -; Genomic_DNA.
DR EMBL; AK065656; BAG89607.1; -; mRNA.
DR RefSeq; XP_015618116.1; XM_015762630.1.
DR PDB; 5ZQT; X-ray; 2.84 A; A/B/C=45-506.
DR PDB; 6JJ4; X-ray; 2.60 A; A=40-506.
DR PDB; 6JJ7; X-ray; 2.90 A; A/C/E=39-503.
DR PDB; 6JJ8; X-ray; 2.80 A; A/B/C=39-503.
DR PDB; 6JJ9; X-ray; 3.00 A; A/C/E=39-503.
DR PDBsum; 5ZQT; -.
DR PDBsum; 6JJ4; -.
DR PDBsum; 6JJ7; -.
DR PDBsum; 6JJ8; -.
DR PDBsum; 6JJ9; -.
DR AlphaFoldDB; Q8LQ68; -.
DR SMR; Q8LQ68; -.
DR STRING; 4530.OS01T0742500-01; -.
DR PaxDb; Q8LQ68; -.
DR PRIDE; Q8LQ68; -.
DR EnsemblPlants; Os01t0742500-01; Os01t0742500-01; Os01g0742500.
DR GeneID; 4326547; -.
DR Gramene; Os01t0742500-01; Os01t0742500-01; Os01g0742500.
DR KEGG; osa:4326547; -.
DR eggNOG; KOG1369; Eukaryota.
DR HOGENOM; CLU_014393_5_1_1; -.
DR InParanoid; Q8LQ68; -.
DR OMA; FCNETAK; -.
DR OrthoDB; 1153545at2759; -.
DR BRENDA; 2.7.1.1; 4460.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q8LQ68; OS.
DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:CACAO.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR GO; GO:0004340; F:glucokinase activity; IMP:CACAO.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR GO; GO:0001678; P:cellular glucose homeostasis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR GO; GO:0009749; P:response to glucose; IMP:CACAO.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00378; HEXOKINASE_1; 1.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Chloroplast; Glycolysis; Kinase; Membrane;
KW Nucleotide-binding; Plastid; Plastid outer membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..506
FT /note="Hexokinase-6"
FT /id="PRO_0000247569"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 43..497
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 98..236
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 179..205
FT /note="Glucose-binding"
FT /evidence="ECO:0000255"
FT REGION 237..486
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT BINDING 109..114
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 49..55
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 60..77
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 102..110
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 112..123
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 124..127
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 129..138
FT /evidence="ECO:0007829|PDB:6JJ4"
FT TURN 141..144
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 145..147
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 148..163
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 172..175
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 178..184
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 188..192
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 195..198
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 208..210
FT /evidence="ECO:0007829|PDB:5ZQT"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 215..224
FT /evidence="ECO:0007829|PDB:6JJ4"
FT TURN 225..227
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 230..236
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 238..249
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 253..271
FT /evidence="ECO:0007829|PDB:6JJ4"
FT TURN 275..277
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 279..281
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 285..290
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 293..295
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 305..312
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 314..320
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 323..326
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 328..344
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 350..352
FT /evidence="ECO:0007829|PDB:5ZQT"
FT HELIX 356..358
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 365..372
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 376..378
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 380..389
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 397..428
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 436..439
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 444..450
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 451..455
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 457..470
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 473..476
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 479..483
FT /evidence="ECO:0007829|PDB:6JJ4"
FT STRAND 487..489
FT /evidence="ECO:0007829|PDB:6JJ4"
FT HELIX 493..496
FT /evidence="ECO:0007829|PDB:6JJ4"
SQ SEQUENCE 506 AA; 55121 MW; 242F8DCCB5FF8947 CRC64;
MGKGTVVGTA VVVCAAAAAA VGVAVVVSRR RRSKREAEEE RRRRAAAVIE EVEQRFSTPT
ALLRGIADAM VEEMERGLRA DPHAPLKMLI SYVDNLPTGD EHGLFYALDL GGTNFRVIRV
QLGGREKRVV SQQYEEVAIP PHLMVGTSME LFDFIAAELE SFVKTEGEDF HLPEGRQREL
GFTFSFPVHQ TSISSGTLIK WTKGFSINGT VGEDVVAELS RAMERQGLDM KVTALVNDTV
GTLAGGRYVD NDVAAAVILG TGTNAAYVEH ANAIPKWTGL LPRSGNMVIN MEWGNFKSER
LPRSDYDNAL DFESLNPGEQ IYEKMISGMY LGEIVRRILL KLAHDASLFG DVVPTKLEQR
FILRTPDMSA MHHDTSHDLK HLGAKLKDIL GVADTSLEAR YITLHVCDLV AERGARLAAA
GIYGILKKLG RDRVPSDGSQ KQRTVIALDG GLYEHYKKFR TCLEATLADL LGEEAASSVV
VKLANDGSGI GAALLAASHS QYASVE
