HYAL_PHOKE
ID HYAL_PHOKE Reviewed; 32 AA.
AC P86274;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 07-OCT-2020, entry version 20.
DE RecName: Full=Hyaluronidase-Pk1a {ECO:0000250|UniProtKB:Q08169};
DE Short=Hya {ECO:0000303|Ref.1};
DE EC=3.2.1.35;
DE AltName: Full=Hyaluronoglucosaminidase {ECO:0000250|UniProtKB:Q08169};
DE Flags: Fragment;
OS Phoneutria keyserlingi (Brazilian wandering spider) (Ctenus keyserlingii).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX NCBI_TaxID=272754;
RN [1]
RP PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Venom {ECO:0000269|Ref.1};
RA Richardson M., Borges M.H., Souza I.A., Cordeiro M.N.;
RT "Hyaluronidase enzyme in venom of spider P.keyserlinigi (wandering).";
RL Submitted (APR-2009) to UniProtKB.
CC -!- FUNCTION: Hydrolyzes high molecular weight hyaluronic acid to produce
CC small oligosaccharides. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC Evidence={ECO:0000269|Ref.1};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.1}.
CC -!- MISCELLANEOUS: On the 2D-gel the MW of this protein is: 37 kDa.
CC {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family. {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR ArachnoServer; AS001206; Hyaluronidase-Pk1a (fragment).
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; Glycosidase;
KW Hydrolase; Secreted.
FT CHAIN 1..>32
FT /note="Hyaluronidase-Pk1a"
FT /id="PRO_0000376024"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 12..?
FT /evidence="ECO:0000250|UniProtKB:Q08169"
FT NON_TER 32
FT /evidence="ECO:0000303|Ref.1"
SQ SEQUENCE 32 AA; 3879 MW; 6CC71A1D7F06AB76 CRC64;
FEVYWNVPTL QCRAVYKMIF KLNRTYGIQX NA