ID   HYAF_ECOLI              Reviewed;         285 AA.
AC   P19932;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Hydrogenase-1 operon protein HyaF;
GN   Name=hyaF; OrderedLocusNames=b0977, JW0959;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2180913; DOI=10.1128/jb.172.4.1969-1977.1990;
RA   Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S.,
RA   Przybyla A.E.;
RT   "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1
RT   operon containing six open reading frames.";
RL   J. Bacteriol. 172:1969-1977(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Not known. Could enhance the incorporation of nickel to the
CC       hydrogenase.
CC   -!- SIMILARITY: Belongs to the HupH/HyaF family. {ECO:0000305}.
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DR   EMBL; M34825; AAA24002.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74062.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35742.1; -; Genomic_DNA.
DR   PIR; JV0077; QQECHF.
DR   RefSeq; NP_415496.1; NC_000913.3.
DR   RefSeq; WP_000004899.1; NZ_STEB01000006.1.
DR   AlphaFoldDB; P19932; -.
DR   SMR; P19932; -.
DR   BioGRID; 4260047; 21.
DR   BioGRID; 849946; 2.
DR   IntAct; P19932; 9.
DR   STRING; 511145.b0977; -.
DR   jPOST; P19932; -.
DR   PaxDb; P19932; -.
DR   PRIDE; P19932; -.
DR   EnsemblBacteria; AAC74062; AAC74062; b0977.
DR   EnsemblBacteria; BAA35742; BAA35742; BAA35742.
DR   GeneID; 66670747; -.
DR   GeneID; 945572; -.
DR   KEGG; ecj:JW0959; -.
DR   KEGG; eco:b0977; -.
DR   PATRIC; fig|1411691.4.peg.1297; -.
DR   EchoBASE; EB0468; -.
DR   eggNOG; COG1773; Bacteria.
DR   HOGENOM; CLU_079566_0_0_6; -.
DR   InParanoid; P19932; -.
DR   OMA; NFTLLPM; -.
DR   PhylomeDB; P19932; -.
DR   BioCyc; EcoCyc:EG10473-MON; -.
DR   PRO; PR:P19932; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   Gene3D; 3.30.1370.140; -; 2.
DR   InterPro; IPR038527; HupH_C_sf.
DR   InterPro; IPR006894; HupH_Hydgase_express_prot_C.
DR   Pfam; PF04809; HupH_C; 2.
PE   3: Inferred from homology;
KW   Nickel; Reference proteome.
FT   CHAIN           1..285
FT                   /note="Hydrogenase-1 operon protein HyaF"
FT                   /id="PRO_0000201421"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   285 AA;  31431 MW;  BD94FB06730C204A CRC64;
     MSETFFHLLG PGTQPNDDSF SMNPLPITCQ VNDEPSMAAL EQCAHSPQVI ALLNELQHQL
     SERQPPLGEV LAVDLLNLNA DDRHFINTLL GEGEVSVRIQ QADDSESEIQ EAIFCGLWRV
     RRRRGEKLLE DKLEAGCAPL ALWQAATQNL LPTDSLLPPP IDGLMNGLPL AHELLAHVRN
     PDAQPHSINL TQLPISEADR LFLSRLCGPG NIQIRTIGYG ESYINATGLR HVWHLRCTDT
     LKGPLLESYE ICPIPEVVLA APEDLVDSAQ RLSEVCQWLA EAAPT