HXT8_YEAST
ID HXT8_YEAST Reviewed; 569 AA.
AC P40886; D6VVY1;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Hexose transporter HXT8;
GN Name=HXT8; OrderedLocusNames=YJL214W; ORFNames=HRA569, J0232;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7725802; DOI=10.1002/yea.320101216;
RA Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.;
RT "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere
RT of yeast chromosome X.";
RL Yeast 10:1657-1662(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Probable glucose transporter.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; Z34098; CAA83997.1; -; Genomic_DNA.
DR EMBL; Z49489; CAA89511.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08597.1; -; Genomic_DNA.
DR PIR; S50771; S50771.
DR RefSeq; NP_012321.1; NM_001181647.1.
DR AlphaFoldDB; P40886; -.
DR SMR; P40886; -.
DR BioGRID; 33545; 61.
DR DIP; DIP-8121N; -.
DR IntAct; P40886; 1.
DR MINT; P40886; -.
DR STRING; 4932.YJL214W; -.
DR PaxDb; P40886; -.
DR EnsemblFungi; YJL214W_mRNA; YJL214W; YJL214W.
DR GeneID; 853216; -.
DR KEGG; sce:YJL214W; -.
DR SGD; S000003750; HXT8.
DR VEuPathDB; FungiDB:YJL214W; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176280; -.
DR HOGENOM; CLU_001265_30_1_1; -.
DR InParanoid; P40886; -.
DR OMA; MIFITCC; -.
DR BioCyc; YEAST:G3O-31641-MON; -.
DR PRO; PR:P40886; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P40886; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IBA:GO_Central.
DR GO; GO:0005353; F:fructose transmembrane transporter activity; IMP:SGD.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IMP:SGD.
DR GO; GO:0015578; F:mannose transmembrane transporter activity; IMP:SGD.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0008645; P:hexose transmembrane transport; IMP:SGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Repeat; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..569
FT /note="Hexose transporter HXT8"
FT /id="PRO_0000050398"
FT TOPO_DOM 1..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..118
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..238
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..359
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 360..365
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..383
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 412..433
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..471
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 493
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..569
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 429
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 569 AA; 63493 MW; 391C2DE8E9DB0FF2 CRC64;
MTDRKTNLPE EPIFEEAEDD GCPSIENSSH LSVPTVEENK DFSEYNGEEA EEVVVPEKPA
SAYATVSIMC LCMAFGGFMS GWDTGTISGF VNQTDFLRRF GNYSHSKNTY YLSNVRTGLI
VSIFNVGSAI GCLFLSKLGD IYGRCMGLII VIVVYMVGIV IQIASIDKWY QYFIGRIIAG
IGAGSISVLA PMLISETAPK HIRGTLLACW QLMVTFAIFL GYCTNYGTKT YSNSVQWRVP
LGLCFAWAII MIGGMTFVPE SPRFLVQVGK IEQAKASFAK SNKLSVDDPA VVAEIDLLVA
GVEAEEAMGT ASWKELFSRK TKVFQRLTMT VMINSLQQLT GDNYFFYYGT TIFKSVGMND
SFETSIVLGI VNFASCFFSL YSVDKLGRRR CLLLGAATMT ACMVIYASVG VTRLYPNGKS
EPSSKGAGNC TIVFTCFYIF CFSCTWGPVC YVIISETFPL RVRSKCMSVA TAANLLWGFL
IGFFTPFITS AINFYYGYVF MGCLAFSYFY VFFFVPETKG LTLEEVDEMW MDGVLPWKSE
SWVPASRRDG DYDNEKLQHD EKPFYKRMF
