HXKL1_ARATH
ID HXKL1_ARATH Reviewed; 493 AA.
AC Q9FZG4; A4VCN1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Hexokinase-like 1 protein;
DE EC=2.7.1.1 {ECO:0000250|UniProtKB:P93834};
GN OrderedLocusNames=At1g47840; ORFNames=T2E6.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista-Mercan V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C.,
RA Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Fructose and glucose phosphorylating enzyme.
CC {ECO:0000250|UniProtKB:P93834}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000250|UniProtKB:P93834};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000250|UniProtKB:P93834};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000250|UniProtKB:P93834};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000250|UniProtKB:P93834}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000250|UniProtKB:P93834}.
CC -!- SIMILARITY: Belongs to the hexokinase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01084, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF99786.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012463; AAF99786.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32221.1; -; Genomic_DNA.
DR EMBL; BT030472; ABP88126.1; -; mRNA.
DR RefSeq; NP_175220.2; NM_103682.4.
DR AlphaFoldDB; Q9FZG4; -.
DR SMR; Q9FZG4; -.
DR STRING; 3702.AT1G47840.1; -.
DR PaxDb; Q9FZG4; -.
DR PRIDE; Q9FZG4; -.
DR ProteomicsDB; 232195; -.
DR EnsemblPlants; AT1G47840.1; AT1G47840.1; AT1G47840.
DR GeneID; 841200; -.
DR Gramene; AT1G47840.1; AT1G47840.1; AT1G47840.
DR KEGG; ath:AT1G47840; -.
DR Araport; AT1G47840; -.
DR TAIR; locus:2202410; AT1G47840.
DR eggNOG; KOG1369; Eukaryota.
DR HOGENOM; CLU_014393_5_1_1; -.
DR InParanoid; Q9FZG4; -.
DR OMA; VCSIDLH; -.
DR OrthoDB; 1153545at2759; -.
DR PhylomeDB; Q9FZG4; -.
DR BioCyc; ARA:AT1G47840-MON; -.
DR BRENDA; 2.7.1.1; 399.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR PRO; PR:Q9FZG4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FZG4; baseline and differential.
DR Genevisible; Q9FZG4; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IDA:TAIR.
DR GO; GO:0004340; F:glucokinase activity; IDA:TAIR.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR GO; GO:0001678; P:cellular glucose homeostasis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; PTHR19443; 1.
DR Pfam; PF00349; Hexokinase_1; 1.
DR Pfam; PF03727; Hexokinase_2; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00378; HEXOKINASE_1; 1.
DR PROSITE; PS51748; HEXOKINASE_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Glycolysis; Kinase; Nucleotide-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..493
FT /note="Hexokinase-like 1 protein"
FT /id="PRO_0000259633"
FT DOMAIN 38..488
FT /note="Hexokinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 93..232
FT /note="Hexokinase small subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT REGION 175..201
FT /note="Glucose-binding"
FT /evidence="ECO:0000255"
FT REGION 233..477
FT /note="Hexokinase large subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01084"
FT BINDING 104..109
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 493 AA; 53880 MW; 485C0BA6FBCDECF9 CRC64;
MSLMFSSPVV TPALGSFTFS SRPRSNYIVM SAVRSNSAST CPILTKFQKD CATPTPYLRN
VANAIADDMR DGLAVEGGGD LEMILTFVDA LPSGNEEGLF YALDLGGTNF RVRSVQLGGK
KERVLATESE QISISQKLMI GTSEELFGFI ASKLANFVAK EKPGRFLLEE GRKRELGFTF
SFPVKQTSID SGTLSKWTKG FKVSGMEGKN VVACLNEAME AHGLDMRVSA LVNDGVGTLA
GARYWDEDVM VGVILGTGTN ACYVEQKHAI PKLRSKSSSG TTIINTEWGG FSKILPQTIF
DLEMDETSLN PGEHLYEKMI SGMYLGEIVR RVLLHMCETS DLFGHFAPAK LSTPLALRTE
HLCKMQEDNT DDLRDVGSIL YDFLDVEANM NARRRVVEVC DTVVKRGGRL AGAGIVAILE
KIEKDTKRMG SGKRTVVAMD GALYEKYPQY RQYMQDALVE LLGHKLASHV AIKHTKDVSG
LGAALLAATN SIY
