HYAD_ECOLI
ID HYAD_ECOLI Reviewed; 195 AA.
AC P19930;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Hydrogenase 1 maturation protease;
DE EC=3.4.23.-;
GN Name=hyaD; OrderedLocusNames=b0975, JW0957;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2180913; DOI=10.1128/jb.172.4.1969-1977.1990;
RA Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S.,
RA Przybyla A.E.;
RT "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1
RT operon containing six open reading frames.";
RL J. Bacteriol. 172:1969-1977(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Protease involved in the C-terminal processing of HyaB, the
CC large subunit of hydrogenase 1. {ECO:0000305}.
CC -!- INTERACTION:
CC P19930; P0ACD8: hyaB; NbExp=3; IntAct=EBI-552940, EBI-851493;
CC P19930; P00956: ileS; NbExp=3; IntAct=EBI-552940, EBI-552928;
CC -!- SIMILARITY: Belongs to the peptidase A31 family. {ECO:0000305}.
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DR EMBL; M34825; AAA24000.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74060.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35740.1; -; Genomic_DNA.
DR PIR; JV0075; QQECHD.
DR RefSeq; NP_415494.1; NC_000913.3.
DR RefSeq; WP_000003671.1; NZ_STEB01000006.1.
DR AlphaFoldDB; P19930; -.
DR SMR; P19930; -.
DR BioGRID; 4260045; 21.
DR BioGRID; 849949; 13.
DR DIP; DIP-9960N; -.
DR IntAct; P19930; 22.
DR STRING; 511145.b0975; -.
DR MEROPS; A31.002; -.
DR jPOST; P19930; -.
DR PaxDb; P19930; -.
DR PRIDE; P19930; -.
DR EnsemblBacteria; AAC74060; AAC74060; b0975.
DR EnsemblBacteria; BAA35740; BAA35740; BAA35740.
DR GeneID; 66670749; -.
DR GeneID; 945575; -.
DR KEGG; ecj:JW0957; -.
DR KEGG; eco:b0975; -.
DR PATRIC; fig|1411691.4.peg.1299; -.
DR EchoBASE; EB0466; -.
DR eggNOG; COG0680; Bacteria.
DR HOGENOM; CLU_099037_0_1_6; -.
DR InParanoid; P19930; -.
DR OMA; MGAKKMS; -.
DR PhylomeDB; P19930; -.
DR BioCyc; EcoCyc:EG10471-MON; -.
DR PRO; PR:P19930; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0004175; F:endopeptidase activity; IBA:GO_Central.
DR GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0036211; P:protein modification process; IBA:GO_Central.
DR GO; GO:0016485; P:protein processing; IMP:EcoCyc.
DR Gene3D; 3.40.50.1450; -; 1.
DR InterPro; IPR004419; Pept_A31_hyd_express.
DR InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR InterPro; IPR000671; Peptidase_A31.
DR PANTHER; PTHR30302; PTHR30302; 1.
DR Pfam; PF01750; HycI; 1.
DR PRINTS; PR00446; HYDRGNUPTAKE.
DR SUPFAM; SSF53163; SSF53163; 1.
DR TIGRFAMs; TIGR00140; hupD; 1.
DR TIGRFAMs; TIGR00072; hydrog_prot; 1.
PE 1: Evidence at protein level;
KW Aspartyl protease; Hydrolase; Metal-binding; Nickel; Protease;
KW Reference proteome.
FT CHAIN 1..195
FT /note="Hydrogenase 1 maturation protease"
FT /id="PRO_0000201942"
FT BINDING 19
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 65
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 96
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
SQ SEQUENCE 195 AA; 21546 MW; 119B3BAB53A9186F CRC64;
MSEQRVVVMG LGNLLWADEG FGVRVAERLY AHYHWPEYVE IVDGGTQGLN LLGYVESASH
LLILDAIDYG LEPGTLRTYA GERIPAYLSA KKMSLHQNSF SEVLALADIR GHLPAHIALV
GLQPAMLDDY GGSLSELARE QLPAAEQAAL AQLAAWGIVP QPANESRCLN YDCLSMENYE
GVRLRQYRMT QEEQG
