HXNR_EMENI
ID HXNR_EMENI Reviewed; 865 AA.
AC C8VJW0;
DT 28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=Nicotinate catabolism cluster-specific transcription factor {ECO:0000303|PubMed:29212709};
GN Name=hxnR {ECO:0000303|PubMed:29212709};
GN Synonyms=aplA {ECO:0000303|PubMed:4581274}; ORFNames=ANIA_11197;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP FUNCTION.
RX PubMed=4581274; DOI=10.1111/j.1432-1033.1973.tb02928.x;
RA Scazzocchio C., Holl F.B., Foguelman A.I.;
RT "The genetic control of molybdoflavoproteins in Aspergillus nidulans.
RT Allopurinol-resistant mutants constitutive for xanthine-dehydrogenase.";
RL Eur. J. Biochem. 36:428-445(1973).
RN [4]
RP IDENTIFICATION, INDUCTION, FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS
RP OF PRO-219; TYR-226; ASN-227; TRP-228; PHE-230; ASP-237; ALA-238; PHE-239;
RP PHE-565; LYS-603; THR-607 AND ARG-639.
RX PubMed=29212709; DOI=10.1098/rsob.170199;
RA Amon J., Fernandez-Martin R., Bokor E., Cultrone A., Kelly J.M.,
RA Flipphi M., Scazzocchio C., Hamari Z.;
RT "A eukaryotic nicotinate-inducible gene cluster: convergent evolution in
RT fungi and bacteria.";
RL Open Biol. 7:0-0(2017).
CC -!- FUNCTION: Transcription factor that specifically regulates the
CC expression of the hxn gene cluster that mediates the degradation of
CC nicotinate and related metabolites (PubMed:4581274, PubMed:29212709).
CC {ECO:0000269|PubMed:29212709, ECO:0000269|PubMed:4581274}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:29212709}.
CC -!- INDUCTION: Expression is induced by nicotinate and 6-OH nicotinate and
CC is subject to nitrogen metabolite repression mediated by the GATA
CC factor areA (PubMed:29212709). {ECO:0000269|PubMed:29212709}.
CC -!- DISRUPTION PHENOTYPE: Leads to the inability to use nicotinate, 6-OH
CC nicotinic acid, and 2,5-dihydroxypyridine as sole nitrogen sources
CC (PubMed:29212709). {ECO:0000269|PubMed:29212709}.
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DR EMBL; BN001306; CBF82382.1; -; Genomic_DNA.
DR AlphaFoldDB; C8VJW0; -.
DR SMR; C8VJW0; -.
DR EnsemblFungi; CBF82382; CBF82382; ANIA_11197.
DR VEuPathDB; FungiDB:AN11197; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_005733_1_1_1; -.
DR InParanoid; C8VJW0; -.
DR OMA; IRRSNCC; -.
DR OrthoDB; 319562at2759; -.
DR Proteomes; UP000000560; Chromosome VI.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF04082; Fungal_trans; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..865
FT /note="Nicotinate catabolism cluster-specific transcription
FT factor"
FT /id="PRO_0000443341"
FT ZN_FING 8..32
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 41..63
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 74..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 77..87
FT /note="Nuclear localization signal(NLS)"
FT /evidence="ECO:0000303|PubMed:29212709"
FT MOTIF 285..289
FT /note="Nuclear export signal (NES)"
FT /evidence="ECO:0000303|PubMed:29212709"
FT COMPBIAS 74..96
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..135
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 219
FT /note="P->A,L: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 226
FT /note="Y->D,L,S: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 227
FT /note="N->D: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 228
FT /note="W->R,S: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 230
FT /note="F->I,S: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 237
FT /note="D->Y: Leads to constitutive activator activity; when
FT associated with Pro-238."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 238
FT /note="A->P: Leads to constitutive activator activity; when
FT associated with Tyr-237."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 239
FT /note="F->S: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 565
FT /note="F->S: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 603
FT /note="K->N,E,T: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 607
FT /note="T->P,S: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
FT MUTAGEN 639
FT /note="R->C: Leads to constitutive activator activity."
FT /evidence="ECO:0000269|PubMed:29212709"
SQ SEQUENCE 865 AA; 94673 MW; A5E16296A17BCEBE CRC64;
MKAKMKKHAC TYPGCSKAFT RAEHLRRHSL NHETISNSQG YTCQRCMTHF SRADLLSRHL
DRHAKKDAEA GGFGKGVLET RKRMRRAEDG SIVLRPPKRP SRHQQKTGPP VGAPLSSSGS
VSAGSGRSSR SPDVSLHAAQ APVSPPRSAS DPVSVSGVSI DDDGTDPDPM LAPMMPGGPF
EPYVEPIPGQ FDAADGSWGG FDALGDGMML DTATDFNLPF AATGNYNWLF DVSSLDDAFH
HLELPLGPDL VPFANSHGNY ASVNTMELSG AGAENVQDSM LNLDLDIDLN GLPAGFVHDQ
GPDGSSASVL LQAASFVERG NINGSDPKRD FPDLDWMAGA PPIESTVPLR PQLSEDARRG
ILTLIAQSPP VDIHGQPLNL DSPLLSLSAL QSYSDLFFSR FNTTYPLIHS ATFDPNKTEP
VFLASILSMG ATYSSREAHQ LAVGIHDGLR NQLFCHGAFS PQPDELWVLQ AMLLIDCFGK
MRAGPKQRER AQLFHCVLIK LIRRSTCCSI RADTHSDPGL GGLELEDAWK RAMDAEQRKR
LAFQCFMWDT EHSVLFSQSL CMSAFEIRSS LPCSPAAWEA HTAEEWSRHA SRDTEHAFLP
VLKGYITPGS VSRPRDLNRF SRMVVLHGLM SISADLKRRD QTTLRAETPE RVGAWTPRMG
RAYDLWKADF DADCLNMKLG PVQVSADETR RFTSLKAAAM ALYRAASLAL HVEVLDLQIA
AGASHILGRV VKQHDRERSR VMLSRWLSGP SPAATTASRH AAALLQDAVL SLHDWDQTDA
FHFPWCLYLA TLTVWAFHAR EGCVPKPTDL SSLIVAMTTS NAADLEGLAG QYDTRPLIRA
MAQQLATVRW AVVHDAMKVL LNLGV
